1.1.1.286: isocitrate-homoisocitrate dehydrogenase

This is an abbreviated version!
For detailed information about isocitrate-homoisocitrate dehydrogenase, go to the full flat file.

Reaction

(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate
+
NAD+
=
2-oxoadipate
+
CO2
+
NADH
+
H+

Synonyms

3-carboxy-2-hydroxyadipate dehydrogenase, HICDH, homoisocitrate dehydrogenase, isocitrate-homoisocitrate dehydrogenase, More, Saci_2375, TK0280

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.286 isocitrate-homoisocitrate dehydrogenase

Cloned

Cloned on EC 1.1.1.286 - isocitrate-homoisocitrate dehydrogenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
gene saci_2375, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain BL21(DE3)Codon-Plus-RIL
gene TK0280, phylogenetic analysis and tree, cloning in Escherichia coli strain DH5alpha, recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21-CodonPlus (DE3)-RIL
recombinant expression in Escherichia coli strain Escherichia coli BL21(DE3)CodonPlus-RIL