1.10.3.3: L-ascorbate oxidase

This is an abbreviated version!
For detailed information about L-ascorbate oxidase, go to the full flat file.

Word Map on EC 1.10.3.3

Reaction

4 L-ascorbate +

O2
= 4 monodehydroascorbate + 2 H2O

Synonyms

AA oxidase, AA-ox, AAO, AAO1, AAO2, AAO3, Aao4, AO1, AO4, AOase, ASC oxidase, ascorbase, ascorbate dehydrogenase, ascorbate oxidase, ascorbic acid oxidase, ascorbic oxidase, ASOM, L-ascorbate:O2 oxidoreductase, L-ascorbic acid oxidase, oxidase, ascorbate

ECTree

     1 Oxidoreductases
         1.10 Acting on diphenols and related substances as donors
             1.10.3 With oxygen as acceptor
                1.10.3.3 L-ascorbate oxidase

Crystallization

Crystallization on EC 1.10.3.3 - L-ascorbate oxidase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
fully oxidized form of ascorbate oxidase, X-ray diffraction structure determination and analysis at 1.90 A resolution
15 mg/ml enzyme solution, dialysis with 10% 2-methyl-2,4-pentanediol in 50 mM phosphate buffer, pH 5.4 at 4°C, crystals suitable for X-ray studies, 2.5 A resolution
Cucurbita pepo medullosa
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vapour diffusion method, 1.9 M sodium-potassium phosphate buffer pH 7.0 as reservoir solution at 4°C, final protein concentration 5-6 mg/ml
Cucurbita pepo medullosa
-
X-ray crystal structure at 2.5 A resolution
Cucurbita pepo medullosa
-