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1.11.1.11: L-ascorbate peroxidase

This is an abbreviated version!
For detailed information about L-ascorbate peroxidase, go to the full flat file.

Word Map on EC 1.11.1.11

Reaction

2 L-ascorbate +

H2O2
+ 2 H+ = 2 monodehydroascorbate + 2 H2O

Synonyms

Am-pAPX1, APEX2, APOX, APX, APX 1, APX 2, APX1, APX2, APX3, APX4, APX5, APX6, APX7, APx8, APXS, APXT, ascorbate peroxidase, ascorbate peroxidase 1, ascorbate peroxidase 2, ascorbate peroxidase 3, ascorbate peroxidase 4, ascorbate peroxidase 5, ascorbate peroxidase 6, ascorbate peroxidase 7, ascorbate peroxidase 8, ascorbate peroxidase6, ascorbic acid peroxidase, At1g07890, AT1G77490, AT3G09640, AT4G08390, AT4G32320, AT4G35000, AT4G35970, AtAPx08, AtAPX1, AtAPX2, AtAPX3, AtAPX5, AtAPX6, AtSAPX, AtstAPX, AtTAPX, cAPX, cAPX 2, CmstAPX, CrAPX4, CreAPX1, CreAPX2, CreAPX4, CreAPXheme, CsAPX1, cytoplasmic ascorbate peroxidase 1, cytosolic ascorbate peroxidase, GhAPX1, glyoxysomal APX, HvAPX1, L-ascorbate peroxidase, L-ascorbate peroxidase 3, L-ascorbate peroxidase 5, L-ascorbate peroxidase 6, L-ascorbate peroxidase, heme-containing, L-ascorbic acid peroxidase, L-ascorbic acid-specific peroxidase, LmAPX, MaAPX1, OsAPx1, OsAPx2, OsAPx3, OsAPx4, OsAPx5, OsAPx6, OsAPx7, OsAPx8, OsAPXa, OsAPXb, pAPX, Pavirv00022559m, peroxidase, ascorbate, peroxisomal ascorbate peroxidase, PgAPX1, PHYPA_001206, PHYPA_001884, PHYPA_021776, PHYPA_024580, PHYPA_024582, Potri.002G081900, Potri.004G174500, Potri.005G112200, Potri.005G161900, Potri.005G179200, Potri.006G089000, Potri.006G132200, Potri.006G254500, Potri.009G015400, Potri.009G134100, Potri.016G084800, PpAPX, PpAPX-S, PpAPX2, PpAPX2.1, PpAPX2.2, PpAPX3, PpAPX6-related, PtAPX-S.1, PtAPX-S.2, PtAPX-TL29, PtAPX.3, PtAPX1.1, PtAPX1.2, PtAPX2, PtAPX3, PtAPX5, PtAPX5-like, PtAPX6 related, PtotAPX, RcAPX, sAPX, stromal APX, stromal ascorbate peroxidase, stromal ascorbate peroxidases, TaAPX, tAPX, thylakoid ascorbate peroxidase, thylakoid membrane-bound ascorbate peroxidases, thylakoid-bound ascorbate peroxidase

