1.1.99.31: (S)-mandelate dehydrogenase

This is an abbreviated version!
For detailed information about (S)-mandelate dehydrogenase, go to the full flat file.

Word Map on EC 1.1.99.31

Reaction

(S)-Mandelate
+
acceptor
=
phenylglyoxylate
+
reduced acceptor

Synonyms

(S)-mandelate dehydrogenase, L-mandelate dehydrogenase, L-MDH, MDH, SManDH, SMDH

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.99 With unknown physiological acceptors
                1.1.99.31 (S)-mandelate dehydrogenase

KM Value

KM Value on EC 1.1.99.31 - (S)-mandelate dehydrogenase

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
22
(R,S)-2-hydroxy-3-butynoic acid
in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C
32
(R,S)-2-hydroxybutyrate
in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C
4.9
(R,S)-2-hydroxyhexanoate
in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C
4.3
(R,S)-2-hydroxyisocaproate
in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C
9.5
(R,S)-2-hydroxyisovalerate
in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C
0.75
(R,S)-2-hydroxyoctanoate
in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C
15.3
(R,S)-2-hydroxyvalerate
in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C
0.9
(R,S)-3-indolelactate
in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C
0.4
(R,S)-indoleglycolate
in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C
0.33
(R,S)-mandelate
in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C
0.074 - 4.3
(S)-3-phenyllactate
0.04 - 73
(S)-Mandelate
0.78 - 2.6
(S)-phenyllactate
10.3
2-Hydroxy-3-butenoate
-
pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach
4.3 - 22
2-Hydroxy-3-butynoate
4.4 - 32
2-Hydroxybutyrate
0.89 - 4.9
2-hydroxyhexanoate
0.49 - 4.3
2-hydroxyisocaproate
2.6
2-Hydroxyisovalerate
-
pH 7.5, 20°C, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach
0.14 - 0.8
2-Hydroxyoctanoate
3.2 - 15.3
2-hydroxyvalerate
0.08
3-hydroxy-D,L-mandelate
-
pH 7.5, 25°C, cosubstrate: ferricyanide
0.13 - 0.9
3-indolelactate
0.12
3-methoxy-D,L-mandelate
-
pH 7.5, 25°C, cosubstrate: ferricyanide
0.37 - 2
3-Phenyllactate
0.26
4-bromo-D,L-mandelate
-
pH 7.5, 25°C, cosubstrate: ferricyanide
0.15 - 0.38
4-chloro-D,L-mandelate
0.27 - 0.43
4-chloromandelate
0.16
4-fluoro-D,L-mandelate
-
pH 7.5, 25°C, cosubstrate: ferricyanide
0.12
4-methoxy-D,L-mandelate
-
pH 7.5, 25°C, cosubstrate: ferricyanide
0.17
4-methyl-D,L-mandelate
-
pH 7.5, 25°C, cosubstrate: ferricyanide
0.35
D,L-Mandelate
-
pH 7.5, 25°C, cosubstrate: ferricyanide
2
DL-3-phenyllactate
in 0.1 M potassium phosphate buffer, pH 7.5 at 20°C
0.47 - 0.78
DL-[2-1H]-mandelate
0.53 - 1.33
DL-[2-2H]-mandelate
2.7 - 3.7
ethyl-(S)-mandelate
3.87
ferricyanide
-
at pH 6.5 and 30°C
0.24 - 0.63
indoleglycolate
0.24
L-Mandelate
-
pH 7.5, 25°C, cosubstrate: ferricyanide
0.04 - 0.24
Mandelate
7.4 - 11.3
methyl-(S)-mandelate
0.04 - 3.2
O2