1.10.3.3: L-ascorbate oxidase

This is an abbreviated version!
For detailed information about L-ascorbate oxidase, go to the full flat file.

Word Map on EC 1.10.3.3

Reaction

4 L-ascorbate +

O2
= 4 monodehydroascorbate + 2 H2O

Synonyms

AA oxidase, AA-ox, AAO, AAO1, AAO2, AAO3, Aao4, AO1, AO4, AOase, ASC oxidase, ascorbase, ascorbate dehydrogenase, ascorbate oxidase, ascorbic acid oxidase, ascorbic oxidase, ASOM, L-ascorbate:O2 oxidoreductase, L-ascorbic acid oxidase, oxidase, ascorbate

ECTree

     1 Oxidoreductases
         1.10 Acting on diphenols and related substances as donors
             1.10.3 With oxygen as acceptor
                1.10.3.3 L-ascorbate oxidase

Engineering

Engineering on EC 1.10.3.3 - L-ascorbate oxidase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A389N
-
slightly reduced thermostability
E177K
-
slightly reduced thermostability
E540L
-
slightly reduced thermostability
H383Y
-
slightly reduced thermostability
L539E
-
slightly increased thermostability
M542L
-
strongly reduced thermostability
P190I
-
reduced thermostability
P190I/V193P
-
pH optimum shifted to pH 4.5, slightly reduced thermostability
Q183R
-
similar properties as wild-type
Q382G
-
slightly higher thermostability
R194Q
-
similar properties as wild-type
R387A
-
slightly reduced thermostability
T527A
-
similar properties as wild-type
V185K
-
slightly reduced thermostability
V185K/V188R
-
similar properties as wild-type
V188R/P190I
-
no detectable activity
V193E
-
pH optimum shifted to pH 4.5, very thermolabile
V193F
-
slightly reduced thermostability
V193G
-
very thermolabile
V193H
-
slightly reduced thermostability
V193K
-
slightly reduced thermostability
V193P
-
pH optimum shifted to pH 5.0
V193T
-
similar properties as wild-type
V385K
-
reduced thermostability
V543H
-
very thermolabile
W189H
-
no detectable activity
W386H
-
reduced thermostability
additional information