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1.11.1.11: L-ascorbate peroxidase

This is an abbreviated version!
For detailed information about L-ascorbate peroxidase, go to the full flat file.

Word Map on EC 1.11.1.11

Reaction

2 L-ascorbate +

H2O2
+ 2 H+ = 2 monodehydroascorbate + 2 H2O

Synonyms

Am-pAPX1, APEX2, APOX, APX, APX 1, APX 2, APX1, APX2, APX3, APX4, APX5, APX6, APX7, APx8, APXS, APXT, ascorbate peroxidase, ascorbate peroxidase 1, ascorbate peroxidase 2, ascorbate peroxidase 3, ascorbate peroxidase 4, ascorbate peroxidase 5, ascorbate peroxidase 6, ascorbate peroxidase 7, ascorbate peroxidase 8, ascorbate peroxidase6, ascorbic acid peroxidase, At1g07890, AT1G77490, AT3G09640, AT4G08390, AT4G32320, AT4G35000, AT4G35970, AtAPx08, AtAPX1, AtAPX2, AtAPX3, AtAPX5, AtAPX6, AtSAPX, AtstAPX, AtTAPX, cAPX, cAPX 2, CmstAPX, CrAPX4, CreAPX1, CreAPX2, CreAPX4, CreAPXheme, CsAPX1, cytoplasmic ascorbate peroxidase 1, cytosolic ascorbate peroxidase, GhAPX1, glyoxysomal APX, HvAPX1, L-ascorbate peroxidase, L-ascorbate peroxidase 3, L-ascorbate peroxidase 5, L-ascorbate peroxidase 6, L-ascorbate peroxidase, heme-containing, L-ascorbic acid peroxidase, L-ascorbic acid-specific peroxidase, LmAPX, MaAPX1, OsAPx1, OsAPx2, OsAPx3, OsAPx4, OsAPx5, OsAPx6, OsAPx7, OsAPx8, OsAPXa, OsAPXb, pAPX, Pavirv00022559m, peroxidase, ascorbate, peroxisomal ascorbate peroxidase, PgAPX1, PHYPA_001206, PHYPA_001884, PHYPA_021776, PHYPA_024580, PHYPA_024582, Potri.002G081900, Potri.004G174500, Potri.005G112200, Potri.005G161900, Potri.005G179200, Potri.006G089000, Potri.006G132200, Potri.006G254500, Potri.009G015400, Potri.009G134100, Potri.016G084800, PpAPX, PpAPX-S, PpAPX2, PpAPX2.1, PpAPX2.2, PpAPX3, PpAPX6-related, PtAPX-S.1, PtAPX-S.2, PtAPX-TL29, PtAPX.3, PtAPX1.1, PtAPX1.2, PtAPX2, PtAPX3, PtAPX5, PtAPX5-like, PtAPX6 related, PtotAPX, RcAPX, sAPX, stromal APX, stromal ascorbate peroxidase, stromal ascorbate peroxidases, TaAPX, tAPX, thylakoid ascorbate peroxidase, thylakoid membrane-bound ascorbate peroxidases, thylakoid-bound ascorbate peroxidase

