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1.1.3.9: galactose oxidase

This is an abbreviated version!
For detailed information about galactose oxidase, go to the full flat file.

Word Map on EC 1.1.3.9

Reaction

D-galactose
+
O2
=
D-galacto-hexodialdose
 +
H2O2

Synonyms

AOd, At1g14430, At1g19900, At1g67290, At1g75620, At3g53950, At3g57620, At5g19580, beta-galactose oxidase, D-galactose oxidase, F5K20_250, FgrGalOx, galactose 6-oxidase, galactose oxidase, GalOx, GAO, GAOA, GAOX, GLOX1, Glox2, Glox3, GLOX4, GLOX5, GLOX6, GO, GOase, RUBY, RUBY PARTICLES IN MUCILAGE

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.3 With oxygen as acceptor
                1.1.3.9 galactose oxidase

Purification

Purification on EC 1.1.3.9 - galactose oxidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
covalently immobilized to chemically modified porous silica particles
-
Ni-charged resin column chromatography
-
purification of commercially available GOase mutant M3-5
-
purification of recombinant galactose oxidase under strict copper-free conditions
purification using immobilized biomimetic ligands or D(+)-galactosamine, two-step purification procedure using immobilized 1-amino-1-deoxy-beta-D-galactose
-
recombinant C-terminally His-tagged enzyme 28fold from Escherichia coli, recombinant C-terminally His-tagged full-length enzyme (including the native prepro signal sequence) 6fold from Pichia, and recombinant C-terminally His-tagged enzyme with alpha-factor signal sequence 22fold from Pichia pastoris
recombinant C-terminally His6-tagged enzyme 204fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant enzyme
-
recombinant wild-type enzyme and chimeric enzyme mutants GaO-CBM29 and CBM29-GaO from Pichia pastoris strain KM71H by nickel affinity and hydrophobic interaction chromatography or vice-versa, follwed by dialysis and anion exchange chromatography
the commercially availbale enzyme is further purified by gel filtration
the enzyme from commercial preparation is further purified by gel filtration
-
to homogeneity, 1step chromatography
-
to homogeneity, 1step chromoatography
Polyporus circinatus
-
to homogeneity, affinity chromatography
-
to homogeneity, chromatography techniques
to homogeneity, intra- and extracellular forms, 1step chromatography
-