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.11 L-ascorbate peroxidase

Cloned

Cloned on EC 1.11.1.11 - L-ascorbate peroxidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
APX is comprised of different isoenzymes, which are encoded by a multi-gene family APX1 DNA and amino acid sequence analysis, sequence comparisons and phylogenetic analysis and tree, conserved cis-regulatory elements in the promoters of the APX isozyme, transcriptional profiles of the rice isozymes, overview
APX is comprised of different isoenzymes, which are encoded by a multi-gene family, APX2 DNA and amino acid sequence analysis, sequence comparisons and phylogenetic analysis and tree, conserved cis-regulatory elements in the promoters of the APX isozyme, transcriptional profiles of the rice isozymes, overview
APX is comprised of different isoenzymes, which are encoded by a multi-gene family, APX3 DNA and amino acid sequence analysis, sequence comparisons and phylogenetic analysis and tree, conserved cis-regulatory elements in the promoters of the APX isozyme, transcriptional profiles of the rice isozymes, overview
APX is comprised of different isoenzymes, which are encoded by a multi-gene family, APX4 DNA and amino acid sequence analysis, sequence comparisons and phylogenetic analysis and tree, conserved cis-regulatory elements in the promoters of the APX isozyme, transcriptional profiles of the rice isozymes, overview
APX is comprised of different isoenzymes, which are encoded by a multi-gene family, APX5 DNA and amino acid sequence analysis, sequence comparisons and phylogenetic analysis and tree, conserved cis-regulatory elements in the promoters of the APX isozyme, transcriptional profiles of the rice isozymes, overview
APX is comprised of different isoenzymes, which are encoded by a multi-gene family, APX6 DNA and amino acid sequence analysis, sequence comparisons and phylogenetic analysis and tree, conserved cis-regulatory elements in the promoters of the APX isozyme, transcriptional profiles of the rice isozymes, overview
APX is comprised of different isoenzymes, which are encoded by a multi-gene family, APX7 DNA and amino acid sequence analysis, sequence comparisons and phylogenetic analysis and tree, conserved cis-regulatory elements in the promoters of the APX isozyme, transcriptional profiles of the rice isozymes, overview
APX is comprised of different isoenzymes, which are encoded by a multi-gene family, APX8 DNA and amino acid sequence analysis, sequence comparisons and phylogenetic analysis and tree, conserved cis-regulatory elements in the promoters of the APX isozyme, transcriptional profiles of the rice isozymes, overview
cotransformation of a soybean cDNA library and the Bax gene into yeast cells, screening for expressed genes that prevent Bax-induced apoptosis, the soybean ascorbate peroxidase inhibits the generation of reactive oxygen species by Bax, which in turn suppresses Bax-induced cell death in yeast
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DNA and amino acid sequence analysis, sequence comparisons and phylogenetic analysis and tree, conserved cis-regulatory elements in the promoters of the APX isozyme, overview
enzyme expression analysis, phylogenetic analysis and tree
enzyme expression analysis, phylogenetic analysis and tree, in Blastocrithidia sp. P57 very low APX activity is detected, despite the fact that the species apparently lacks the corresponding gene
Blastochritidia sp. P57
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enzyme expression analysis, phylogenetic analysis and tree, in Rhynchopus humris very low APX activity is detected, despite the fact that the species apparently lacks the corresponding gene
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enzyme PtotAPX expression analysis in callus cultures, recombinant expression of GFP-tagged enzyme in tobacco plants, localization to the chloroplasts
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expressed in Escherichia coli strain BL21 Star
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expression as a fusion product with the Escherichia coli maltose-binding protein
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expression in Escherichia coli
expression in Escherichia coli of two soybean ascorbate peroxidase cDNAs
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expression in Escherichia coli, most of the enzyme produced is present in the apo-form, without heme
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expression in Escherichia coli, the presence of 3% NaCl as well as beta-D-thiogalactopyranoside is needed for the expression
Chlamydomonas sp.
expression in Escherichia coli. Expression of holo enzyme depends on the simultaneous production of protein and heme by the bacteria
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expression in Saccharomyces cerevisiae
expression of an epitope-tagged form of the enzyme in Trypanosoma cruzi
full length AtstAPX is cloned into the pPZP221 binary vector, introduced into Arabidopsis thaliana by the floral dip method using Agrobacterium tumefaciens strain GV3101, for the generation of antibodies into the vector pQE80L for expression in Escherichia coli cells
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full length CmstAPX is cloned into the pPZP221 binary vector, introduced into Arabidopsis thaliana by the floral dip method using Agrobacterium tumefaciens strain GV3101, for the generation of antibodies into the vector pQE80L for expression in Escherichia coli cells
