1.1.3.12: pyridoxine 4-oxidase
This is an abbreviated version!
For detailed information about pyridoxine 4-oxidase, go to the full flat file.
Word Map on EC 1.1.3.12
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1.1.3.12
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pyridoxal
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mesorhizobium
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loti
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fad
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synthesis
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chaperonins
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nitrogen-fixing
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luteolum
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microbacterium
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4-dehydrogenase
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symbiotic
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pyridoxamine
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pyridoxamine-pyruvate
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hydride
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aminotransferase
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glucose-methanol-choline
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fad-dependent
- 1.1.3.12
- pyridoxal
-
mesorhizobium
- loti
- fad
- synthesis
- chaperonins
-
nitrogen-fixing
- luteolum
-
microbacterium
-
4-dehydrogenase
-
symbiotic
- pyridoxamine
-
pyridoxamine-pyruvate
-
hydride
- aminotransferase
-
glucose-methanol-choline
-
fad-dependent
Reaction
Synonyms
oxidase, pyridoxol 4-, PN 4-oxidase, PNO, PNOX, pyridoxin 4-oxidase, pyridoxine-4-oxidase, pyridoxol 4-oxidase
ECTree
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Crystallization
Crystallization on EC 1.1.3.12 - pyridoxine 4-oxidase
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purified recombinant C-terminally His6-tagged PNOX and PNOX-pyridoxamine complex, sitting drop vapour diffusion mthod and micro-seeding method, mixing of 0.002 ml of 10 mg/ml PNOX with 0.002 ml of a precipitant solution comtaining 0.1 M HEPES-NaOH, pH 7.5, 10% v/v 2-propanol, and 20% w/v PEG 4000, equilibration against 01 ml of precipitant solution, 4°C, primary crystals are crushed and suspended in 0.5 ml of a solution consisting of equal volumes of a crystallization buffer containing 50 mM Tris-HCl, pH 8.0, 10% w/v glycerol, 0.005 mM FAD, 0.1% v/v 2-mercaptoethanol, and 0.01% v/v Tween 20, and precipitant solution as seeding solution, or seeding crystals are prepared by vapor diffusion in a mixture consisting of 0.002 ml of 13 mg/ml PNOX, and 0.002 ml of the precipitant solution comprising 0.1 M HEPES-NaOH, pH 7.5, 25% v/v 2-propanol, 20% w/v PEG 4000, and 10 mM pyridoxamine, 3 weeks, X-ray diffraction structuure determination and analysis at 2.2 A and 2.1 A resolutions, respectively, molecular replacement