1.1.99.31: (S)-mandelate dehydrogenase

This is an abbreviated version!
For detailed information about (S)-mandelate dehydrogenase, go to the full flat file.

Word Map on EC 1.1.99.31

Reaction

(S)-Mandelate
+
acceptor
=
phenylglyoxylate
+
reduced acceptor

Synonyms

(S)-mandelate dehydrogenase, L-mandelate dehydrogenase, L-MDH, MDH, SManDH, SMDH

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.99 With unknown physiological acceptors
                1.1.99.31 (S)-mandelate dehydrogenase

Storage Stability

Storage Stability on EC 1.1.99.31 - (S)-mandelate dehydrogenase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70°C, wild-type enzyme can be stored frozen in 20% ethanediol for weeks without loss of activity
-
-70°C, wild-type enzyme retains activity for more than 1 year. The single Arg165 mutant lose activity after 2-3 months, double mutants R165K/R277K and R165G/R277K are inactivated after 2-3 weeks in storage
-