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.11 L-ascorbate peroxidase

Substrates Products

Substrates Products on EC 1.11.1.11 - L-ascorbate peroxidase

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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 L-ascorbate + H2O2 + 2 H+
2 monodehydroascorbate + 2 H2O
show the reaction diagram
2 L-ascorbate + H2O2 + 2 H+
L-ascorbate + L-dehydroascorbate + 2 H2O
show the reaction diagram
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+
? + H2O
show the reaction diagram
-
-
-
-
?
2,2'-azino-di-(3-ethyl-benzothiazoline-(6)-sulfonic acid) + H2O2
? + H2O
show the reaction diagram
-
3% relative activity to L-ascorbate
-
?
2,2'-azino-di-[3-ethylbenzothiazoline-(6)-sulfonic acid] + H2O2
?
show the reaction diagram
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
?
show the reaction diagram
-
cytosolic ascorbate peroxidase shows 3% activity compared to L-ascorbate, in the presence of 0.1 mM H2O2 and 3.6% activity compared to L-ascorbate in the presence of 0.5 mM H2O2
-
-
?
cysteine + H2O2
? + H2O
show the reaction diagram
-
enzyme partially purified from whole body homogenate, 40% of the activity with L-ascorbate
-
?
Cytochrome c + H2O
?
show the reaction diagram
-
-
-
-
?
cytochrome c + H2O2
?
show the reaction diagram
-
able to use both ascorbate and cytochrome c as reducing electron donors
-
-
?
cytochrome c + H2O2
? + H2O
show the reaction diagram
D-araboascorbic acid + H2O2
dehydroascorbate + H2O
show the reaction diagram
-
56% activity relative to L-ascorbate
-
?
D-iso-ascorbate + H2O2
dehydroascorbate + H2O
show the reaction diagram
Chlamydomonas sp.
native enzyme: the activity with D-isoascorbate corresponds to 131% of that found with ascorbate, recombinant enzyme: the activity with D-isoascorbate corresponds to 129% of that found with ascorbate
-
?
D-isoascorbate + H2O2
?
show the reaction diagram
-
60.3% activity compared to L-ascorbate
-
-
?
dihydrorhodamine 123 + H2O2
?
show the reaction diagram
ethyl phenyl sulfide + H2O2
? + H2O
show the reaction diagram
-
-
-
-
?
ferrocyanide + H2O2
ferricyanide + H2O
show the reaction diagram
-
the Cys32Ser mutation has little effect on the kinetics of ferrocyanide turnover, but the DTNB modification decreases activity by approximately 90% at 300 mM ferrocyanide
-
?
glutathione + H2O2
? + H2O
show the reaction diagram
GSSG + H2O2
?
show the reaction diagram
about 20% of the activity with L-ascorbate
-
-
?
guaiacol + H2O2
?
show the reaction diagram
guaiacol + H2O2
? + H2O
show the reaction diagram
iodide + H2O2
?
show the reaction diagram
-
2.3% activity relative to L-ascorbate
-
?
isopropyl phenyl sulfide + H2O2
? + H2O
show the reaction diagram
-
-
-
?
L-ascorbate + cumene hydroperoxide
?
show the reaction diagram
-
8.0% activity compared to H2O2
-
-
?
L-ascorbate + H2O2
? + H2O
show the reaction diagram
-
-
-
-
?
L-ascorbate + H2O2
dehydroascorbate + 2 H2O
show the reaction diagram
L-ascorbate + H2O2
dehydroascorbate + H2O
show the reaction diagram
L-ascorbate + tert-butyl hydroperoxide
?
show the reaction diagram
-
17.4% activity compared to H2O2
-
-
?
L-ascorbic acid + cumene hydroperoxide
dehydroascorbate + 1,1-dimethylbenzylalcohol + H2O
show the reaction diagram
L-ascorbic acid + tert-butylhydroperoxide
dehydroascorbate + tert-butylalcohol
show the reaction diagram
methyl naphthalene sulfide + H2O2
? + H2O
show the reaction diagram
-
-
-
?
methyl phenyl sulfide + H2O2
? + H2O
show the reaction diagram
-
-
-
?
n-propyl phenyl sulfide + H2O2
? + H2O
show the reaction diagram
-
-
-
?
NADH + H+ + H2O2
NAD+ + H2O
show the reaction diagram
-
10% of the activity with ascorbate, APX 1, 1% of the activity with ascorbate, APX 2
-
-
?
NADPH + H+ + H2O2
NADP+ + H2O
show the reaction diagram
-
27% of the activity with ascorbate, APX 1, 21% of the activity with ascorbate, APX 2
-
-
?
NADPH + H2O2
? + H2O
show the reaction diagram
o-dianisidine + H2O2
?
show the reaction diagram
p-chlorophenyl methyl sulfide + H2O2
? + H2O
show the reaction diagram
-
-
-
?
p-cresol + cumene-hydroperoxide
4a,9b-dihydro-8,9b-dimethyl-3(4H)-dibenzofuranone + 2,2'-dihydroxy-5,5'-dimethylbiphenyl + 1,1-dimethylbenzylalcohol + bis-(1-methyl-1-phenylethyl)peroxide
show the reaction diagram
-
-
the formation of bis-(1-methyl-1-phenylethyl)peroxide derives from the reaction of 1,1-dimethylbenzylalcohol with either p-cresol or 2,2'-dihydroxy-5,5'-dimethylbiphenyl
?
p-cresol + H2O2
4a,9b-dihydro-8,9b-dimethyl-3(4H)-dibenzofuranone + 2,2'-dihydroxy-5,5'-dimethylbiphenyl + H2O
show the reaction diagram
-
-
these products, which are derived from reactions of the p-methylphenoxy radical, itself form as a direct result of single-electron oxidation of p-cresol by the enzyme, can be accommodated from the known chemistry of the radical products, the product ratio 4alpha,9beta-dihydro-8,9beta-dimethyl-3(4H)-dibenzofuranone: 2,2'-dihydroxy-5,5'-dimethylbiphenyl is found to depend on enzyme concentration
?
p-nitrophenyl methyl sulfide + H2O2
? + H2O
show the reaction diagram
-
-
-
?
pyrocatechol + H2O2
1,2-benzoquinone + H2O
show the reaction diagram
-
low activity compared to L-ascorbate
-
?
pyrogallol + H2O2
3-hydroxybenzo-1,2-quinone + H2O
show the reaction diagram
pyrogallol + H2O2
?
show the reaction diagram
pyrogallol + H2O2
? + H2O
show the reaction diagram
-
32% of the activity with ascorbate
-
-
?
reductic acid + H2O2
?
show the reaction diagram
-
i.e. 2,3-dihydroxy-2-cyclopenten-1-one, 7.1% activity relative to L-ascorbate
-
?
additional information
?
-