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1.1.99.18: cellobiose dehydrogenase (acceptor)

This is an abbreviated version!
For detailed information about cellobiose dehydrogenase (acceptor), go to the full flat file.

Word Map on EC 1.1.99.18

Reaction

cellobiose
+
acceptor
=
cellobiono-1,5-lactone
 +
reduced acceptor

Synonyms

cbdA, CBO, CBOR, Cdh, CDH IIA, CDH IIB, cdh-1, CDH1, Cdh2, CDHIIA, cellobiose (acceptor) 1-oxidoreductase, cellobiose dehydrogenase, cellobiose dehydrogenase IIA, cellobiose oxidase, cellobiose oxidoreductase, cellobiose [acceptor] 1-oxidoreductase, Cellobiose-quinone oxidoreductase, cellobiose:(acceptor) 1-oxidoreductase, cellobiose:quinone oxidoreductase, cellobiose:[acceptor] 1-oxidoreductase, DCHsr, dehydrogenase, cellobiose, EC 1.1.3.25, EC 1.1.5.1, MtCDH, oxidase, cellobiose, Thite_59724, TpCDH, TvCDH

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.99 With unknown physiological acceptors
                1.1.99.18 cellobiose dehydrogenase (acceptor)

pH Stability

pH Stability on EC 1.1.99.18 - cellobiose dehydrogenase (acceptor)

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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2 - 11
-
pH 2: below large loss of activity, pH 11: above large loss of activity
389751
2 - 8
-
at least for 12 h, assayed with 2,6-dichlorophenol indophenol
701028
2 - 9
the enzyme is stable for 12 h at pH 3.0-6.0, losing 50% of its initial activity within approximately 3 h at pH 2.0 and 8.0. The enzyme is especially sensitive to pH values above 9.0
739884
3
-
3 h, about 20% loss of activity
763637
3 - 10
-
-
741755
3 - 10.5
-
complete inactivation at pH 2 within 2 h
287779
3 - 8
-
enzyme activities decrease sharply when stored in buffers with pHs below 3.0 or above 8.0
725138
4 - 11
-
at 30°C stable for 24 h, at pH 3.5 almost 40% activity are lost within 12 h
287782
4.5 - 5
-
most stable at
287774, 287775
5 - 6
-
3 h, less than 5% loss of activity
763637
5.7
-
at 35°C, enzyme loses 50% of its initial activity after 10 min at pH 2.2, stability optimum shifts to 5.0 at 46°C with a half-life of 12 h
287787
6
-
55°C, 50% loss of activity after 99 min
654237
6 - 10
the enzyme retains more than 90% of activity at pH below 6 and more than 50% activity at pH 10
763210
8
-
2 h, about 40% loss of activity
763637
9
-
2 h, about 60% loss of activity
763637