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1.1.1.9: D-xylulose reductase

This is an abbreviated version!
For detailed information about D-xylulose reductase, go to the full flat file.

Word Map on EC 1.1.1.9

Reaction

xylitol
+
NAD+
=
D-xylulose
 +
NADH
 +
H+

Synonyms

2,3-cis-polyol(DPN) dehydrogenase (C3-5), D-xylulose reductase A, erythritol dehydrogenase, GmXDH, IoXyl2p, McXDH, More, NAD+-dependent XDH, NAD+-dependent xylitol dehydrogenase, NAD+-linked xylitol dehydrogenase, NAD-dependent xylitol dehydrogenase, NADH-dependent XDH, NADH-dependent xylitol dehydrogenase, nicotinamide adenine dinucleotide-dependent xylitol dehydrogenase 2, pentitol-DPN dehydrogenase, Ps-XDH, PsXDH, reductase, D-xylulose, RpXDH, slSDH, SpXYL2.2, SsXyl2p, TdXyl2p, XDH, XDH-Y25, xdhA, XL2, XYL2, XYL2.1, XYL2.2, xylitol dehydrogenase, xylitol dehydrogenase 2, xylitol-2-dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.9 D-xylulose reductase

Cloned

Cloned on EC 1.1.1.9 - D-xylulose reductase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed as a His-tagged fusion protein in Escherichia coli
-
expressed in Candida glycerinogenes
-
expressed in Candida glycerinogenes strain WL2002-5
expressed in Escherichia coli
-
expressed in Escherichia coli BL21 cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli JM 109
-
expressed in Saccharomyces cerevisiae strain DX23
expressed in transketolase-deficient Saccharomyces cerevisiae strain (W303/tkl1 tkl2/2C)
-
expression in Escherichia coli
expression of the engineered D202A/L203R/V204S/E205P/S206R mutant enzyme fron Galactocandida mastotermitis with altered cofactor specificity, co-expression with a mutant NADPH-specific xylulose reductase from Candida tenuis in Saccharomyces cerevisiae, the transformed strain shows up to 50% decreased glycerol yield without increase in ethanol during xylose fermentation, overview
-
gene SpXYL2.2, recombinant expression of the enzyme in Saccharomyces cerevisiae strain CEN.PK2-1C (MATa leu2-3112 ura3-52 trp1-289 his3-DELTA1 MAL2-8c SUC2), subcloning in Escherichia coli strain DH5alpha, co-expression with xylose reductase from Spathaspora arborariae
-
gene xdh, cloning from strain CGMCC 1.49, DNA and amino acid sequence determination and analysis, functional recombinant overexpression of His6-tagged enzyme in Escherichia coli strain BL21
gene xdhA
gene xdhA or xyl2
gene xdhA, coexpression of gene xdhA and cofactor regeneration enzyme, i.e. glucose dehydrogenase gene gdh from Bacillus subtilis, in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain JM109
-
gene xyl2, DNA and amino acid sequence determination and analysis, cloning in Escherichia coli strain JM109, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene Xyl2, DNA and amino acid sequence determination and analysis, sequence comparisons
gene xyl2, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene xyl2, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene xyl2, overexpression in Gluconobacter strain PXPG, coexpression with the glucose dehydrogenase gene from Bacillus subtilis
gene XYL2, recombinant expression in Saccharomyces cerevisiae strain SCB7 resulting in strains SCF202 and SCF201, coexpression of XYL2 with XYL1, encoding a xylose reductase, and the endogenous XKS1, encoding a xylulokinase, all under the control of the TDH3 promoter in the chromosomal DNA, the recombinant strain efficiently grows in minimal medium containing xylose as the sole carbon source. An additional mutations of MTH1, encoding the endogenous negative regulator of the glucose-sensing signal transduction pathway, MTH1e32, and GRR1, encoding endogenous Grr1, an Fbox protein component of an SCF ubiquitineligase complex grr1e33, enable efficient growth in medium containing high xylose concentrations, subcloning in Escherichia coli strain DH10B
-
gene xyl2, recombinant expression of the enzyme in Synechococcus elongatus strain PCC7942, functional co-expression with a xylose transporter from Escherichia coli, Ec-XylE
gene xyl2, the xylose-metabolic gene XYL2 from Scheffersomyces stipitis is introduced into Saccharomyces cerevisiae
gene XYL2.1, recombinant expression in Aureobasidium pullulans strain CBS 110374
genes XYL2 (D207A/I208R/F209S) and XYL2 (S96C/S99C/Y102C/D207A/I208R/F209S) are introduced into Saccharomyces cerevisiae, which already contain the Pichia stipitis XYL1 gene (encoding xylose reductase) and the endogenously overexpressed XKS1 gene (encoding xylulokinase)
-
overexpression in Saccharomyces cerevisiae
-
overexpression of the enzyme in Saccharomyces cerevisiae strain CEN.PK 113-7D under control of the constitutive TDH3 promoter, co-expression with xylose reductase from Candida tenuis
-
recombinant enzyme expression in Saccharomyces cerevisiae strain CA11 (isolated from Brazilian Cachaca fermentation processes) and in strain PE-2DELTAGRE3 (isolated from Brazilian first generation bioethanol plants), coepxression with xylose reductase (XR)
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
recombinant overexpression in Saccharomyces cerevisiae strain D452-2, coexpression with Saccharomyces cerevisiae enzymes/genes xylulokinase XKS1, and xylose reductase XYL1 or XYL3, aldose reductase GRE3. Xylose fermentation efficiency of a strain overexpressing GRE3 is dramatically increased by high expression levels of XYL2