1.10.3.3: L-ascorbate oxidase

This is an abbreviated version!
For detailed information about L-ascorbate oxidase, go to the full flat file.

Word Map on EC 1.10.3.3

Reaction

4 L-ascorbate +

O2
= 4 monodehydroascorbate + 2 H2O

Synonyms

AA oxidase, AA-ox, AAO, AAO1, AAO2, AAO3, Aao4, AO1, AO4, AOase, ASC oxidase, ascorbase, ascorbate dehydrogenase, ascorbate oxidase, ascorbic acid oxidase, ascorbic oxidase, ASOM, L-ascorbate:O2 oxidoreductase, L-ascorbic acid oxidase, oxidase, ascorbate

ECTree

     1 Oxidoreductases
         1.10 Acting on diphenols and related substances as donors
             1.10.3 With oxygen as acceptor
                1.10.3.3 L-ascorbate oxidase

Temperature Stability

Temperature Stability on EC 1.10.3.3 - L-ascorbate oxidase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0 - 40
-
30 min, stable
10
-
half-life: 1047 min
12
Cucurbita pepo medullosa
-
stable for at least 30 days
15
-
immobilized enzyme retains full activity for 3 months at pH 5.0-7.0, free enzyme looses 40-70% activity within one day at pH 5.0-7.0
40
-
stable for 30 min
40 - 50
50
-
stable for 5 min
50 - 80
-
during thermal treatments for 10 min, AAO in broccoli florets and stalks is stable until around 50°C. A 10 min thermal treatment at 80°C almost completely inactivates AAO. AAO inactivation is irreversible since no increase in activity is observed when extracts of thermal treated broccoli are stored at 4°C for 24 h
55
Cucurbita sp.
-
no loss in activity after 30 min, approx. 55% activity after 30 min at 60°C, approx. 30% activity after 30 min at 65°C
70
-
half-life: 21.2 min
90
-
thermal inactivation of AAO follows first-order reaction kinetics. Heating at temperatures above 90°C for short times results in a complete AAO inactivation, resulting in a protective effect of L-ascorbic acid toward enzyme-catalyzed oxidation
additional information