1.1.1.405: ribitol-5-phosphate 2-dehydrogenase (NADP+)

This is an abbreviated version!
For detailed information about ribitol-5-phosphate 2-dehydrogenase (NADP+), go to the full flat file.

Reaction

D-ribitol 5-phosphate
+
NADP+
=
D-ribulose 5-phosphate
+
NADPH
+
H+

Synonyms

ACS1, Bcs1, bifunctional ribulose 5-phosphate reductase/CDP-ribitol pyrophosphorylase, ribulose-5-P reductase, ribulose-5-phosphate reductase, TarIJ, TarJ

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.405 ribitol-5-phosphate 2-dehydrogenase (NADP+)

Cloned

Cloned on EC 1.1.1.405 - ribitol-5-phosphate 2-dehydrogenase (NADP+)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in ERscherichia coli
expression in Escherichia coli
overexpression of TarI and TarJ individually from their respective genes using the T7 expression system
-
spr1148 gene amplified, inserted into plasmid pJFK118EH and transformed into competent cells of Escherichia coli DH5alpha. Plasmid pJFK118EH1148 isolated and insert with the spr1148 gene cut off by restriction enzymes NdeI and SacI. Fragment ligated into the overexpression plasmid pET28a(+) to generate plasmid pET1148. The plasmid transformed into Escherichia coli BL21(DE3) for overexpression of spr1148