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1.1.99.18: cellobiose dehydrogenase (acceptor)

This is an abbreviated version!
For detailed information about cellobiose dehydrogenase (acceptor), go to the full flat file.

Word Map on EC 1.1.99.18

Reaction

cellobiose
+
acceptor
=
cellobiono-1,5-lactone
 +
reduced acceptor

Synonyms

cbdA, CBO, CBOR, Cdh, CDH IIA, CDH IIB, cdh-1, CDH1, Cdh2, CDHIIA, cellobiose (acceptor) 1-oxidoreductase, cellobiose dehydrogenase, cellobiose dehydrogenase IIA, cellobiose oxidase, cellobiose oxidoreductase, cellobiose [acceptor] 1-oxidoreductase, Cellobiose-quinone oxidoreductase, cellobiose:(acceptor) 1-oxidoreductase, cellobiose:quinone oxidoreductase, cellobiose:[acceptor] 1-oxidoreductase, DCHsr, dehydrogenase, cellobiose, EC 1.1.3.25, EC 1.1.5.1, MtCDH, oxidase, cellobiose, Thite_59724, TpCDH, TvCDH

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.99 With unknown physiological acceptors
                1.1.99.18 cellobiose dehydrogenase (acceptor)

Purification

Purification on EC 1.1.99.18 - cellobiose dehydrogenase (acceptor)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
138fold purification
-
172fold purification, combination of ultrafiltration, ion-exchange and gel-filtration chromatography
-
3-4fold purification
-
70fold purification
-
ammoinium sulfate precipitation, DEAE-Sepharose column chromatography, Sephadex G-50 gel filtration, and lactose-CPG column chromatography
ammonium sulfate precipitation and phenyl Sepharose column chromatography
Thermothelomyces myriococcoides
-
ammonium sulfate precipitation and Resource Q column chromatography
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, phenyl-Sepharose column chromatography, and Sephadex G-50 gel filtration
ammonium sulfate precipitation, ion-exchange chromatography, and chromatofocusing
-
ammonium sulfate precipitation, phenyl Sepharose column chromatography, and Sephacryl S-200 gel filtration
-
ammonium sulfate precipitation, phenyl-Sepharose column chromatography, and Superdex 200 gel filtration
-
ammonium sulfate precipitation, Q Sepharose column chromatography, and hydrophobic interaction chromatography
-
ammonium sulfate, DEAE-Sephadex A50, Sephadex G-100
-
ammonium sulfate, DEAE-Sephadex, Phenyl-Sepharose, Sephacryl S-200, Mono Q
-
ammonium sulfate, Phenyl-Sepharose, Sephacryl S-200, Mono Q
-
arginine sepharose, S-sepharose, HPQ-sepharose, Superdex
-
by hi-trap column and phenyl HIC column chromatography, deglycosylation
Thermothelomyces myriococcoides
-
chelating Sepharose column chromatography
DEAE sepharose, Resource PHE
-
DEAE-Sepharose column chromatography and phenyl-Source column chromatography
diafiltration, ion exchange chromatography, hydrophobic interaction chromatography, ion exchange chromatography
enrichment, virtually free of cellulase
-
enzyme from Phanerochaete chrysosporium and enzyme recombinantly expressed in Trichoderma reesei
His-Bind resin Ni-charged column chromatography
IMAC, phenyl Sepharose column chromatography, and QSource column chromatography,
Thermothelomyces fergusii
-
Mono Q column chromatography and Source 15PHE column chromatography
-
Ni-NTA column chromatography
-
PHE-Sepharose column chromatography and Source 15Q column chromatography
phenyl Sepharose column chromatography and Q-Source column chromatography
Thermothelomyces myriococcoides
ProBond resin (Ni2+) column chromatography
-
purification of CBO and FAD fragment of CBO
-
Q Sepharose, DEAE TSK 650 S, Superose 12
Coniophora puteana (Schum ex Fr) Karsten
-
ultrafiltration, gel filtration, hydrophobic interaction chromatography, gel filtration
-
using A POROS HP 2, gel filtation, a HI Load Superdex-200 and a Mono Q column
-
using a Q-Sepharose anion exchange column, a Phenyl Sepharose hydrophobic interaction column, and gel filtration
-