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0.3
4-O-methyl-alpha-D-glucopyranosyl uronic acid-(1->2)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
wild type enzyme, at pH 8.0 and 37°C
52 - 75
6-deoxy-D-glucose
0.078 - 0.09
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
0.052 - 0.059
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose
0.06 - 0.26
cellotetraose
56 - 950
N-acetyl-D-glucosamine
0.067 - 0.11
xylotetraose
52
6-deoxy-D-glucose
-
O2 used as electron acceptor
75
6-deoxy-D-glucose
-
2,6-dichlorophenol indophenol used as electron acceptor
0.078
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
wild type enzyme, at pH 8.0 and 37°C
0.09
alpha-L-arabinofuranosyl-(1->2)-[alpha-L-arabinofuranosyl-(1->3)]-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-D-xylose
mutant enzyme A38V, at pH 8.0 and 37°C
0.052
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose
mutant enzyme A38V, at pH 8.0 and 37°C
0.059
alpha-L-arabinofuranosyl-(1->3)-beta-D-xylopyranosyl-(1->4)-D-xylose
wild type enzyme, at pH 8.0 and 37°C
0.039
cellobiose
mutant enzyme A38V, at pH 8.0 and 37°C
0.04
cellobiose
wild type enzyme, at pH 8.0 and 37°C
0.05
cellobiose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
0.061
cellobiose
wild type protein
0.07
cellobiose
wild type enzyme, at 37°C and pH 8.0
0.08
cellobiose
-
2,6-dichlorophenol indophenol used as electron acceptor
0.083
cellobiose
mutant enzyme W351F, at 37°C and pH 8.0
0.13
cellobiose
-
O2 used as electron acceptor
0.22
cellobiose
H70A mutant protein
0.25
cellobiose
mutant enzyme Y300A, at 37°C and pH 8.0
0.25
cellobiose
mutant enzyme Y300A, at pH 8.0 and 37°C
0.25
cellobiose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
0.25
cellobiose
pH 8.0, 37°C, recombinant mutant Y300A
0.38
cellobiose
mutant enzyme Y300N, at 37°C and pH 8.0
0.4
cellobiose
C130A mutant protein
0.06
cellohexaose
-
2,6-dichlorophenol indophenol used as electron acceptor
0.08
cellohexaose
wild type enzyme, at pH 8.0 and 37°C
0.08
cellohexaose
mutant enzyme A38V, at pH 8.0 and 37°C
0.1
cellohexaose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
0.16
cellohexaose
-
O2 used as electron acceptor
0.31
cellohexaose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
0.03
cellopentaose
-
2,6-dichlorophenol indophenol used as electron acceptor
0.057
cellopentaose
mutant enzyme A38V, at pH 8.0 and 37°C
0.06
cellopentaose
wild type enzyme, at pH 8.0 and 37°C
0.09
cellopentaose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
0.15
cellopentaose
-
O2 used as electron acceptor
0.3
cellopentaose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
0.06
cellotetraose
-
2,6-dichlorophenol indophenol used as electron acceptor
0.06
cellotetraose
mutant enzyme A38V, at pH 8.0 and 37°C
0.07
cellotetraose
wild type enzyme, at pH 8.0 and 37°C
0.09
cellotetraose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
0.26
cellotetraose
-
O2 used as electron acceptor
0.05
cellotriose
wild type enzyme, at pH 8.0 and 37°C
0.05
cellotriose
mutant enzyme A38V, at pH 8.0 and 37°C
0.06
cellotriose
-
2,6-dichlorophenol indophenol used as electron acceptor
0.08
cellotriose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
0.085
cellotriose
wild type enzyme, at 37°C and pH 8.0
0.11
cellotriose
-
O2 used as electron acceptor
0.11
cellotriose
mutant enzyme W351F, at 37°C and pH 8.0
0.21
cellotriose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
0.25
cellotriose
mutant enzyme Y300A, at 37°C and pH 8.0
0.25
cellotriose
mutant enzyme Y300A, at pH 8.0 and 37°C
0.25
cellotriose
pH 8.0, 37°C, recombinant mutant Y300A
0.44
cellotriose
mutant enzyme Y300N, at 37°C and pH 8.0
36.1
D-galactose
mutant enzyme W351F, at 37°C and pH 8.0
61
D-galactose
-
2,6-dichlorophenol indophenol used as electron acceptor
91
D-galactose
-
O2 used as electron acceptor
96
D-galactose
mutant enzyme Y300A, at 37°C and pH 8.0
110
D-galactose
mutant enzyme Y300N, at 37°C and pH 8.0
132
D-galactose
wild type enzyme, at 37°C and pH 8.0
3.11
D-glucose
mutant enzyme Y300N, at 37°C and pH 8.0
5.4
D-glucose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
8.1
D-glucose
mutant enzyme Y300A, at 37°C and pH 8.0
8.1
D-glucose
mutant enzyme Y300A, at pH 8.0 and 37°C
8.1
D-glucose
pH 8.0, 37°C, recombinant mutant Y300A
12
D-glucose
mutant enzyme A38V, at pH 8.0 and 37°C