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gee APX, DNA and amino acid sequence analysis, sequence comparisons and phylogenetic analysis and tree, conserved cis-regulatory elements in the promoters of the APX isozyme, overview
gene AgAPX1, DNA and amino acid sequence determination and analysis, phylogenetic tree, recombinant expression in Escherichia coli strain BL21(DE3), recombinant GFP-tagged enzyme expression in onion epidermal cells for analysis of subcellular localization of AgAPX1. Overexpression of AgAPX1 in Arabidopsis thaliana positively regulates drought tolerance by regulating the stomata aperture. Net photosynthetic rate considerably decreases in the wild-type compared to transgenic lines under drought stress
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gene APX, DNA and amino acid sequence analysis, sequence comparisons and phylogenetic analysis and tree, conserved cis-regulatory elements in the promoters of the APX isozyme, overview
gene APX, DNA and amino acid sequence determination and analysis. The 1938 bp RcAPX gene encompasses 6 introns and 7 exons. The open reading frame of RcAPX is 750 bp long and encodes a 249-amino acid peptide, sequence comparisons and phylogenetic analysis and tree, quantitative real-time PCR enzyme expression analysis. Recombinant expression in Escherichia coli
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gene APX, stable ectopic expression of His-V5-tagged or c-Myc-/GFP-tagged cAPX protein from a CMV-V5-His plasmid in mouse fibroblast-like cell line C3H10T1/2. Confirmation of cell transfection with this plasmid is performed by western blotting using anti-Myc antibody and by fluorescence microscopy. Method, overview
gene APX1, DNA and amino acid sequence analysis, sequence comparisons and phylogenetic analysis and tree, conserved cis-regulatory elements in the promoters of the APX isozyme, overview
gene APX1, overexpression CsAPX1 in Arabidopsis thaliana
gene APX1, quantitative realtime-PCR isozyme expression analysis
gene APX2, quantitative realtime-PCR isozyme expression analysis
gene APX3, quantitative realtime-PCR isozyme expression analysis
gene APX4, quantitative realtime-PCR isozyme expression analysis
gene APX5, quantitative realtime-PCR isozyme expression analysis
gene APX6, quantitative realtime-PCR isozyme expression analysis
gene APX6, quantitative RT-PCR enzyme expression analysis, transient expression assays in Nicotiana benthamiana leaves, constitutive expression of APX6 is restricted to old and dying cells and absent in younger tissues, thus age-dependent post-transcriptional regulation of APX6. The coding sequence of APX6 is a potential target of miR398, which is a key regulator of copper redistribution
gene APX7, quantitative realtime-PCR isozyme expression analysis
gene APX8, quantitative realtime-PCR isozyme expression analysis
gene AtAPX1, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene DsAPX, DNA and amino acid sequence determination and analysis, sequence comparisons, quantitative RT-PCR enzyme expression analysis
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gene MaAPX1, phenotype and genotyping, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative RT-PCR expression analysis of MaMsrB2 and MaAPX1 genes. Recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), coexpression of gene MsrB2 as His-tagged enzyme. Ectopic recombinant overexpression of MaAPX1 in Arabidopsis thaliana leading to delay of the detached leaf senescence induced by dark in Arabidopsis
GhAPX1 is expressed in Escherichia coli
into the pBI121 binary vector for a Agrobacterium tumefaciens-mediated tobacco leaf disc transformation, a CaMV 35S promoter and a rd29A promoter driven construct is used
into the vector pET-28a+ for the expression in Escherichia coli BL21DE3 cells, the 5'-flanking region of the PgAPX1 gene is cloned into the Topo-TA vector
into the vector pGEM-T Easy for sequencing
into the vector pXG B2863 for transfection of Leishmania major cells
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into the vector pXG-B2863 for transfection of Leishmania major cells
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overexpression in Arabidopsis. Transgenic lines over-expressing OsAPXb show higher salt tolerance than OsAPXa transgenic lines. Overproduction of OsAPXb enhances and maintains APX activity to a much higher degree than OsAPXa in transgenic Arabidopsis during treatment with different concentrations of NaCl, enhances the active oxygen scavenging system, and protects plants from salt stress by equilibrating H2O2 metabolism
overexpression in Arabidopsis. Transgenic lines over-expressing OsAPXb showed higher salt tolerance than OsAPXa transgenic lines. Overproduction of OsAPXb enhances and maintains APX activity to a much higher degree than OsAPXa in transgenic Arabidopsis during treatment with different concentrations of NaCl, enhances the active oxygen scavenging system, and protects plants from salt stress by equilibrating H2O2 metabolism
overexpression in Escherichia coli
recombinant enzyme
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the entire and truncated versions of APX (1-140, 1-250, 140-439, 250-439 and 140-250) are cloned into pGADT7 to fuse to the GAL4 activation domain and expressed in yeast
the vector pT-7 is used, cytochrome c peroxidase, CCP, is converted into an ascorbate peroxidase, APX, by engineering the ascorbate-binding loop and critical arginine into CCP to give the CCP2APX mutant
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transgenic Arabidopsis thaliana constitutively overexpressing HvAPX1 under control of the CaMv 35S promoter
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