1.1.99.20: alkan-1-ol dehydrogenase (acceptor)

This is an abbreviated version!
For detailed information about alkan-1-ol dehydrogenase (acceptor), go to the full flat file.

Word Map on EC 1.1.99.20

Reaction

primary alcohol
+
acceptor
=
aldehyde
 +
reduced acceptor

Synonyms

alkan-1-ol dehydrogenase, alkan-1-ol:acceptor oxidoreductase, dehydrogenase, polyethylene glycol, ethoxy chain nonylphenol dehydrogenase, NPEO-DH, PEG-DH, PEGDH, polyethylene glycol dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.99 With unknown physiological acceptors
                1.1.99.20 alkan-1-ol dehydrogenase (acceptor)

Purification

Purification on EC 1.1.99.20 - alkan-1-ol dehydrogenase (acceptor)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
from a symbiotic mixed culture E-1, consisting of Sphingomonas terrae and a Rhizobium sp.
-
mixed culture, using precipitation with ammonium sulfate, solubilization with laurylbetaine and chromatography with diethylamino-ethyl-cellulose, hydroxylapatite and Sephadex G-200
Ni-NTA affinity chromatography
strain No. 203, by precipitation with ammonium sulfate, solubilization with benzalkonium chloride, chromatography with DEAE-Toyopearl 650M and hydroxylapatite and gel filtration on Toyopearl HW-55
-