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1.1.1.44: phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating)

This is an abbreviated version!
For detailed information about phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating), go to the full flat file.

Word Map on EC 1.1.1.44

Reaction

6-phospho-D-gluconate
+
NADP+
=
D-ribulose 5-phosphate
+
CO2
+
NADPH
+
H+

Synonyms

6-GPD, 6-P-gluconate dehydrogenase, 6-Pgd, 6-PGDH, 6-PGDHase, 6-phospho-D-gluconate dehydrogenase, 6-phospho-D-gluconate-NADP+ oxidoreductase, decarboxylating, 6-phosphogluconate dehydrogenase, 6-phosphogluconate dehydrogenase (decarboxylating), 6-phosphogluconate dehydrogenase 1, 6-phosphogluconate dehydrogenase Gnd1, 6-phosphogluconate dehydrogenase, decarboxylating, 6-phosphogluconate-dehydrogenase, 6-phosphogluconate:NADP oxidoreductase, 6-phosphogluconic carboxylase, 6-phosphogluconic dehydrogenase, 6-phosphonogluconate dehydrogenase, 6PDH, 6PG DH, 6PGD, 6PGDH, 6PGDH/Gnd1, 6PGDH1, 6PGDH2, 6PGDH3, At1G64190, At3g02360, At5g41670, D-gluconate-6-phosphate dehydrogenase, GCG1, gnd, GND1, Gnd1p, Gnd2p, GNTZII, LlPDH, Moth_1283, Os6PGDH1, Os6PGDH2, p6PGDH, peroxisomal 6-phosphogluconate dehydrogenase, Pgd, PGD1, PGD2, PGD3, phosphogluconic acid dehydrogenase, TM0438, YpjI, zwf3

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.44 phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating)

Purification

Purification on EC 1.1.1.44 - phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2',5'-ADP-Sepharose 4B column chromatography
-
725.4fold from erythrocytes by ammonium sulfate precipitation, 2',5'-ADP-resin affinity chromatography, and gel filtration
-
78.5fold, partial from erythrocytes by ammonium sulfate precipitation, fraction 60-80% saturation
-
ammonium sulfate precipitation, 2',5'-ADP Sepharose 4B affinity chromatography and Sephadex G200 gel filtration
-
HisPur Ni-NTA resin column chromatography
HisTrap column chromatography and Superdex 200 gel filtration
-
native enzyme by partially 27fold ion exchange, affinity and size exclusion chromatography, His-tagged recombinant wild-type and mutant enzymes by nickel affinity chromatography
Ni-NTA column chromatography
Ni-NTA column chromatography and ADP-agarose column chromatography
-
Ni-NTA resin chromatography and ADP-agarose affinity column chromatography
Ni-NTA resin column chromatography and 2?5?ADP agarose chromatography
Ni2+-NTA affinity chromatography
-
Ni2+-NTA column chromatography
partially, fractionation
-
PEG precipitation, Cibacron Blue column chromatography, ADP agarose column chromatography, and phenyl Sepharose column chromatography
-
purified from an overexpressing Escherichia coli strain
-
recombinant enzyme
-
recombinant from Escherichia coli
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity
Sephadex G-25 column chromatography, DEAE-Sepharose Fast Flow ion exchange chromatography and 2',5'-ADP Sepharose 4B column chromatography
-
wild-type, mutant col-3, mutant col-10
-