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(NH4)2SO4
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1 M, 71% inhibition
2-mercaptoethanol
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1 mM, room temperature, pH 9.0, rapid inactivation
3-(2-bromoacetyl)pyridine
active-site-directed irreverseible inhibitor, the cofactor NAD but not the substrate glucose protects the enzyme from the inactivation, in the presence of 0.1 M NAD and 1.37 mM 3-(2-bromoacetyl)pyridine, glucose dehydrogenase has still 85% of its original activity after incubation for 2 h
5,5'-dithiobis(2-nitrobenzoic acid)
-
1 mM, room temperature, pH 9.0, 50% inactivation
acetone
-
1% residual activity at 50% (v/v)
Ag+
-
1 mM, 99% inhibition
Cd2+
more than 50% inhibition at 20 mM
D-glucono-1,5-lactone
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forward reaction, oxidation, product inhibition, noncompetitive vs. beta-D-glucose and NADP+
dithiothreitol
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10 mM, 28% inhibition
ethanol
-
19% residual activity at 50% (v/v)
KCl
-
1 M, 44% inhibition
LiCl
-
1 M, 64% inhibition
n-butanol
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1% residual activity at 50% (v/v)
N-ethylmaleimide
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3 mM, room temperature, pH 9.0, 50% inactivation
NADH
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0.1 mM, 45% inhibition, noncompetitive inhibitorr of D-glucose
p-chloromercuribenzoate
-
1 mM, 93% inhibition
Tetranitromethane
the rapid inactivation can be prevented by the presence of NAD, AMP, or ATP
thioredoxin
-
in the presence of 0.5 mM dithiothreitol
Ca2+
-
1 mM, 14% inhibition
Ca2+
60% inhibition at 50, 100 and 10 mM in sodium phosphate buffer at pH 8.0, Tris/HCl buffer at pH 8.0 and Tris/HCl buffer at pH 9.0, respectively
Ca2+
-
1 mM, 33% inhibition
Co2+
-
1 mM, 74% inhibition
Co2+
more than 50% inhibition at 20 mM
Co2+
-
1 mM, 44% inhibition
Cu2+
-
1 mM, 14% inhibition
Cu2+
more than 50% inhibition at 20 mM
Cu2+
-
1 mM, 83% inhibition
EDTA
-
50 mM, 5% activity left
EDTA
60% inhibition at 10 mM
EDTA
-
1 mM, 68% inhibition, fully reversible by 20 mM MgCl2
Fe2+
-
1 mM, 41% inhibition
Fe2+
complete inhibition at 10 mM
Fe3+
-
1 mM, 41% inhibition
Fe3+
complete inhibition at 10 mM
Hg2+
-
1 mM, complete inhibition
Hg2+
-
1 mM, 85% inhibition
Mn2+
-
1 mM, 16% inhibition
Mn2+
more than 50% inhibition at 20 mM
Mn2+
-
1 mM, 41% inhibition
NaCl
-
1 M, 28% inhibition
NaCl
-
reaction rate and dissociation at pH 9 are reduced by increasing the NaCl concentration (0-500 mM)
NADP+
-
3 mM, 30% inhibition
NADP+
-
reverse reaction, reduction, product inhibition, competitive vs. NADPH and noncompetitive vs. D-glucono-1-5-lactone
NADPH
-
0.1 mM, 19% inhibition
NADPH
-
product inhibition, competitive with respect to NADP
Ni2+
-
-
Ni2+
-
1 mM, 85% inhibition
PCMB
-
0.1 mM, complete inhibition
Zn2+
-
1 mM, 8% inhibition
Zn2+
complete inhibition at 10 mM
Zn2+
-
1 mM, 25% inhibition
additional information
no inhibition by EDTA and thiol reagents such as fluorescein 5-maleimide
-
additional information
-
hexadecyltrimethylammonium bromide, i.e. CTAB, does not influence the enzyme activity but increases the enzyme stability
-