1.1.1.282: quinate/shikimate dehydrogenase

This is an abbreviated version!
For detailed information about quinate/shikimate dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.282

Reaction

L-quinate
+
NAD(P)+
=
3-dehydroquinate
+
NAD(P)H
+
H+

Synonyms

cgl0424, cgR_0495, dehydroquinate dehydratase-shikimate dehydrogenase, More, Poptr2, Poptr3, Poptr4, QSDH, qsuD, quinate/shikimate 5-dehydrogenase, quinate/shikimate dehydrogenase, rifI, RifI2, SDH, SDH/QDH, sdhL, shikimate/quinate dehydrogenase, YdiB

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.282 quinate/shikimate dehydrogenase

Purification

Purification on EC 1.1.1.282 - quinate/shikimate dehydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
3000fold, two forms of the bifunctional quinate (shikimate) dehydrogenase: P1 and P2
-
by nickel-nitrilotriacetic acid affinity chromatography
recombinant enzyme from Escherichia coli
recombinant His6-tagged isozymes from Escherichia coli strain M15 by nickel affinity chromatography
-
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 gold by nickel affinity chromatography, dialysis, tag cleavage through TEV protease and removal by nickel affinity chromatography, followed by gel filtration