1.1.3.21: glycerol-3-phosphate oxidase

This is an abbreviated version!
For detailed information about glycerol-3-phosphate oxidase, go to the full flat file.

Word Map on EC 1.1.3.21

Reaction

sn-glycerol 3-phosphate
+
O2
=
glycerone phosphate
+
H2O2

Synonyms

alpha-glycerol-3-phosphate oxidase, alpha-glycerophosphate oxidase, G2POx, GLPD, GlpO, GlpO protein, glycerol phosphate oxidase, glycerol-1-phosphate oxidase, glycerol-3-P oxidase, Glycerol-3-phosphate oxidase, GP oxidase, GPO, L-alpha-glycerol-3-phosphate oxidase, L-alpha-glycerophosphate oxidase, oxidase, glycerol phosphate

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.21 glycerol-3-phosphate oxidase

Storage Stability

Storage Stability on EC 1.1.3.21 - glycerol-3-phosphate oxidase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 40 d, 13% loss of activity, 4°C, 14 d, 92% loss of activity, 30°, 24 h, 90% loss of activity, 40°C, 80 min, 80% loss of activity, 4°C, 0.2% dithiothreitol, 20 d, 32% loss of activity, 4°C, 0.2% mercaptoethanol, 20 d, 34% loss of activity, 4°C, 50% glycerol, 20 d, 36% loss of activity, 4°C, 100 mM EDTA, 20 d, 24% loss of activity, 4°C, 2.5% polyethylenglycol, 20 d, 63% loss of activity, 4°C, lyophilization, 2% dextrin II 14 d, 35% loss of activity, 20°C, lyophilization, 2% dextrin II, 14 d, 60% loss of activity, 37°C, lyophilization, 2% dextrin II, 14 d, 85% loss of activity
-
2°C, several weeks, slow decline of activity
-
40°C, during storage for 21 days 20% of activity lost, sodium azide as stabilizer in a concentration of 0.05% maintains activity of the enzyme in 98.4%, addition of sucrose maintains only 74% of activity
-
immobilized enzyme, 50% loss of activity after 150 uses in analysis for triglycerides, within 33 days at 4°C in 0.1 M sodium phosphate buffer, pH 7.0
-