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0.011
-
CE, purification of Pseudomonas putida F6 laccase-type activity
0.017
native enzyme in pharate pupa and pupal wing
0.018
Loweporus lividus
-
crude enzyme, in 50 mM sodium malonate buffer, pH 4.5 at 37°C
0.052
wild-type, phosphate buffer, pH 6, 25°C
0.061
-
DEAE, purification of Pseudomonas putida F6 laccase-type activity
0.089
substrate: 1-naphthol, pH and temperature not specified in the publication, mutant enzyme V281A/P309L/S318G/D232V
0.13
substrate: 1-naphthol, pH and temperature not specified in the publication, wild-type enzyme
0.14
wild-type, citrate buffer, pH 5.5, 25°C
0.41
purified native enzyme, substrate syringaldazine
0.42
wild-type, acetate buffer, pH 5.5, 25°C
0.45
substrate 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate), wild-type, pH 5.5, 25°C
0.53
substrate p-phenylendiamine, wild-type, pH 5.5, 25°C
0.573
-
Resource Q, purification of Pseudomonas putida F6 laccase-type activity
0.64
substrate: guaiacol, pH and temperature not specified in the publication, wild-type enzyme
0.85
-
culture filtrate, pH 4.5, at 37°C, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate
1
-
substrate: syringaldazine, pH 8.0, 22°C
1.12
substrate: 2,6-dimethoxyphenol, pH and temperature not specified in the publication, wild-type enzyme
1.34
purified recombinant enzyme, substrate 2,6-dimethoxyphenol, pH 5.0, 25°C
1.37
substrate: guaiacol, pH and temperature not specified in the publication, mutant enzyme V281A/P309L/S318G/D232V
1.52
-
recombinant enzyme from Escherichia coli AH3517 cells grown under aerobic conditions
1.66 - 28.67
-
micromol O2/min/mg depending on organism and isozyme
1.71
-
crude extracellular enzyme in culture
1.95
pH and temperature not specified in the publication
10.06
purified native enzyme, substrate hydrochinone
100
partially purified recombinant truncated mutant POXA1bDELTA4, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
1050
-
25°C, isozyme POXA3b
1070
-
substrate syringaldazine, pH 6.0
112.6
-
pH 4.0, 60°C, purified enzyme, substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
12.8
-
pH 4.0, 60°C, purified enzyme, substrate 1,2,3-trihydroxybenzene
1200
-
substrate quinol, secretion
129
partially purified recombinant truncated mutant POXA1bDELTA16, substrate 2,6-dimethoxyphenol
13
recombinant enzyme, substrate: syringaldazine, pH 6.5, 37°C, recombinant enzyme
13.17
-
pH and temperature not specified in the publication, crude enzyme
13.2
-
substrate: catechol, pH 4.0, 25°C
13.5
-
substrate 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid), pH 3.0
139.4
FJ560721
purified native enzyme, pH 5.0, 22-25°C
144.3
specific activity of the crude laccase broth, pH 3.0, 50°C
148
-
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid), pH 4.0, 25°C
15.8
-
substrate: syringaldazine, pH 4.0, 25°C
150
-
substrate guaiacol, pH 6.0
155
partially purified recombinant wild-type enzyme, substrate 2,6-dimethoxyphenol
16
substrate 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate), wild-type, presence of 1 mM Cu2+, pH 5.5, 25°C
16.39
-
pH and temperature not specified in the publication
16.7
-
substrate: guaiacol, pH 4.0, 25°C
162
partially purified recombinant wild-type enzyme, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
17.3
-
purified native enzyme
17.8
-
purified native enzyme, pH 6.0, 25°C
18.4
-
after 21.6fold purification, pH 4.5, at 37°C, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate
18.54
-
purified native extracellular enzyme, pH 5.0, 50°C
185.4
30°C, pH 4.5, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
189
partially purified recombinant truncated mutant POXA1bDELTA4, substrate 2,6-dimethoxyphenol
19.1
-
crude enzyme, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, at pH 3.0 and 50°C
19.3
-
substrate: guaiacol, pH 7.5, 37°C
2.4
-
partially purified enzyme
2.5
-
purified native enzyme
2.67
