1.1.99.28: glucose-fructose oxidoreductase

This is an abbreviated version!
For detailed information about glucose-fructose oxidoreductase, go to the full flat file.

Reaction

Synonyms

EC 1.1.1.99, GFOD2, GFOR, glucose fructose oxidoreductase, glucose-fructose oxidoreductase, glucose-fructose oxidoreductase domain containing 2, Glucose-fructose transhydrogenase, NADP(H)-dependent glucose-fructose oxidoreductase, Transhydrogenase, glucose-fructose

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.99 With unknown physiological acceptors

                1.1.99.28 glucose-fructose oxidoreductase

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
2	Activating Compound
1851	AA Sequence
4	Application
1	CAS Registry Number
6	Cloned(Commentary)
37	Cofactor
4	Crystallization (Commentary)
2	Disease/ Diagnostics
2	General Information
12	General Stability
5	Inhibitors
5	KM Value [mM]
17	Localization
7	Molecular Weight [Da]
23	Natural Substrates/ Products (Substrates)
63	Organism
1	Pathway
30	PDB ID
6	pH Optimum
3	pH Range
2	pH Stability
2	Posttranslational Modification
10	Protein Variants
7	Purification (Commentary)
3	Reaction
2	Reaction Type
42	Reference
2	Source Tissue
7	Specific Activity [micromol/min/mg]
57	Substrates and Products (Substrate)
9	Subunits
35	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
2	Temperature Range [°C]
5	Temperature Stability [°C]
5	Turnover Number [1/s]

Engineering

print visible entries

print all entries

Engineering on EC 1.1.99.28 - glucose-fructose oxidoreductase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide show all columns Go to Protein Variants Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

DELTA1-22/S64D

Zymomonas mobilis

Q07982

S64D mutation converts the strict NADP+ spoecificity of wild-type GFOR to a dual NADP+/NAD+ specificity

656483

DELTA32-46

Zymomonas mobilis

-

the mutant enzyme DELTA32-46 is a protein that is no longer processed but shows full enzymatic activity and has the cofactor firmly bound. The mutant enzyme DELTA2-20 or a mutant enzyme with an exchange of the entire signal sequence with the signal sequence of gluconolactonase of Zymomonas mobilis leads to an active and processed protein

10907

K121A

Zymomonas mobilis

-

mutant enzyme is not processed within 60 min

655405

K123A

Zymomonas mobilis

-

mutant enzyme shows processing behavior comparable to wild-type enzyme

655405

S116D

3 entries

S116D/K121A/K123Q/I124K

Zymomonas mobilis

-

significantly retarded processing kinetics with residual unprocessed form being detectable even after 60 min

655405

Y269F

Zymomonas mobilis

Q07982

essential acid-base catalyst, involved in substrate binding, activity completely abolished

697992

additional information

Zymomonas mobilis

-

mutant enzymes with deletions in the signal peptide are enzymatically active and contain tightly bound NADP(H). Mutant enzymes with a complete deletion of the signal peptide are produced as cytosolic enzymes

10910

S116D

Zymomonas mobilis

Q07982

a single site mutation S116D alters the enzyme which in the wild type situation contains NAD(P)+ as nondissociable redox cofactor reacting in a ping-pong type mechanism to a dehydrogenase with dissociable NAD(P)+ as cosubstrate and a sequential reaction type

10905

S116D

Zymomonas mobilis

-

significantly retarded processing kinetics with residual unprocessed form being detectable even after 60 min

655405

S116D

Zymomonas mobilis

Q07982

dual NADP+/NAD+ specificity with loss of tight cofactor binding, wild type: strict NADP+ specificity

697992