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1.1.1.40: malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)

This is an abbreviated version!
For detailed information about malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+), go to the full flat file.

Word Map on EC 1.1.1.40

Reaction

(S)-malate
+
NADP+
=
pyruvate
 +
CO2
 +
NADPH
 +
H+

Synonyms

ADP-malic enzyme2, c-NADP-ME, C4 NADP-malic enzyme, C4 photosynthetic NADP-malic enzyme, C4-NADP-malic enzyme, C4-NADP-ME, ChlME1, ChlME2, cNAD-ME, cytoNADPME, cytosolic malic enzyme, cytosolic NADP+-dependent isoform, cytosolic NADP+-dependent malic enzyme, L-malate: NADP oxidoreductase (decarboxylating), L-malate: NADP oxidoreductase [OAA decarboxylating], L-malate: NADP oxidoreductase [oxaloacetate decarboxylating], L-malate:NADP oxidoreductase, L-malate:NADP oxidoreductase (oxaloacetate decarboxylating), m-NAD(P)-ME, m-NADP-ME, MaeB, MaeB1, MalA, malate dehydrogenase (decarboxylating, NADP), malate dehydrogenase (NADP, decarboxylating), malE1, malic enzyme, malic enzyme 1, malic enzyme 2, malic enzyme 3, malic enzyme-NADP, ME, ME-61, ME-70, ME-NADP, ME1, ME2, ME3, mitochondrial malic enzyme, mitochondrial NADP malic enzyme, mNAD-ME, NAD(P)+-malic enzyme, NADP dependent malic enzyme, NADP malic enzyme, NADP(+)-dependent mitochondrial malic enzyme 2, NADP(H)-dependent malic enzyme, NADP+ dependent malic enzyme, NADP+-dependent decarboxylating malate dehydrogenase, NADP+-dependent malic enzyme, NADP+-dependent malic enzyme 3, NADP+-dependent ME, NADP+-ME, NADP-dependent malate dehydrogenase, NADP-dependent malic enzyme, NADP-dependent malic enzyme 1, NADP-dependent ME, NADP-linked decarboxylating malic enzyme, NADP-malate dehydrogenase, NADP-malate enzyme, NADP-malic enzyme, NADP-malic enzyme 1, NADP-malic enzyme 2, NADP-MDH, NADP-ME, NADP-ME1, NADP-ME2, NADP-ME3, NADP-ME4, NADP-specific malate dehydrogenase, NADP-specific malic enzyme, NADP-specific ME, NADPH-dependent malic enzyme, NADPH-dependent malic enzyme 1, NADPH-dependent ME1, nicotinamide adenine dinucleotide phosphate-dependent malic enzyme, nicotinamide adenine dinucleotide phosphate-malic enzyme, nonC4-NADP-ME, pNAD-ME, pyruvic-malic carboxylase, RHA1_RS44255, TME

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.40 malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)

