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1.1.1.86: ketol-acid reductoisomerase (NADP+)

This is an abbreviated version!
For detailed information about ketol-acid reductoisomerase (NADP+), go to the full flat file.

Word Map on EC 1.1.1.86

Reaction

(2R)-2,3-dihydroxy-3-methylbutanoate
+
NADP+
=
(2S)-2-hydroxy-2-methyl-3-oxobutanoate
 +
NADPH
 +
H+

Synonyms

2-hydroxy-3-keto acid reductoisomerase, acetohydroxy acid isomeroreductase, acetohydroxy acid reductoisomerase, acetohydroxy-acid isomeroreductase , acetohydroxy-acid reductoisomerase , acetohydroxyacid isomeroreductase, acetolactate reductoisomerase, AHAIR, AHIR, alpha-keto-beta-hydroxylacil reductoisomerase, alpha-keto-beta-hydroxylacyl reductoisomerase, class II ketol-acid reductoisomerase, dehydrogenase, dihydroxyisovalerate (isomerizing), dihydroxyisovalerate dehydrogenase (isomerizing), EC 1.1.1.89, Icl1p, Ilv5p, ilvC, IlvC-PanE, IlvC1, IlvC2, isomerase, ketol acid reducto-, isomeroreductase, KARI, ketol-acid reductoisomerase, reductoisomerase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.86 ketol-acid reductoisomerase (NADP+)

Specific Activity

Specific Activity on EC 1.1.1.86 - ketol-acid reductoisomerase (NADP+)

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.4
substrate: hydroxypyruvate, pH 8.0, 25°C
0.5
-
substrate: hydroxypyruvate, pH 8.0, 25°C
1.97
purified recombinant enzyme, substrate hydroxypyruvate
3
-
deletion mutant DELTA423-430/F431S
6
substrate: (2S)-2-acetolactate, pH 8.0, 25°C
additional information
-
the specific activity with pyruvate is 1% and with 2-ketovalerate, 2-ketopantoate and 2-ketobutyrate is 8% of that of 2-acetolactate, comparison of activities of wild-type and mutant enzymes