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1.11.1.10: chloride peroxidase

This is an abbreviated version!
For detailed information about chloride peroxidase, go to the full flat file.

Word Map on EC 1.11.1.10

Reaction

RH
+
chloride
 +
H2O2
=
RCl
 + 2 H2O

Synonyms

CCPO, Chloride peroxidase, chloroperoxidase, chloroproxidase, CPO, CPO-I, CPO2, haeme-thiolate peroxidase, heme-containing CPO, heme-thiolate chloroperoxidase, More, peroxidase, chloride, Vanadium chloride peroxidase, vCPO

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.10 chloride peroxidase

Storage Stability

Storage Stability on EC 1.11.1.10 - chloride peroxidase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10°C, pH 6.0, half-life of autoxidation of the oxygenated heme protein is 20 min
-
-20°C, pH 6, less than 20% loss of activity after 6 months
-
-20°C, pH 6.0, half-life of autoxidation of the oxygenated heme protein is 85 min
-
4°C, citrate buffer (50 mM, pH 3.0), 4 weeks, 91% loss of activity of the soluble enzyme, immobilized (covalent immobilization of chloroperoxidase on the magnetic p(GMA-MMA-EGDMA) beads) enzyme loses 15% of its initial activity
-
4°C, conjugated-CPO in citrate buffer (50 mM, pH 3.0), 8 weeks, the conjugated-CPO preserves its initial activity more than 60%
-
4°C, CPO immobilized into glutaraldehyde-3-aminopropyltrimethoxysilane-SBA-15 in a buffer at pH 3.4, storage results in leaching of the enzyme from the support due to hydrolytic cleavage of the imino bond
-
4°C, CPO immobilized into glutaraldehyde-3-aminopropyltrimethoxysilane-SBA-15 in a buffer at pH 7.0, a few days, complete loss of enzyme activity
-
4°C, free and conjugated-CPO in citrate buffer (50 mM, pH 3.0), 4 weeks, the activity loss of the conjugated-CPO is 7% while the free enzyme loses its initial activity about 83%
-
4°C, free enzyme in citrate buffer (50 mM, pH 3.0), 6 weeks, the free enzyme loses all its activity
-