1.1.1.87: homoisocitrate dehydrogenase

This is an abbreviated version!
For detailed information about homoisocitrate dehydrogenase, go to the full flat file.

Word Map on EC 1.1.1.87

Reaction

(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate
+
NAD+
=
2-oxoadipate
+
CO2
+
NADH
+
H+

Synonyms

(-)-1-hydroxy-1,2,4-butanetricarboxylate:NAD+ oxidoreductase (decarboxylating), 2-hydroxy-3-carboxyadipate dehydrogenase, 3-carboxy-2-hydroxyadipate dehydrogenase, 3-carboxy-2-hydroxyadipate:NAD+ oxidoreductase (decarboxylating), dehydrogenase, homoisocitrate, EC 1.1.1.155, HIc, HIc dehydrogenase, HICDH, homoisocitrate dehydrogenase, homoisocitric dehydrogenase, isocitrate-homoisocitrate dehydrogenase, LYS12, protein PH1722, ScHICDH

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.87 homoisocitrate dehydrogenase

Cloned

Cloned on EC 1.1.1.87 - homoisocitrate dehydrogenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli
DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain OM17
-
expressed in Escherichia coli BL21(DE3)
-
expression in Escherichia coli
-
expression in Escherichia coli. Introduction of homocitrate synthase, homoaconitase and homoisocitrate dehydrogenase from Saccharomyces cerevisiae into Escherichia coli enables +1 carbon extension from 2-oxoglutarate to 2-oxoadipate, which is subsequently converted into glutarate by a promiscuous 2-oxo acid decarboxylase (KivD) and a succinate semialdehyde dehydrogenase (GabD)
-
expression of His-tagged enzyme in Escherichia coli strain TOP 10F'
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LYS1 gene codes for the alpha-aminoadipate pathway enzymes in fungi, can complement LYS1-deficient mutants of Candida albicans and Saccharomyces cerevisiae, amplification in Escherichia coli
-
recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-