substrate: 2,6-dimethoxyphenol, pH and temperature not specified in the publication, mutant enzyme V281A/P309L/S318G/D232V
2.8
-
substrate 2,6-dimethoxyphenol, pH 4.6
20.5
-
purified isozyme laccase III
2000
-
25°C, isozyme POXA3a
22.3
-
purified isozyme laccase II
22.8
substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), pH and temperature not specified in the publication, wild-type enzyme
228.2
-
recombinant enzyme from Escherichia coli AH3517 cells grown under microaerobic conditions
23.5
-
substrate: 2,6-dimethoxyphenol, pH 4.0, 25°C
24 - 36.6
recombinant enzyme expressed in Saccharomyces cerevisiae
24.4
-
purified recombinant wild-type Wlac in presence of 100 mM Cu2+
261
-
purified enzyme, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, at pH 3.0 and 50°C
28.8
pH 4.5, 55°C, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
29
substrate 2,6-dimethoxyphenol, wild-type, presence of 1 mM Cu2+, pH 5.5, 25°C
3
partially purified recombinant truncated mutant POXA1bD205R, substrate 2,6-dimethoxyphenol
3.33
-
purified native extracelluar enzyme, pH 4.0, 40°C
3.4
-
substrate syringaldazine, pH 5.6
30
substrate 2,6-dimethoxyphenol, region Pro357-His406 deletion mutant, presence of 1 mM Cu2+, pH 5.5, 25°C
30 - 35
-
native enzyme in optimized culture
313
-
pH 4.5, temperature not specified in the publication
3138
pH and temperature not specified in the publication, laccase activity in the culture supernatant
34
substrate 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate), region Pro357-His406 deletion mutant, presence of 1 mM Cu2+, pH 5.5, 25°C
358.7
-
after 69.1fold purification, at 30°C, pH 4.5
36.9
-
substrate: hydroquinone, pH 4.0, 25°C
360
-
purified native enzyme
37
Thermochaetoides thermophila
-
pH 6.0, 50°C
38.4 - 50.4
recombinant enzyme expressed in Trichoderma reesei
39.7
-
substrate: ferulic acid, pH 4.0, 25°C
4
substrate 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate), pH 5, 30°C
4.02
purified native enzyme, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
4.18
purified native enzyme, substrate N-beta-alanyldopamine-alanyldopamine
4.57
-
gel filtration, purification of Pseudomonas putida F6 laccase-type activity
4.6
substrate p-phenylendiamine, region Pro357-His406 deletion mutant, pH 5.5, 25°C
400
-
substrate 2,6-dimethoxypenol, pH 6.0
4000
purified recombinant enzyme
417.7
-
purified enzyme, substrate 2,6-dimethoxyphenol, pH 5.0, 50°C
44.5
-
substrate quinol, secretion
460
purified enzyme, substrate 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
498.9
-
pH 8.0, 40°C, substrate: 2,6-dimethoxyphenol
5.01
purified native enzyme, substrate N-acetyldopamine
5.19
-
crude culture, at 30°C, pH 4.5
5.8
-
purified enzyme, substrate syringaldazine
50.1
-
substrate: phloroglucinol, pH 4.0, 25°C
51.4
-
pH 4.0, 60°C, purified enzyme, substrate catechol
52.9
-
purified recombinant mutantWlacS in presence of 100 mM Cu2+
59.27
purified recombinant enzyme, pH 3.0, 30°C
60.8
-
purified native extracellular enzyme, pH 5.0, temperature not specified in the publication, substrate 2,6-dimethoxyphenol
61.2
-
pH 4.0, 60°C, purified enzyme, substrate guaiacol
6322
-
native enzyme in optimized culture
644
-
purified recombinant enzyme truncation mutant, pH 3.2, 37°C
69
purified native enzyme, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
71.6
-
pH 4.0, 60°C, purified enzyme, substrate toluidine
79.8
substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), pH and temperature not specified in the publication, mutant enzyme V281A/P309L/S318G/D232V
8
-
purified recombinant enzyme, with substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid at pH 4.0
83.47
-
pH and temperature not specified in the publication, purified enzyme
85.1
-
purified recombinant mutantWlacD in presence of 100 mM Cu2+
850
-
substrate 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid), pH 4.5
9.1
-
substrate: poyrogallol, pH 4.0, 25°C
952
-
puriifed native extracellular enzyme, pH 3.0, 22°C
