Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.10.3.2: laccase

This is an abbreviated version!
For detailed information about laccase, go to the full flat file.

Word Map on EC 1.10.3.2

Reaction

4 benzenediol +

O2
= 4 benzosemiquinone + 2 H2O

Synonyms

ATM, benzenediol oxygen oxidoreductase, benzenediol-oxygen oxidoreductase, benzenediol: oxygen oxidoreductase, benzenediol:O2 oxidoreductase, Benzenediol:oxygen oxidoreductase, blue laccase, blue multicopper oxidase, CcLCC6, CotA, CotA laccase, CotA-laccase, CotA-type laccase, CueO, DA2_0547, DcLac1, DcLac2, Diphenol oxidase, EpoA, Ery4, Ery4 laccase, FpLcc1, FpLcc2, GMET_RS10855, Hvo_B0205, LAC, Lac I, Lac II, Lac-3.5, Lac-4.8, LAC1, Lac2, Lac2a, LAC3, Lac4, LacA, Lacc, laccase, laccase 1, laccase 3, laccase A, Laccase allele OR, Laccase allele TS, laccase CueO, laccase POXA3b, laccase-2, laccase2, LacCh, lacTT, LacTv, LacZ1, Lcc, lcc1, Lcc2, Lcc3, Lcc4, Lcc9, LccA, lccdelta, lccgamma, Ligninolytic phenoloxidase, MAL, McoP, MmPPO laccase, MmPPOA, More, MSK laccase, multicopper oxidase, p-benzenediol:dioxygen oxidoreductase, p-diphenol dioxygen oxidoreductase, p-diphenol oxidase, p-diphenol: dioxygen oxidoreductase, p-diphenol:dioxygenoxidoreductase, p-diphenol:O2 oxidoreductase, p-diphenol:oxygen oxidoreductase, p-diphenol:oxygen-oxidoreductase, PCL, phenol oxidase, PM1 laccase, polyphenol oxidase A, POXA1b, POXA1w, POXA2, POXA3 laccase, POXA3a, POXA3b, POXC, PpoA, PsLac1, PsLac2, rlac1338, SLAC, SN4LAC, spore coat A protein, spore coat protein A, SRL1, SvLAC13, SvLAC15, SvLAC50, SvLAC52, SvLAC9, TaLac1, ThL, TTC1370, TthLAC, two-domain laccase, urishiol oxidase, urushiol oxidase, Wlac, YacK, yellow laccase

ECTree

     1 Oxidoreductases
         1.10 Acting on diphenols and related substances as donors
             1.10.3 With oxygen as acceptor
                1.10.3.2 laccase

