1.1.99.18: cellobiose dehydrogenase (acceptor)
This is an abbreviated version!
For detailed information about cellobiose dehydrogenase (acceptor), go to the full flat file.
Word Map on EC 1.1.99.18
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1.1.99.18
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cellulose
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phanerochaete
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chrysosporium
-
biofuels
-
basidiomycete
-
flavocytochrome
-
lignocellulose
-
white-rot
-
laccase
-
pyranose
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corynascus
-
lpmos
-
myriococcum
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wood-degrading
-
trametes
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cellodextrins
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thermophilum
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ligninolytic
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cello-oligosaccharides
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cellulose-binding
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glucose-methanol-choline
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cellulose-degrading
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flavodehydrogenase
-
analysis
-
sporotrichum
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rolfsii
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insolens
-
myrothecium
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cellulose-grown
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biofuel production
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biotechnology
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industry
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dichlorophenol-indophenol
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brown-rot
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pycnoporus
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cellulose-based
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humicola
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wood-rotting
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bioanode
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verrucaria
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cellobiohydrolases
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diagnostics
-
medicine
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synthesis
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degradation
- 1.1.99.18
- cellulose
- phanerochaete
- chrysosporium
-
biofuels
-
basidiomycete
-
flavocytochrome
- lignocellulose
-
white-rot
- laccase
- pyranose
- corynascus
-
lpmos
-
myriococcum
-
wood-degrading
- trametes
- cellodextrins
- thermophilum
-
ligninolytic
- cello-oligosaccharides
-
cellulose-binding
-
glucose-methanol-choline
-
cellulose-degrading
-
flavodehydrogenase
- analysis
- sporotrichum
- rolfsii
- insolens
-
myrothecium
-
cellulose-grown
- biofuel production
- biotechnology
- industry
- dichlorophenol-indophenol
-
brown-rot
- pycnoporus
-
cellulose-based
- humicola
-
wood-rotting
-
bioanode
- verrucaria
- cellobiohydrolases
- diagnostics
- medicine
- synthesis
- degradation
Reaction
Synonyms
cbdA, CBO, CBOR, Cdh, CDH IIA, CDH IIB, cdh-1, CDH1, Cdh2, CDHIIA, cellobiose (acceptor) 1-oxidoreductase, cellobiose dehydrogenase, cellobiose dehydrogenase IIA, cellobiose oxidase, cellobiose oxidoreductase, cellobiose [acceptor] 1-oxidoreductase, Cellobiose-quinone oxidoreductase, cellobiose:(acceptor) 1-oxidoreductase, cellobiose:quinone oxidoreductase, cellobiose:[acceptor] 1-oxidoreductase, DCHsr, dehydrogenase, cellobiose, EC 1.1.3.25, EC 1.1.5.1, MtCDH, oxidase, cellobiose, Thite_59724, TpCDH, TvCDH
ECTree
1.1.99.18 cellobiose dehydrogenase (acceptor)Advanced search results
results (
results (KM Value
KM Value on EC 1.1.99.18 - cellobiose dehydrogenase (acceptor)
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.72
6'-azido-6'-deoxy-beta-D-glucopyranosyl-(1->4)-D-glucopyranose
-
at pH 4.5 and 30°C
1.7
beta-D-glucopyranosyl-(1->4)-2-azido-2-deoxy-D-glucopyranose
-
at pH 4.5 and 30°C
1.77
beta-D-glucopyranosyl-(1->4)-6-azido-6-deoxy-D-glucopyranose
-
at pH 4.5 and 30°C
0.74
Galactobiose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C
634
galactose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C
1.53
mannobiose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C
5051
mannose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C
5051
xylose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C
0.022
1,4-benzoquinone
Thermothelomyces myriococcoides
recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30°C
0.056
1,4-benzoquinone
Thermothelomyces myriococcoides
