L-ascorbate oxidase

This is an abbreviated version!
For detailed information about L-ascorbate oxidase, go to the full flat file.

Word Map on EC


4 L-ascorbate +

= 4 monodehydroascorbate + 2 H2O


AA oxidase, AA-ox, AAO, AAO1, AAO2, AAO3, Aao4, AO1, AO4, AOase, ASC oxidase, ascorbase, ascorbate dehydrogenase, ascorbate oxidase, ascorbic acid oxidase, ascorbic oxidase, ASOM, L-ascorbate:O2 oxidoreductase, L-ascorbic acid oxidase, oxidase, ascorbate


     1 Oxidoreductases
         1.10 Acting on diphenols and related substances as donors
             1.10.3 With oxygen as acceptor
       L-ascorbate oxidase

General Stability

General Stability on EC - L-ascorbate oxidase

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20-25% retention of activity after immobilization, at 12°C, stable for at least 30 days
Cucurbita pepo medullosa
a long acting enzyme derivative is synthesized by covalently linking poly(ethylene glycol) to the enzyme
Cucurbita sp.
ascorbate oxidase is alternately deposited with Au nanoparticles and forms a layer-by-layer membrane on a Pt electrode. The enzyme activities remain even after the deposition. The oxidation current of ascorbic acid (0.1 mM) decreases to 36% for the (ascorbate oxidase/Au)1 modified electrode, and to 19% for the (ascorbate oxidase/Au)10 modified electrode
Cucumis sp.
gelatin, catalase, peroxidase and methemoglobin protect against inactivation
Cucurbita pepo condensa
immobilization within 6% Ca-alginate gel beads improves stability
no change in total activity in the presence of 5 M urea, conversion of tetrameric form into monomer with 75% activity in 8 M urea
Cucurbita pepo medullosa
partially unfolding of the enzyme by 1.4 M guanidinium hydrochloride or 2.8 M urea
role of copper in stability
Cucurbita pepo medullosa
stable to dialysis against EDTA or cyanide