1.1.99.35: soluble quinoprotein glucose dehydrogenase

This is an abbreviated version, for detailed information about soluble quinoprotein glucose dehydrogenase, go to the full flat file.

Reaction

D-glucose
+
acceptor
=
D-glucono-1,5-lactone
+
reduced acceptor

Synonyms

2,7,9-tricarboxypyrroloquinoline quinone-dependent glucose dehydrogenase, GCD, GDH, mGDH, PQQ glucose dehydrogenase, PQQ-GDH, PQQ-glucose dehydrogenase, PQQ-sGDH, PQQGDH, PQQGDH-B, pyrroloquinoline quinone soluble glucose dehydrogenase, pyrroloquinoline quinone-dependent glucose dehydrogenase, pyrroloquinolinequinone-dependent glucose dehydrogenase, s-GDH, sGDH, sGDH-PQQ, soluble PQQ-dependent glucose dehydrogenase, soluble PQQ-GDH, soluble PQQ-glucose dehydrogenase, soluble pyrroloquinoline quinone-dependent glucose dehydrogenase

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.99 With unknown physiological acceptors
                1.1.99.35 soluble quinoprotein glucose dehydrogenase

KM Value

KM Value on EC 1.1.99.35 - soluble quinoprotein glucose dehydrogenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
88 - 90
2-deoxy-D-glucose
2 - 46
3-O-methyl-D-glucose
21 - 38.7
D-Allose
2.7 - 9
D-galactose
2 - 64
D-glucose
14.3 - 34
D-lactose
14.3 - 46.5
D-maltose
22
D-mannose
-
mutant E277K, pH 7.0; wild-type, pH 7.0
7.5 - 14.3
D-xylose
17.5 - 20
lactose
13 - 14
maltose
additional information
additional information
-
under conditions that avoid its masking by sugar-substrate inhibition as much as possible, negative kinetic cooperativity with respect to sugar substrate oxidation is observed. The cooperativity effect dramatically changes the performance of soluble GDH, as reflected by the V2 and K2 values for glucose in phosphate buffer being about 10-fold and 100-fold higher than the V1 and K1 values, respectively. Substituting the Ca2+ involved in activation of pyrroloquinoline quinone in soluble GDH by Sr2+ affects the cooperativity effect but not the two turnover rates of the hybrid enzyme for glucose
-