mutant enzyme 700S, in 100 mM McIlvaine buffer, pH 6.0, at 30°C
0.012
2,6-dichloroindophenol
cellobiose as substrate, pH 4.5
44
2,6-dichloroindophenol
Thermothelomyces myriococcoides
pH 7.4, 37°C, mutant enzyme M309K/M409S
84
2,6-dichloroindophenol
Thermothelomyces myriococcoides
pH 7.4, 37°C, mutant enzyme M409S
138
2,6-dichloroindophenol
Thermothelomyces myriococcoides
pH 7.4, 37°C, wild-type enzyme
140.4
2,6-dichloroindophenol
Thermothelomyces myriococcoides
-
lactose as electron donor, at 40°C
196
2,6-dichloroindophenol
Thermothelomyces myriococcoides
pH 7.4, 37°C, mutant enzyme M309V
200
2,6-dichloroindophenol
Thermothelomyces myriococcoides
pH 7.4, 37°C, mutant enzyme M309K
205
2,6-dichloroindophenol
Thermothelomyces myriococcoides
pH 7.4, 37°C, mutant enzyme L324C
219
2,6-dichloroindophenol
Thermothelomyces myriococcoides
pH 7.4, 37°C, mutant enzyme L324C/M409S
350
2,6-dichloroindophenol
Thermothelomyces myriococcoides
pH 7.4, 37°C, mutant enzyme M309K/L324C
0.0049
2,6-dichlorophenol indophenol
-
pH 4.5, 30°C, reaction with lactose
0.0064
2,6-dichlorophenol indophenol
pH 4.5, reaction with cellobiose, wild-type enzyme
0.0074
2,6-dichlorophenol indophenol
pH 4.5, reaction with cellobiose, mutant enzyme M65H
0.01147
2,6-dichlorophenol indophenol
Thermothelomyces myriococcoides
recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30°C
0.033
2,6-dichlorophenol indophenol
Thermothelomyces myriococcoides
mutant enzyme N700S, in 100 mM McIlvaine buffer, pH 6.0, at 30°C
0.078
2,6-dichlorophenol-indophenol
-
in 50 mM MES buffer (pH 6.0), at 25°C
0.018
3,5-di-tert-butyl-1,2-benzoquinone
-
pH 4.5, 30°C, reaction with lactose
0.041
3,5-di-tert-butyl-1,2-benzoquinone
-
pH 4.5, 30°C, reaction with lactose
0.0004
cellobiose
-
electron acceptor 2,2'-azino-di-(3-ethyl-benzthiazolin-6-sulfonic acid) cation radical
0.018
cellobiose
pH 4.5, electron acceptor: cytochrome c, wild-type enzyme
0.021
cellobiose
-
pH 4.5, 23°C, mutant enzyme H689Q or mutant enzyme H689A
0.035
cellobiose
pH 4.5, electron acceptor: 2,6-dichlorophenol indophenol, mutant enzyme M65H
0.035
cellobiose
Thermothelomyces myriococcoides
-
in 100 mM sodium acetate buffer, pH 4.0, at 30°C
0.04
cellobiose
pH 4.5, electron acceptor: 2,6-dichlorophenol indophenol, wild-type enzyme
0.0518
cellobiose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C
0.06
cellobiose
purified wild type enzyme, pH and temperature not specified in the publication
0.075
cellobiose
Thermothelomyces myriococcoides
-
2,6-dichloroindophenol as electron acceptor, at 40°C
0.08
cellobiose
pH 4.5, 30°C, acceptor: 2,6-dichlorophenolindophenol
0.081
cellobiose
Thermothelomyces fergusii
-
recombinant enzyme from Pichia pastoris, with ferricytochrome c as cosubstrate, at pH 7.5 and 30°C
0.102
cellobiose
Thermothelomyces fergusii
-
recombinant enzyme from Aspergillus niger, with ferricytochrome c as cosubstrate, at pH 7.5 and 30°C
0.118
cellobiose
Thermothelomyces fergusii
-
recombinant enzyme from Trichoderma reesei, with ferricytochrome c as cosubstrate, at pH 7.5 and 30°C
0.175
cellobiose
with ferricytochrome c as cosubstrate, at pH 4.5 and 30°C
0.21
cellobiose
-
pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol
0.21
cellobiose
-
pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol
0.285
cellobiose
with 2,6-dichlorophenolindophenol as cosubstrate, at pH 4.5 and 30°C
0.349
cellobiose
Thermothelomyces fergusii
-
recombinant enzyme from Trichoderma reesei, with 2,6-dichlorophenolindophenol as cosubstrate, at pH 5.5 and 30°C
0.357
cellobiose
Thermothelomyces fergusii
-
recombinant enzyme from Aspergillus niger, with 2,6-dichlorophenolindophenol as cosubstrate, at pH 5.5 and 30°C
0.367
cellobiose
Thermothelomyces fergusii
-
recombinant enzyme from Escherichia coli, with 2,6-dichlorophenolindophenol as cosubstrate, at pH 5.5 and 30°C
0.373
cellobiose
Thermothelomyces fergusii
-
recombinant enzyme from Pichia pastoris, with 2,6-dichlorophenolindophenol as cosubstrate, at pH 5.5 and 30°C
0.1224
cellohexaose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C
0.1131
cellopentaose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C
0.46
cellopentaose
-
pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol
0.51
cellopentaose
-
pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol
0.085
cellotetraose
Thermothelomyces myriococcoides
-
in 100 mM sodium acetate buffer, pH 4.0, at 30°C
0.0902
cellotetraose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C
0.42
cellotetraose
-
pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol
0.47
cellotetraose
-
pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol
0.0814
cellotriose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C
0.0008
cytochrome c
