1.10.3.3: L-ascorbate oxidase

This is an abbreviated version!
For detailed information about L-ascorbate oxidase, go to the full flat file.

Word Map on EC 1.10.3.3

Reaction

4 L-ascorbate +

O2
= 4 monodehydroascorbate + 2 H2O

Synonyms

AA oxidase, AA-ox, AAO, AAO1, AAO2, AAO3, Aao4, AO1, AO4, AOase, ASC oxidase, ascorbase, ascorbate dehydrogenase, ascorbate oxidase, ascorbic acid oxidase, ascorbic oxidase, ASOM, L-ascorbate:O2 oxidoreductase, L-ascorbic acid oxidase, oxidase, ascorbate

ECTree

     1 Oxidoreductases
         1.10 Acting on diphenols and related substances as donors
             1.10.3 With oxygen as acceptor
                1.10.3.3 L-ascorbate oxidase

Purification

Purification on EC 1.10.3.3 - L-ascorbate oxidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate fractionation
ammonium sulfate precipitation, DEAE-Sephacel column chromatography, and Sephadex G-100 gel filtration
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ammonium sulfate, DEAE-dextran-silochrome
Cucurbita sp.
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ammonium sulfate, DEAE-Toyopearl, CM-Sephadex, Sephadex G-100
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ammonium sulfate, phenyl Sepharose, Sephacryl S-300, Q-Sepharose, preparative isoelectric focusing, basic and acidic enzyme purified from flour, ammonium sulfate, phenyl Sepharose, Q-Sepharose, Mono S, Superdex 200, basic and acidic enzyme purified from immature kernels
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further purification of different commercial preparations, overview
Cucurbita sp.
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liquid-liquid extraction in aqueous two-phase systems in continuous process using perforated rotating disc contactor with 10% (w/v) PEG 20000 and 25% (w/v) citrate
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native enzyme from cucumber cortex, too homogeneity as blue oxidase
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pre-purification using a aqueous two-phase system (PEG/citrate)
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recombinant wild-type enzyme and various mutants
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