15
D-glucose
wild type enzyme, at pH 8.0 and 37°C
15
D-glucose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
17.4
D-glucose
wild type enzyme, at 37°C and pH 8.0
20
D-glucose
-
2,6-dichlorophenol indophenol used as electron acceptor
25
D-glucose
-
O2 used as electron acceptor
31
D-glucose
mutant enzyme W351F, at 37°C and pH 8.0
2 - 3
D-xylose
-
O2 used as electron acceptor
6.7
D-xylose
-
2,6-dichlorophenol indophenol used as electron acceptor
32
D-xylose
mutant enzyme Y300N, at 37°C and pH 8.0
37
D-xylose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
51.8
D-xylose
mutant enzyme Y300A, at 37°C and pH 8.0
51.8
D-xylose
mutant enzyme Y300A, at pH 8.0 and 37°C
51.8
D-xylose
pH 8.0, 37°C, recombinant mutant Y300A
105
D-xylose
wild type enzyme, at 37°C and pH 8.0
118
D-xylose
mutant enzyme A38V, at pH 8.0 and 37°C
118
D-xylose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
129
D-xylose
wild type enzyme, at pH 8.0 and 37°C
288
D-xylose
mutant enzyme W351F, at 37°C and pH 8.0
47
L-arabinose
-
O2 used as electron acceptor
85
L-arabinose
-
2,6-dichlorophenol indophenol used as electron acceptor
0.11
lactose
-
O2 used as electron acceptor
0.19
lactose
-
2,6-dichlorophenol indophenol used as electron acceptor
13
maltohexaose
-
O2 used as electron acceptor
30
maltohexaose
-
2,6-dichlorophenol indophenol used as electron acceptor
11
maltopentaose
-
2,6-dichlorophenol indophenol used as electron acceptor
32
maltopentaose
-
O2 used as electron acceptor
2.81
maltose
wild type enzyme, at 37°C and pH 8.0
4.9
maltose
-
2,6-dichlorophenol indophenol used as electron acceptor
5
maltose
mutant enzyme W351F, at 37°C and pH 8.0
11
maltose
-
O2 used as electron acceptor
11
maltose
mutant enzyme Y300A, at 37°C and pH 8.0
19.6
maltose
mutant enzyme Y300N, at 37°C and pH 8.0
12
maltotetraose
-
2,6-dichlorophenol indophenol used as electron acceptor
30
maltotetraose
-
O2 used as electron acceptor
18
maltotriose
-
2,6-dichlorophenol indophenol used as electron acceptor
30
maltotriose
-
O2 used as electron acceptor
56
N-acetyl-D-glucosamine
mutant enzyme Y300N, at 37°C and pH 8.0
92
N-acetyl-D-glucosamine
mutant enzyme Y300A, at 37°C and pH 8.0
340
N-acetyl-D-glucosamine
wild type enzyme, at 37°C and pH 8.0
950
N-acetyl-D-glucosamine
mutant enzyme W351F, at 37°C and pH 8.0
0.05
xylobiose
mutant enzyme A38V, at pH 8.0 and 37°C
0.057
xylobiose
wild type enzyme, at pH 8.0 and 37°C
0.09
xylobiose
-
O2 used as electron acceptor
0.098
xylobiose
wild type enzyme, at 37°C and pH 8.0
0.099
xylobiose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
0.21
xylobiose
-
2,6-dichlorophenol indophenol used as electron acceptor
0.35
xylobiose
mutant enzyme W351F, at 37°C and pH 8.0
4.83
xylobiose
mutant enzyme Y300N, at 37°C and pH 8.0
5.11
xylobiose
mutant enzyme Y300A, at 37°C and pH 8.0
5.11
xylobiose
mutant enzyme Y300A, at pH 8.0 and 37°C
5.11
xylobiose
pH 8.0, 37°C, recombinant mutant Y300A
5.84
xylobiose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
0.005
xylohexaose
mutant enzyme A38V, at pH 8.0 and 37°C
0.06
xylohexaose
wild type enzyme, at pH 8.0 and 37°C
0.1
xylohexaose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
3
xylohexaose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
0.05
xylopentaose
wild type enzyme, at pH 8.0 and 37°C
0.05
xylopentaose
mutant enzyme A38V, at pH 8.0 and 37°C
0.104
xylopentaose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
4.49
xylopentaose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
0.067
xylotetraose
mutant enzyme A38V, at pH 8.0 and 37°C
0.07
xylotetraose
wild type enzyme, at pH 8.0 and 37°C
0.11
xylotetraose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
0.08
xylotriose
wild type enzyme, at pH 8.0 and 37°C
0.08
xylotriose
mutant enzyme A38V, at pH 8.0 and 37°C
0.1
xylotriose
wild type enzyme, at 37°C and pH 8.0
0.1
xylotriose
mutant enzyme A38V/S388N, at pH 8.0 and 37°C
0.31
xylotriose
mutant enzyme W351F, at 37°C and pH 8.0
3.15
xylotriose
mutant enzyme Y300A, at 37°C and pH 8.0
3.15
xylotriose
mutant enzyme Y300A, at pH 8.0 and 37°C
3.15
xylotriose
pH 8.0, 37°C, recombinant mutant Y300A
4.3
xylotriose
mutant enzyme Y300N, at 37°C and pH 8.0
4.57
xylotriose
pH 8.0, 37°C, recombinant mutant CtCBM22A_Y300A
1.1.99.B3 glucooligosaccharide oxidase
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