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enzyme in binary complex with inhibitor (2S,3S)-thiahomoisocitrate, sitting drop vapour diffusion method, 0.00 ml of 8.7 mg/ml protein in 5 mM Tris-HCl, pH 7.8. 0.8 mM inhibitor, and 1.7 mM NAD, is mixed with 0.002 ml of reservoir solution containing 40% 2-methyl-2,4-pentandiol and 100 mM citrate pH 4.85, eqilibration against 1 ml resetvoir solution, X-ray diffrcation structure determination and analysis at 2.6 A resolution, molecular replacement
purified recombinant enzyme, hanging drop vapour diffusion method, 10 mg/ml protein with reservoir solution containing 24% PEG 400, and 0.1 M citrate, pH 4.8, addition of 0.003 ml drops of 0.1 M MgCl2, 5 mM isocitrate or homoisocitrate, and of a 0.001 ml drop 50 mM CdCl2, equilibration against 0.5 ml reservoir solution, 5 days at 20°C, X-ray diffraction structure determination and analysis at 1.85 A resolution, molecular replacement