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1.10.3.2: laccase

This is an abbreviated version!
For detailed information about laccase, go to the full flat file.

Word Map on EC 1.10.3.2

Reaction

4 benzenediol +

O2
= 4 benzosemiquinone + 2 H2O

Synonyms

ATM, benzenediol oxygen oxidoreductase, benzenediol-oxygen oxidoreductase, benzenediol: oxygen oxidoreductase, benzenediol:O2 oxidoreductase, Benzenediol:oxygen oxidoreductase, blue laccase, blue multicopper oxidase, CcLCC6, CotA, CotA laccase, CotA-laccase, CotA-type laccase, CueO, DA2_0547, DcLac1, DcLac2, Diphenol oxidase, EpoA, Ery4, Ery4 laccase, FpLcc1, FpLcc2, GMET_RS10855, Hvo_B0205, LAC, Lac I, Lac II, Lac-3.5, Lac-4.8, LAC1, Lac2, Lac2a, LAC3, Lac4, LacA, Lacc, laccase, laccase 1, laccase 3, laccase A, Laccase allele OR, Laccase allele TS, laccase CueO, laccase POXA3b, laccase-2, laccase2, LacCh, lacTT, LacTv, LacZ1, Lcc, lcc1, Lcc2, Lcc3, Lcc4, Lcc9, LccA, lccdelta, lccgamma, Ligninolytic phenoloxidase, MAL, McoP, MmPPO laccase, MmPPOA, More, MSK laccase, multicopper oxidase, p-benzenediol:dioxygen oxidoreductase, p-diphenol dioxygen oxidoreductase, p-diphenol oxidase, p-diphenol: dioxygen oxidoreductase, p-diphenol:dioxygenoxidoreductase, p-diphenol:O2 oxidoreductase, p-diphenol:oxygen oxidoreductase, p-diphenol:oxygen-oxidoreductase, PCL, phenol oxidase, PM1 laccase, polyphenol oxidase A, POXA1b, POXA1w, POXA2, POXA3 laccase, POXA3a, POXA3b, POXC, PpoA, PsLac1, PsLac2, rlac1338, SLAC, SN4LAC, spore coat A protein, spore coat protein A, SRL1, SvLAC13, SvLAC15, SvLAC50, SvLAC52, SvLAC9, TaLac1, ThL, TTC1370, TthLAC, two-domain laccase, urishiol oxidase, urushiol oxidase, Wlac, YacK, yellow laccase

ECTree

     1 Oxidoreductases
         1.10 Acting on diphenols and related substances as donors
             1.10.3 With oxygen as acceptor
                1.10.3.2 laccase

Activating Compound

Activating Compound on EC 1.10.3.2 - laccase

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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-hydroxybenzotriazole
-
highly activating
10-(3-propylsulfonate) phenoxazine
2-chlorophenol
-
activates, effects on enzyme kinetics with substrate N,N-dimethyl-4-phenylenediamine, overview
2-Fluorophenol
-
activates, effects on enzyme kinetics with substrate N,N-dimethyl-4-phenylenediamine, overview
4-Chlorophenol
-
activates, effects on enzyme kinetics with substrate N,N-dimethyl-4-phenylenediamine, overview
4-Fluorophenol
-
activates, effects on enzyme kinetics with substrate N,N-dimethyl-4-phenylenediamine, overview
acetate
the oxidizing activity of CueO for ABTS is enhanced by the addition of up to 300 mM acetate ion, although it decreases at higher concentrations
acetic acid
FpLcc2 is activated by acetic acid up to 3.7times. This activation effect is much more pronounced at pH 5.0 compared to pH 3.0 and can already be observed at a concentration of 1 mM
Ca2+
-
5 mM, 9-13% activation (depending on purification method)
CuSO4
-
activates 113% at over 40 mM with substrate 3,6-dimethoxyphenol, and 46% at over 10 mM with substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
dithiothreitol
-
activates laccase at lower concentration (0.1 mM) while inhibites the enzyme at concentrations beyond 0.1 mM in a concentration-dependent manner with complete inhibition inactivity at 10 mM
Fe2+
-
substrate hydroquinone
glycerol
-
20% (v/v), 15% increase inactivity
H2O2
-
slight activation, increased by Mn2+
hexane
-
25%, activates to 106.13% of control
MnSO4
-
activates 49% at over 80 mM with substrate 3,6-dimethoxyphenol, and 28% at over 80 mM with substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
oxalic acid
-
activates 42% at 2 mM with substrate 3,6-dimethoxyphenol, and 303% at 20 mM with substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
Triton-X-100
-
activated by addition of 0.1% (v/v) Triton-X-100
Tween-40
-
0.5% (v/v), 10% increase in activity
Urea
MK290990.1
100 mM, 1.06fold activation
additional information
-