Specific Activity

Specific Activity on EC 1.10.3.2 - laccase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.011
-
CE, purification of Pseudomonas putida F6 laccase-type activity
0.017
native enzyme in pharate pupa and pupal wing
0.018
Loweporus lividus
-
crude enzyme, in 50 mM sodium malonate buffer, pH 4.5 at 37°C
0.052
wild-type, phosphate buffer, pH 6, 25°C
0.061
-
DEAE, purification of Pseudomonas putida F6 laccase-type activity
0.089
substrate: 1-naphthol, pH and temperature not specified in the publication, mutant enzyme V281A/P309L/S318G/D232V
0.13
substrate: 1-naphthol, pH and temperature not specified in the publication, wild-type enzyme
0.14
wild-type, citrate buffer, pH 5.5, 25°C
0.402
-
purified enzyme
0.41
purified native enzyme, substrate syringaldazine
0.42
wild-type, acetate buffer, pH 5.5, 25°C
0.45
substrate 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate), wild-type, pH 5.5, 25°C
0.53
substrate p-phenylendiamine, wild-type, pH 5.5, 25°C
0.573
-
Resource Q, purification of Pseudomonas putida F6 laccase-type activity
0.64
substrate: guaiacol, pH and temperature not specified in the publication, wild-type enzyme
0.85
-
culture filtrate, pH 4.5, at 37°C, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate
1
-
substrate: syringaldazine, pH 8.0, 22°C
1.12
substrate: 2,6-dimethoxyphenol, pH and temperature not specified in the publication, wild-type enzyme
1.34
purified recombinant enzyme, substrate 2,6-dimethoxyphenol, pH 5.0, 25°C
1.37
substrate: guaiacol, pH and temperature not specified in the publication, mutant enzyme V281A/P309L/S318G/D232V
1.52
-
recombinant enzyme from Escherichia coli AH3517 cells grown under aerobic conditions
1.66 - 28.67
-
micromol O2/min/mg depending on organism and isozyme
1.71
-
crude extracellular enzyme in culture
1.95
pH and temperature not specified in the publication
10.06
purified native enzyme, substrate hydrochinone
100
partially purified recombinant truncated mutant POXA1bDELTA4, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
1050
-
25°C, isozyme POXA3b
1070
-
substrate syringaldazine, pH 6.0
1077
-
purified enzyme
112.6
-
pH 4.0, 60°C, purified enzyme, substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
12.8
-
pH 4.0, 60°C, purified enzyme, substrate 1,2,3-trihydroxybenzene
1200
-
substrate quinol, secretion
129
partially purified recombinant truncated mutant POXA1bDELTA16, substrate 2,6-dimethoxyphenol
13
recombinant enzyme, substrate: syringaldazine, pH 6.5, 37°C, recombinant enzyme
13.17
-
pH and temperature not specified in the publication, crude enzyme
13.2
-
substrate: catechol, pH 4.0, 25°C
13.5
-
substrate 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid), pH 3.0
130.5
-
purified enzyme
139.4
FJ560721
purified native enzyme, pH 5.0, 22-25°C
14.7
-
25°C, pH 4.5
144.3
specific activity of the crude laccase broth, pH 3.0, 50°C
148
-
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid), pH 4.0, 25°C
15.8
-
substrate: syringaldazine, pH 4.0, 25°C
150
-
substrate guaiacol, pH 6.0
155
partially purified recombinant wild-type enzyme, substrate 2,6-dimethoxyphenol
16
substrate 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate), wild-type, presence of 1 mM Cu2+, pH 5.5, 25°C
16.39
-
pH and temperature not specified in the publication
16.7
-
substrate: guaiacol, pH 4.0, 25°C
1610
-
pH 4.5, 30°C
162
partially purified recombinant wild-type enzyme, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
164
-
pH 6, 25°C
17.3
-
purified native enzyme
17.8
-
purified native enzyme, pH 6.0, 25°C
18.4
-
after 21.6fold purification, pH 4.5, at 37°C, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate
18.54
-
purified native extracellular enzyme, pH 5.0, 50°C
185.4
30°C, pH 4.5, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
189
partially purified recombinant truncated mutant POXA1bDELTA4, substrate 2,6-dimethoxyphenol
19.1
-
crude enzyme, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, at pH 3.0 and 50°C
19.3
-
substrate: guaiacol, pH 7.5, 37°C
19750
-
pH 6.5, 40°C
2.4
-
partially purified enzyme
2.5
-
purified native enzyme
2.67
substrate: 2,6-dimethoxyphenol, pH and temperature not specified in the publication, mutant enzyme V281A/P309L/S318G/D232V
2.8
-
substrate 2,6-dimethoxyphenol, pH 4.6
20.5
-
purified isozyme laccase III
2000
-
