1.10.3.3: L-ascorbate oxidase

This is an abbreviated version!
For detailed information about L-ascorbate oxidase, go to the full flat file.

Word Map on EC 1.10.3.3

Reaction

4 L-ascorbate +

O2
= 4 monodehydroascorbate + 2 H2O

Synonyms

AA oxidase, AA-ox, AAO, AAO1, AAO2, AAO3, Aao4, AO1, AO4, AOase, ASC oxidase, ascorbase, ascorbate dehydrogenase, ascorbate oxidase, ascorbic acid oxidase, ascorbic oxidase, ASOM, L-ascorbate:O2 oxidoreductase, L-ascorbic acid oxidase, oxidase, ascorbate

ECTree

     1 Oxidoreductases
         1.10 Acting on diphenols and related substances as donors
             1.10.3 With oxygen as acceptor
                1.10.3.3 L-ascorbate oxidase

Cloned

Cloned on EC 1.10.3.3 - L-ascorbate oxidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of cDNA
co-expression of cytochrome b561 and ascorbate oxidase
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expressed in Nicotiana benthamiana and Arabidopsis thaliana
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expressed in Nicotiana benthamiana trichomes, leaves and roots
expressed in Nicotiana tabacum BY-2 cells
expressed in Populus tremula x Populus alba
expression of cDNA in Nicotiana tabacum cell culture
Cucurbita sp.
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expression of RNAi constructs in tomato plants using transfection of WVa106 tomato cotyledons via Agrobacterium strain GV3101, real-time quantitative PCR expression analysis
expression of wild-type, Q183R, T527A, E177K, V185K, V188R, P190I, V193P, R194Q, Q382G, H383Y, V385K, R387A, A389N, L539E, H540L, M542L, V543H, W189H, W386H, V193G, V193F, V193H, V193K, V193E, V185K/V188R, V188R/P190I and P190I/V193P mutant enzyme in Aspergillus nidulans
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gene-specific expression studies of the AO gene family in melon reveal that only CmAO1 and CmAO4 are transcriptionally active and differentially regulated dependent on tissue, developmental stage and external stimuli, overview
Nicotiana tabacum L., cv. xanthi over-expressing cucumber ascorbate oxidase. In comparison to wild-type plants, leaves of ascorbate oxidase over-expressing plants exhibit reduced stomatal conductance (due to partial stomatal closure), higher water content, and reduced rates of water loss on detachment. Transgenic plants also exhibit elevated levels of hydrogen peroxide and a decline in hydrogen peroxide-scavenging enzyme activity. Leaf abscisic acid content is also higher in ascorbate oxidase over-expressing plants
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overexpression of the enzyme in transgenic tobacco lines
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phylogenetic analysis
quantitative expression analysis
symbiosis-induced AO gene overexpressed in Lotus japonicus during its interaction with either N2-fixing Mesorhizobium loti or the AM fungus Gigaspora margarita, overview