Cloned

Cloned on EC 1.1.1.40 - malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
3'-UTR region, cloning, sequencing, and analysis
co-expression in strain W3110 with 5-aminolevulinate synthase, EC 2.3.1.37, from Rhodobacter sphaeroides, hemA gene, for reconstruction of the 5-aminolevulinic acid biosynthesis, subcloning in strain DH5alpha, increased C4 metabolism via NADP-dependent malic enzyme expression results in increased 5-aminolevulinate production by 5-aminolevulinate synthase in Escherichia coli
-
cytosolic and mitochondrial isozymes, expression in of His-tagged isozymes ME1 and ME2 Escherichia coli Rosetta (DE3)pLysS
-
cytosolic and mitochondrial isozymes, expression of His-tagged ME2 in Escherichia coli Rosetta (DE3)pLysS and of His-tagged ME1 in Escherichia coli BL(DE3)
-
DNA and amino acid sequence determination and analysis
DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes in Escherichia coli strain JM109
-
expressed in Arabidopsis thaliana
-
expressed in Chlorella pyrenoidosa
-
expressed in Escherichia coli BL21 (DE3) cells
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(lambdaDE3) cells
-
expressed in Escherichia coli BL21-CodonPlus (DE3) RIL cells
-
expressed in Escherichia coli Rosetta (DE3) pLysS cells
-
expressed in Escherichia coli Rosetta cells
-
expressed in Escherichia coli Rosetta(DE3) cells
expressed in Mucor circinelloides strain MU290
-
expressed in Rhodotorula glutinis strain GM4
expression in Arabidopsis thaliana under control of the 35S promoter
expression in Escherichia coli
-
expression in Escherichia coli TH-2 cells as a fusion protein including a 15-residue N-terminal leader from beta-galactosidase coded by the lacZ gene
-
expression in Escherichia coli, NADP-ME1
-
expression in Escherichia coli, NADP-ME2
-
expression in Escherichia coli, NADP-ME3
-
expression in Escherichia coli, NADP-ME4
-
expression in tobacco plants via infection with Agrobacterium tumefaciens
-
expression of a glutathione S-transferase fusion proteins NADP-ME2 in Escherichia coli
expression of a glutathione S-transferase fusion proteins NADP-ME3 in Escherichia coli
expression of His-tagged chimeric NADP-ME mutants in Escherichia coli strain BL21(DE3)
-
expression of His-tagged isozymes ME1 and ME2 in Escherichia coli strain BL21(DE3)
expression of isozyme NADP-ME2 in Escherichia coli strain BL21(DE3)
-
expression of the maize enzyme in Arabidopsis thaliana under control of the cauliflower mosaic virus 35S promoter
-
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
expression of wild-type and mutant isozymes NADP-ME2 in Escherichia coli
-
expression of wild-type and mutants in Escherichia coli BL21(DE3)
-
five NADP-ME genes PtNADP-ME1, PtNADP-ME2, PtNADP-ME3, PtNADP-ME4, and PtNADPME5, DNA and amino acid sequence determination and analysis, phylogenetic analysis, individual expression of GST-tagged isozymes in Escherichia coli
-
functional expression of ME1, under the control of cytomegalovirus, which also directs the transcription of GFP from an internal ribosome entry site, in Rattus norvegicus INS-1 832/13 cells and in Mus musculus islets
-
functional recombinant enzyme expression in Rhodotorula glutinis strain GM4, subcloning in Escherichia coli strain DH5alpha
gene hvme1, DNA and amino acid sequence determintion and analysis, isozyme expression analysis, hvme1 promoter analysis, expression of His-tagged isozyme Hvme1 in Escherichia coli strain BL21(DE3)
gene hvme2, DNA and amino acid sequence determintion and analysis, isozyme expression analysis, hvme1 promoter analysis
gene hvme3, DNA and amino acid sequence determintion and analysis, isozyme expression analysis, expression of His-tagged isozyme Hvme3 in Escherichia coli strain BL21(DE3)
gene maeB, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene maeB, overexpression in wild-type strain W3110, and in strains deficient for pyruvate formate lyase, lactate dehydrogenase, and glucose phosphotransferase, overview
-
gene maeB, sequence comparisons, recombinant expression in Escherichia coli strain BL21 resulting in increased malic enzyme activity and a 4fold enhancement in lipid content. Overexpressing the NADP-ME of Escherichia coli strain BL21 results in a 15% decrease in fatty acid production instead
gene malE, DNA and amino acid sequence determination and analysis, genomic library construction, screening, and functional enzyme expression in Escherichia coli
gene malE1, recombinant overexpression of the gene encoding ME isoform E from Mortierella alpina via Agrobacterium tumefaciens-mediated transformation in Mortierella alpina uracil auxotrophic strain increasing the fatty acid content by 30% compared to that for wild-type control, quantitative reverse transcription PCR enzyme expression analysis. The expression of the malE1 gene in three randomly selected malE1-overexpressing strains
gene Sco5261, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expressionin Escherichia coli strain BL21(DE3)
gene tme, subcloning in Escherichia coli, expression in bacteroids deficient for the NAD+-dependent malic enzyme encoded by gene dme, EC 1.1.1.39
-
genes NADP-ME1, NADP-ME2, and NADP-ME3, quantitative RT-PCR expression analysis
-
isozyme DNA and amino acid sequence determination and analysis, phylogenetic analysis, overview
isozyme NADP-ME2, DNA and amino acid sequence determination and analysis by screening of a root cDNA library, expressionin transgenic Arabidopsis thaliana plants using Agrobacterium tumefaciens strain EHA105-mediated transformation
isozyme NADP-ME2, expression in Escherichia coli
isozymes expression analysis, overview
-
ME gene, DNA and amino acid sequence determination and analysis, recombinant enzyme expression of the enzyme in Rhodotorula glutinis strain GM4 using the PGK1 promoter, a strong constitutive promoter of yeast PGK1 gene, co-expression with the PGK1 gene from Saccharomyces cerevisiae
ME1 and ME3, quantitative real time PCR expression analysis
-
mutant enzymes expressed in Escherichia coli
-
NADP-MDH gene, cDNA library screening, promoter analysis and DNA and amino acid sequence determination and analysis, genetic structure and sequence comparison with Brassicaceae plant sequences, overview, expression oof NADP-MDH-HIS3 fusion genes in the yeast two-hybrid system, Saccharomyces cerevisiae strain YM4271, identifying interaction with DNA binding proteins, overview
nadp-me1, expression in Escherichia coli
overexpression in Escherichia coli
-
overexpression of His-tagged wild-type and mutants in Escherichia coli strain DH5alpha, functional complementation of the enzyme deficient Escherichia coli triple mutant strain EJ1321
preparation of a genomic library, DNA and amino acid sequence determination and analysis, detailed phylogenetic reconstruction of malic enzymes, overview
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration
sequence comparisons
sequence comparisons, recombinant enzyme overexpression in Chlorella pyrenoidosa
-
sequence comparisons, recombinant expression in Mortierella alpine
sequence comparisons,, recombinant expressionin Mucor circinelloides
subcloning in strain K-12, expression of the His-tagged enzyme in strain BL21(DE3)
-