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1.1.3.6: cholesterol oxidase

This is an abbreviated version!
For detailed information about cholesterol oxidase, go to the full flat file.

Word Map on EC 1.1.3.6

Reaction

cholesterol
+
O2
=
cholest-5-en-3-one
 +
H2O2

Synonyms

3beta-hydroxy steroid oxidoreductase, 3beta-hydroxysteroid: oxygen oxidoreductase, 3beta-hydroxysteroid:oxygen oxidoreductase, 3beta-hydroxysterol oxidase, BsChOx, CgChoA, CHO, CHO-U, ChO3, ChoA, choBb, CHOD, ChoG, ChoL, cholesterol oxidase, cholesterol oxidase I, cholesterol oxidase II, cholesterol-O2 oxidoreductase, CHOLOX, choM, ChoM1, ChoM2, choP, ChoRI, ChoRII, ChoS, ChOx, CO, CO1, COase, COD, COD-B, COX, HCEO-forming enzyme, HMPREF0204_11499, oxidase, cholesterol, PimE, ShChOx, type I ChOx

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.6 cholesterol oxidase

Activating Compound

Activating Compound on EC 1.1.3.6 - cholesterol oxidase

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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
acetone
-
3.8fold increase in activity, 1 ml solvent added to 2 ml 50 mM phosphate buffer
Benzene
Bile salts
-
-
chloroform
-
7% activation at 0.5% w/v
cholic acid
-
stimulates without a significant change in Km in the presence of low Triton X-100 concentrations, changes the sigmoidal kinetic into normal Michaelis-Menten kinetic with reduced Km in the presence of high Triton X-100 concentrations
diphenylmethane
-
strain ST-200, 3.1fold increase in activity, 1 ml solvent added to 2 ml 50 mM phosphate buffer, pH 7.0
dodecylpoly(ethylene glycol ether)9-10
-
ethanol
Isopropanol
Long-chain alcohols
-
maximum activation with decanol and undecanol, shorter and longer alcohols are less effective
methanol
-
activates 1.15fold at 10%
NaN3
-
activates slightly
p-xylene
-
strain ST-200, 3.4fold increase in activity, 1 ml solvent added to 2 ml 50 mM phosphate buffer, pH 7.0
phosphate ions
propan-2-ol
propylbenzene
-
strain ST-200, 3.2fold increase in activity, 1 ml solvent added to 2 ml 50 mM phosphate buffer, pH 7.0
Sarcosyl
sodium cholate
Toluene
Triton X-100
Triton X-114
-
acivates 2fold at 0.5%
Triton X-405
Tween 20
Tween 80
-
acivates 1.5fold at 0.5%
Tween-80
-
18% activation at 0.5% w/v
additional information
-
the bacterial COX exhibits 22 % higher activity in potassium phosphate buffer, pH 7.5, in the presence of a nonionic detergent Triton X-100 at 0.05 % v/v
-