1.1.2.3: L-lactate dehydrogenase (cytochrome)

This is an abbreviated version!
For detailed information about L-lactate dehydrogenase (cytochrome), go to the full flat file.

Word Map on EC 1.1.2.3

Reaction

(S)-lactate
+ 2 ferricytochrome c =
pyruvate
+ 2 ferrocytochrome c + 2 H+

Synonyms

Cyb2, cytochrome b2, cytochrome c oxido reductase, FC b2, Fcb2, flavocytochrome b, flavocytochrome b2, iLDH, L(+)-lactate:cytochrome c oxidoreductase, L-(+)-lactate ferricytochrome c oxidoreductase, L-lactate cytochrome c oxidoreductase, L-lactate cytochrome c reductase, L-lactate dehydrogenase [Cytochrome], L-lactate ferricytochrome C oxidoreductase, L-lactate-cytochrome c oxidoreductase, L-lactate-cytochrome c-oxidoreductase, L-lactate: cytochrome c oxidoreductase, L-lactate:cytochrome c oxidoreductase, L-lactate:cytochrome c-oxidoreductase, L-LCR, L-LDH (FMN-dependent), lactate dehydrogenase (cytochrome), lactic acid dehydrogenase, lactic cytochrome c reductase, NAD+ - independent LDH

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.2 With a cytochrome as acceptor
                1.1.2.3 L-lactate dehydrogenase (cytochrome)

Storage Stability

Storage Stability on EC 1.1.2.3 - L-lactate dehydrogenase (cytochrome)

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-180°C, long term storage
-
-70°C in 0.1 M phosphate buffer, 1 mM EDTA, pH 7, 10 mM DL-lactate is added to the Fcb2 preparations to keep the enzyme in the reduced state
-
-70°C, in 0.1 M phosphate buffer, 1 mM EDTA, pH 7, 10 mM DL-lactate is added to the Fcb2 preparations to keep the enzyme in the reduced state
-
-70°C, purified enzymes are stored in 100 mM potassium phosphate, 1 mM EDTA, and 20 mM D,L-lactate at pH 7.5
-
-80°C as saturated solution
-
-80°C, 0.1 M phosphate buffer, 1 mM EDTA, 1 mM PMSF
-
20°C, 0.2 M phosphate buffer, pH 7.2, 1 mM EDTA, protected from light, 50% loss of activity within 10-20 days
-
4°C, 70% saturated ammonium sulfate, under nitrogen, 2 months, 20% loss of activity of purified enzyme
-
4°C, precipitate from 70% saturated ammonium sulfate, under nitrogen, stable for several weeks
-
4°C, saturated ammonium sulfate, under nitrogen, protected from light, stabe
-
under nitrogen, 1 month, no loss of activity
-