25°C, isozyme POXA3a
206
-
purified enzyme
21.6
-
purified enzyme
22.3
-
purified isozyme laccase II
22.8
substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), pH and temperature not specified in the publication, wild-type enzyme
225.9
-
30°C, pH 6.0
226
purified enzyme
228.2
-
recombinant enzyme from Escherichia coli AH3517 cells grown under microaerobic conditions
23.5
-
substrate: 2,6-dimethoxyphenol, pH 4.0, 25°C
234
pH 4.5, 65°C
24 - 36.6
recombinant enzyme expressed in Saccharomyces cerevisiae
24.4
-
purified recombinant wild-type Wlac in presence of 100 mM Cu2+
25
pH 8.4, 45°C
261
-
purified enzyme, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, at pH 3.0 and 50°C
28.8
pH 4.5, 55°C, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)
29
substrate 2,6-dimethoxyphenol, wild-type, presence of 1 mM Cu2+, pH 5.5, 25°C
3
partially purified recombinant truncated mutant POXA1bD205R, substrate 2,6-dimethoxyphenol
3.33
-
purified native extracelluar enzyme, pH 4.0, 40°C
3.4
-
substrate syringaldazine, pH 5.6
30
substrate 2,6-dimethoxyphenol, region Pro357-His406 deletion mutant, presence of 1 mM Cu2+, pH 5.5, 25°C
30 - 35
-
native enzyme in optimized culture
300
-
pH 5.5, 20°C
305
-
pH 3.0, 30°C
313
-
pH 4.5, temperature not specified in the publication
3138
pH and temperature not specified in the publication, laccase activity in the culture supernatant
317
-
purified enzyme
34
substrate 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate), region Pro357-His406 deletion mutant, presence of 1 mM Cu2+, pH 5.5, 25°C
340.8
-
purified enzyme
358.7
-
after 69.1fold purification, at 30°C, pH 4.5
36.9
-
substrate: hydroquinone, pH 4.0, 25°C
360
-
purified native enzyme
37
Thermochaetoides thermophila
-
pH 6.0, 50°C
377.2
-
pH 4.5, 40°C
38.4 - 50.4
recombinant enzyme expressed in Trichoderma reesei
39.7
-
substrate: ferulic acid, pH 4.0, 25°C
4
substrate 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate), pH 5, 30°C
4.02
purified native enzyme, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
4.18
purified native enzyme, substrate N-beta-alanyldopamine-alanyldopamine
4.57
-
gel filtration, purification of Pseudomonas putida F6 laccase-type activity
4.6
substrate p-phenylendiamine, region Pro357-His406 deletion mutant, pH 5.5, 25°C
400
-
substrate 2,6-dimethoxypenol, pH 6.0
4000
purified recombinant enzyme
4022
-
purified enzyme
41.3
-
pH 7.5, 37°C
417.7
-
purified enzyme, substrate 2,6-dimethoxyphenol, pH 5.0, 50°C
44.5
-
substrate quinol, secretion
460
purified enzyme, substrate 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)
498.9
-
pH 8.0, 40°C, substrate: 2,6-dimethoxyphenol
5.01
purified native enzyme, substrate N-acetyldopamine
5.19
-
crude culture, at 30°C, pH 4.5
5.8
-
purified enzyme, substrate syringaldazine
50.1
-
substrate: phloroglucinol, pH 4.0, 25°C
500
-
purified enzyme
51.4
-
pH 4.0, 60°C, purified enzyme, substrate catechol
52.9
-
purified recombinant mutantWlacS in presence of 100 mM Cu2+
576
-
purified enzyme
58.5
-
pH 7.0 37°C
59.27
purified recombinant enzyme, pH 3.0, 30°C
60.8
-
purified native extracellular enzyme, pH 5.0, temperature not specified in the publication, substrate 2,6-dimethoxyphenol
600
-
purified enzyme
61.2
-
pH 4.0, 60°C, purified enzyme, substrate guaiacol
6322
-
native enzyme in optimized culture
644
-
purified recombinant enzyme truncation mutant, pH 3.2, 37°C
66.8
pH 4.5, 25°C
670
pH 4.5, 25°C
69
purified native enzyme, substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid
7.1
purified enzyme
71.6
-
pH 4.0, 60°C, purified enzyme, substrate toluidine
79
pH 4.5, 25°C
79.8
substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), pH and temperature not specified in the publication, mutant enzyme V281A/P309L/S318G/D232V
8
-
purified recombinant enzyme, with substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid at pH 4.0
80
Myrioconium sp.
-
-
83.47
-
pH and temperature not specified in the publication, purified enzyme
85.1
-
purified recombinant mutantWlacD in presence of 100 mM Cu2+
850
-
substrate 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid), pH 4.5
9.1
-
substrate: poyrogallol, pH 4.0, 25°C
91440
-
purified enzyme
952
-
puriifed native extracellular enzyme, pH 3.0, 22°C
978.34
pH 5.0, temperature not specified in the publication
additional information