pH 4.5, reaction with cellobiose, wild-type enzyme
0.0179
cytochrome c
mutant F166Y, cellobiose oxidation with electron acceptors
0.0286
cytochrome c
wild type, cellobiose oxidation with electron acceptors
2 - 3
cytochrome c
Thermothelomyces myriococcoides
pH 7.4, 37°C, mutant enzyme M309K/L324C
9
cytochrome c
Thermothelomyces myriococcoides
pH 7.4, 37°C, mutant enzyme M309K/M409S
11
cytochrome c
Thermothelomyces myriococcoides
pH 7.4, 37°C, mutant enzyme L324C/M409S
387
D-glucose
Thermothelomyces myriococcoides
-
in 100 mM sodium acetate buffer, pH 4.0, at 30°C
890
D-glucose
-
pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol
1300
D-glucose
-
pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol
2109
D-glucose
pH 4.5, 30°C, acceptor: 2,6-dichlorophenolindophenol
0.0005
ferricytochrome c
Thermothelomyces myriococcoides
-
in the presence of 30 mM NaCl, at pH 7.5 and 30°C
0.0028
ferricytochrome c
Thermothelomyces myriococcoides
-
in the presence of 30 mM NaCl, at pH 5.5 and 30°C
0.0043
ferricytochrome c
Thermothelomyces myriococcoides
-
in the presence of 30 mM SrCl2, at pH 5.5 and 30°C
0.0055
ferricytochrome c
Thermothelomyces myriococcoides
-
in the presence of 30 mM BaCl2, at pH 5.5 and 30°C
0.0056
ferricytochrome c
Thermothelomyces myriococcoides
-
in the presence of 30 mM CaCl2, at pH 5.5 and 30°C
0.0056
ferricytochrome c
Thermothelomyces myriococcoides
-
in the presence of 30 mM MgCl2, at pH 5.5 and 30°C
0.0058
ferricytochrome c
Thermothelomyces myriococcoides
-
in the presence of 30 mM SrCl2, at pH 7.5 and 30°C
0.006
ferricytochrome c
Thermothelomyces myriococcoides
-
in the presence of 30 mM CaCl2, at pH 7.5 and 30°C
0.0061
ferricytochrome c
Thermothelomyces myriococcoides
-
in the presence of 30 mM MgCl2, at pH 7.5 and 30°C
0.0065
ferricytochrome c
Thermothelomyces myriococcoides
-
in the presence of 30 mM BaCl2, at pH 5.5 and 30°C
157.1
glucose
Thermothelomyces myriococcoides
-
2,6-diclorindophenol as electron acceptor, at 40°C
726
glucose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C
0.0172
lactose
Thermothelomyces myriococcoides
-
CDH modified plain screen printed electrodes at 0 mV, pH and temperature not specified in the publication
0.09
lactose
Thermothelomyces myriococcoides
-
in 100 mM sodium acetate buffer, pH 4.0, at 30°C
0.14
lactose
Thermothelomyces myriococcoides
-
2,6-diclorindophenol as electron acceptor, at 40°C
0.2
lactose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C
1.16
lactose
purified wild type enzyme, pH and temperature not specified in the publication
1.42
lactose
pH 4.5, 30°C, acceptor: 2,6-dichlorophenolindophenol
1.85
lactose
with ferricytochrome c as cosubstrate, at pH 4.5 and 30°C
2.4
lactose
-
pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol
3.7
lactose
-
pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol
5.241
lactose
with 2,6-dichlorophenolindophenol as cosubstrate, at pH 4.5 and 30°C
6.75
maltose
Thermothelomyces myriococcoides
-
in 100 mM sodium acetate buffer, pH 4.0, at 30°C
8.86
maltose
Thermothelomyces myriococcoides
-
2,6-diclorindophenol as electron acceptor, at 40°C
350
maltose
-
pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol
390
maltose
-
pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol
5051
maltose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C
0.71
maltotetraose
Thermothelomyces myriococcoides
-
in 100 mM sodium acetate buffer, pH 4.0, at 30°C
0.037
O2
Thermothelomyces myriococcoides
recombinant wild type enzyme, in 100 mM McIlvaine buffer, pH 6.0, at 30°C
0.073
O2
Thermothelomyces myriococcoides
mutant enzyme N700S, in 100 mM McIlvaine buffer, pH 6.0, at 30°C
1.8
thiocellobiose
-
pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol
2.5
thiocellobiose
-
pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol
0.056
ubiquinone
mutant F166Y, cellobiose oxidation with electron acceptors
0.0579
ubiquinone
wild type, cellobiose oxidation with electron acceptors
2.81
xylobiose
-
recombinant enzyme, in 100 mM sodium acetate (pH 4.5), at 30°C
7.3
xylobiose
-
pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol
7.7
xylobiose
-
pH 4.5, 30°C, electron acceptor: 2,6-dichlorophenol indophenol
additional information
cellobiose
addition of polysaccharides modifies the Km-value, depending on incubation temperature and incubation time
additional information
cellobiose
-
addition of polysaccharides modifies the Km-value, depending on incubation temperature and incubation time
additional information
lactose
addition of polysaccharides modifies the Km-value, depending on incubation temperature and incubation time
additional information
lactose
-
addition of polysaccharides modifies the Km-value, depending on incubation temperature and incubation time
