1.1.99.1: choline dehydrogenase

This is an abbreviated version!
For detailed information about choline dehydrogenase, go to the full flat file.

Reaction

Synonyms

Cdh, CHD, CHDH, ChoDH, choline dehydrogenase, choline oxidase, choline-cytochrome c reductase, choline:(acceptor) oxidoreductase, dehydrogenase, choline, oxidase, choline

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.99 With unknown physiological acceptors

                1.1.99.1 choline dehydrogenase

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Results	in table
3	Activating Compound
12267	AA Sequence
3	Application
1	CAS Registry Number
9	Cloned(Commentary)
12	Cofactor
53	Disease/ Diagnostics
1	Expression
18	General Information
6	General Stability
28	Inhibitors
2	Ki Value [mM]
11	KM Value [mM]
18	Localization
1	Metals/Ions
6	Molecular Weight [Da]
10	Natural Substrates/ Products (Substrates)
33	Organism
6	Pathway
6	pH Optimum
2	pH Range
2	pH Stability
1	pI Value
1	Posttranslational Modification
3	Protein Variants
8	Purification (Commentary)
3	Reaction
3	Reaction Type
29	Reference
31	Source Tissue
7	Specific Activity [micromol/min/mg]
7	Storage Stability
66	Substrates and Products (Substrate)
9	Subunits
21	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
5	Temperature Stability [°C]

Purification

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Purification on EC 1.1.99.1 - choline dehydrogenase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

from rat liver mitochondria, purification methods, overview

Rattus norvegicus

Q6UPE0

741756

partial

Halomonas elongata

-

654268

partial, using Triton-treatment, chloroform-treatment and column chromatography on Sepharose 6B

Pseudomonas aeruginosa

-

389887

recombinant enzyme from Escherichia coli

Homo sapiens

Q8NE62

741756

recombnant enzyme from Escherichia coli strain M15 by ammonium sulfate fractionation and anion exchange chromatography

Escherichia coli

A7LLT8

725104

using 0.5% Brij 58 treatment, column chromatography on DEAE-cellulose and gel filtration on Toyopearl HW-60S

Canis lupus familiaris

-

389883

using column chromatography of the chemically modified enzyme with 5,5'-dithiobis(2-nitrobenzoic acid) on DEAE-Sepharose CL-6B and choline-Sepharose 4B with C3-spacer, subsequent release of the thionitrobenzoate with dithiothreitol, and a second column chromatography on DEAE-Sepharose CL-6B in the presence of 0.1% Triton X-100

Rattus norvegicus

-

389886

using column chromatography on thio-hexyl and on an anion exchanger

Pseudomonas aeruginosa

-

389890