978.34
pH 5.0, temperature not specified in the publication
1.41
Loweporus lividus
-
after 67.6fold purification, in 50 mM sodium malonate buffer, pH 4.5 at 37°C
1.41
Loweporus lividus MTCC-1178
-
-
12
substrate catechol, wild-type, presence of 1 mM Cu2+, pH 5.5, 25°C
12
partially purified recombinant truncated mutant POXA1bDELTA16, substrate syringaldazine
15
substrate 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate), region Pro357-His406 deletion mutant, pH 5.5, 25°C
15
partially purified recombinant wild-type enzyme, substrate syringaldazine
17
partially purified recombinant truncated mutant POXA1bDELTA4, substrate syringaldazine
17
-
pH 4.0, 60°C, purified enzyme, substrate 3-aminobenzoic acid
19
substrate p-phenylendiamine, wild-type, presence of 1 mM Cu2+, pH 5.5, 25°C
19
-
purified isozyme laccase I
20
substrate p-phenylendiamine, region Pro357-His406 deletion mutant, presence of 1 mM Cu2+, pH 5.5, 25°C
20
-
purified enzyme, substrate syringaldazine
33
partially purified recombinant truncated mutant POXA1bD205R, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
33
partially purified recombinant truncated mutant POXA1bDELTA16, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
52
-
pH 4.5, 25°C
52
substrate catechol, region Pro357-His406 deletion mutant, presence of 1 mM Cu2+, pH 5.5, 25°C
5778
-
-
5778
-
substrate quinol, secretion
86.5
-
pH 4.0, 60°C, purified enzyme, substrate 2,6-dimethoxyphenol
86.5
-
pH 4.0, 60°C, purified enzyme, substrate guaiacol
additional information
-
depending on isozyme, substrate and pH
additional information
-
-
additional information
-
depending on isozyme, substrate and pH
additional information
-
-
additional information
-
depending on isozyme, substrate and pH
additional information
-
-
additional information
-
the specific activity of the crude enzyme from culture broth with small amount of brown impurities is 451 units/mg and the specific activity of the 11fold purified enzyme is 5111 units/mg. The specific activity of the crude enzyme from culture broth with big amount of brown impurities is 410 units/mg and the specific activity of the 11.38fold purified enzyme is 4666 units/mg. One unit of proteolytic activity is defined as the amount of enzyme that induces absorbance increase of 1.0 per 10 min at 37°C
additional information
-
depending on isozyme, substrate and pH
additional information
Fomes sclerodermeus
-
-
additional information
-
the specific activity of the crude enzyme in 67 mM sodium acetate buffer (pH 4.5), at 30°C, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate is 11.3 units/mg, the specific activity of the 131fold purification enzyme in 67 mM sodium acetate buffer (pH 4.5), at 30°C, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate is 1480 units/mg. One unit of enzyme activity is defined as the amount of enzyme required to produce an absorbance increase at 420 nm of 1 per minute per ml of reaction mixture
additional information
-
-
additional information
-
depending on isozyme, substrate and pH
additional information
-
-
additional information
-
depending on isozyme, substrate and pH
additional information
-
depending on isozyme, substrate and pH
additional information
-
depending on isozyme, substrate and pH
additional information
-
depending on isozyme, substrate and pH
additional information
-
depending on isozyme, substrate and pH
additional information
-
depending on isozyme, substrate and pH
additional information
Thermochaetoides thermophila
-
depending on isozyme, substrate and pH
additional information
-
-
additional information
-
substrate specificity, overview
additional information
-
-
additional information
-
depending on isozyme, substrate and pH
additional information
-
depending on isozyme, substrate and pH
additional information
-
relative activity of laccase in conjugation with chitosans of different molecular weights, overview