1.1.3.9: galactose oxidase

This is an abbreviated version!
For detailed information about galactose oxidase, go to the full flat file.

Word Map on EC 1.1.3.9

Reaction

D-galactose
+
O2
=
D-galacto-hexodialdose
+
H2O2

Synonyms

AOd, beta-galactose oxidase, D-galactose oxidase, FgrGalOx, galactose 6-oxidase, galactose oxidase, GalOx, GAO, GAOA, GAOX, GO, GOase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.9 galactose oxidase

Cloned

Cloned on EC 1.1.3.9 - galactose oxidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
computational active-site models for galactose oxidase for the distinctive oxidation states (Cu2+ protein complex/radical), additional models including solvent molecules and second coordination sphere residues
-
expressed in Escherichia coli BL21 cells
-
expression in Aspergillus nidulans
-
expression in Aspergillus oryzae, Fusarium venenatum
-
expression in Pichia pastoris
-
functional expression of wild-type and mutant enzymes in Escherichia coli strain BL21 Star (DE3) cytoplasm, best in strain BL21 Star (DE3) at 25°C, induction for 4-6 h is optimal
-
gene gao, recombinant expression in in Aspergillus nidulans, Pichia pastoris, and Escherichia coli
gene gao, recombinant expression of wild-type enzyme and chimeric enzyme mutants GaO-CBM29 and CBM29-GaO in Pichia pastoris strain KM71H
gene gaoA, DNA and amino acid sequence determination and analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene GAOA, phylogenetic tree
gene GAOA, sequence comparison to the Fusarium graminearum enzyne, sucessful recombinant expression of C-terminally His-tagged enzyme in Escherichia coli even without codon optimization or further amino acid substitutions. The C-terminal His-tag added to recombinant GalOx from Fusarium oxysporum does not interfere with the catalytic activity. Sucessful recombinant expression of C-terminally His-tagged full-length enzyme and enzyme with the alpha-factor signal sequence of Saccharomyces cerevisiae in Pichia pastoris
high level expression in Pichia pastoris
-
recombinant expression of C-terminally His-tagged enzyme in Pichia pastoris Mut+ strain PCE, under regulation of the AOX1 promoter, low exponential methanol feeding leads to 1.5fold higher volumetric productivity compared to high exponential feeding rates, culture condition and methanol feeding optimization, overview
recombinant expression of galactose oxidase in Pichia pastoris
-
recombinant expression of galactose oxidase without the pro sequence. Reaction with excess Cu2+ leads to formation of the C228-Y272 thioether bond both under aerobic and anaerobic conditions, the formation of the Y272 radical occures solely under aerobic conditions (posttranslational modifications)