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0.04
-
effect of dialysis on MnP recovered from solid-state culture at 10°C, dialyzed extract, extraction conducted for 3h at 100 rpm, U/mg of fugal biomass, pH 4.0
0.1
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specific MnP activity recovered from solid-state culture after 3h and 10°C, U/mg of fugal biomass, pH 6.0
0.12
-
specific MnP activity recovered from solid-state culture after 3h and 25°C, U/mg of fugal biomass, pH 6.0
0.16
-
effect of dialysis on MnP recovered from solid-state culture at 10°C, dialyzed extract, extraction conducted for 1h without agitation, U/mg of fugal biomass, pH 5.0
0.2
-
specific MnP activity recovered from solid-state culture after 1h and 25°C, U/mg of fugal biomass, pH 4.0
0.21
-
effect of dialysis on MnP recovered from solid-state culture at 10°C, dialyzed extract, extraction conducted for 3h at 100 rpm, U/mg of fugal biomass, pH 5.0
0.27
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effect of dialysis on MnP recovered from solid-state culture at 10°C, dialyzed extract, extraction conducted for 1h without agitation, U/mg of fugal biomass, pH 4.0
0.45
-
specific MnP activity recovered from solid-state culture after 1h and 25°C, U/mg of fugal biomass, pH 5.0
0.48
-
specific MnP activity recovered from solid-state culture after 3h and 25°C, U/mg of fugal biomass, pH 4.0
0.7
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specific MnP activity recovered from solid-state culture after 3h and 25°C, U/mg of fugal biomass, pH 5.0
10.2
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oxidation of 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate in presence of Mn2+ and H2O2
101.85
-
purified enzyme from strain NITW715076_1, pH 5.0, 60°C
112.4
purified enzyme, pH 4.8, 30°C
1230
-
purification step CIM QA disk 3, chromatographic MnP form 3, CIM3
169
-
purification step chromatography on a concanavalin-A Sepharose column
17.2
-
recombinant protein, pH 4.5, 37°C
180
-
Mn3+-lactate complex formation
185
-
purification step crude medium
2250
-
purification step Mono Q2
23.8
pH 4.5, 30°C, purified recombinant refolded His6-tagged enzyme
241.18
-
purified enzyme from strain NITW715076, pH 5.0, 60°C
2480
-
purification step CIM QA disk 1, chromatographic MnP form 1, CIM1
3430
-
purification step CIM QA disk 2, chromatographic MnP form 2, CIM2
41
-
purification step ultrafiltration
65 - 134
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depending on culture age
660
-
o-toluidine as substrate for Mn3+-oxidation
733
-
purification step DEAE Sepharose
84.08
-
purified enzyme from strain NITW715076_2, pH 5.0, 60°C
870
-
purification step Mono Q1
0.01
-
specific MnP activity recovered from solid-state culture after 1h and 10°C, U/mg of fugal biomass, pH 6.0
0.01
-
specific MnP activity recovered from solid-state culture after 1h and 25°C, U/mg of fugal biomass, pH 6.0
0.31
-
effect of dialysis on MnP recovered from solid-state culture at 10°C, crude extract, extraction conducted for 3h at 100 rpm, U/mg of fugal biomass, pH 4.0
0.31
-
specific MnP activity recovered from solid-state culture after 3h and 10°C, U/mg of fugal biomass, pH 4.0
0.35
-
effect of dialysis on MnP recovered from solid-state culture at 10°C, crude extract, extraction conducted for 1h without agitation, U/mg of fugal biomass, pH 4.0
0.35
-
effect of dialysis on MnP recovered from solid-state culture at 10°C, crude extract, extraction conducted for 1h without agitation, U/mg of fugal biomass, pH 5.0
0.35
-
specific MnP activity recovered from solid-state culture after 1h and 10°C, U/mg of fugal biomass, pH 4.0
0.35
-
specific MnP activity recovered from solid-state culture after 1h and 10°C, U/mg of fugal biomass, pH 5.0
0.41
-
effect of dialysis on MnP recovered from solid-state culture at 10°C, crude extract, extraction conducted for 3h at 100 rpm, U/mg of fugal biomass, pH 5.0
0.41
-
specific MnP activity recovered from solid-state culture after 3h and 10°C, U/mg of fugal biomass, pH 5.0
288
-
purification step gel filtration
288
-
after 7.2fold purification, pH 5.5, 45°C
40
-
purification step culture supernatant
40
-
culture supernatant, pH 5.5, 45°C
additional information
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enzyme yield is species-dependent and strain-dependent, the carbon source and lignocellulosic substrate play a crucial role in enzyme production
additional information
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-
additional information
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additional information
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enzyme yield is species-dependent and strain-dependent, the carbon source and lignocellulosic substrate play a crucial role in enzyme production
additional information
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-
additional information
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enzyme yield is species-dependent and strain-dependent, the carbon source and lignocellulosic substrate play a crucial role in enzyme production
additional information
Deuteromycotina sp.
-
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additional information
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enzyme yield is species-dependent and strain-dependent, the carbon source and lignocellulosic substrate play a crucial role in enzyme production
additional information
oxidative activity for phenols/non-phenols by recombinant isozyme Il-MnP1 in the presence/absence of Mn2+ in U/l, overview. For monophenols (DMP, guaiacol) without Mn2+, the reaction results in a 95% reduction of oxidative activity, whereas bisphenols are only found to obtain a 20% reduction (catechol) or 50% increase (HQ). As for non-phenols, veratryl alcohol is oxidized only when Mn2+ is present, while Il-MnP1 still retains 70% of its oxidative activity toward ABTS in the absence of Mn2+
additional information
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oxidative activity for phenols/non-phenols by recombinant isozyme Il-MnP1 in the presence/absence of Mn2+ in U/l, overview. For monophenols (DMP, guaiacol) without Mn2+, the reaction results in a 95% reduction of oxidative activity, whereas bisphenols are only found to obtain a 20% reduction (catechol) or 50% increase (HQ). As for non-phenols, veratryl alcohol is oxidized only when Mn2+ is present, while Il-MnP1 still retains 70% of its oxidative activity toward ABTS in the absence of Mn2+
additional information
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maximum MnP activity for strain NITW715076_2 is 92.77 U/ml at 50°C. For strains NITW715076_1 and NITW715076, the MnP activity is 145.65 U/ml1 at 70°C and 107.24 U/ml1 at 60°C, respectively
additional information
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66.6, 1 unit: oxidation of 3,3,5,5-tetramethylbenzidine so that the change in A650 is 0.01 per min
additional information
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-
additional information
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-
additional information
-
-
additional information
-
-
additional information
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216-467, 1 unit: initial increase in absorbance of 1.0 per min at 465 nm
additional information
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216-467, 1 unit: initial increase in absorbance of 1.0 per min at 465 nm
additional information
0.055 U/ml, R42A mutant, in the presence of 2,6-dimethoxyphenol
additional information
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0.055 U/ml, R42A mutant, in the presence of 2,6-dimethoxyphenol
additional information
0.069 U/ml, N131D mutant, in the presence of 2,6-dimethoxyphenol
additional information
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0.069 U/ml, N131D mutant, in the presence of 2,6-dimethoxyphenol
additional information
0.076 U/ml, wild-type enzyme, in the presence of 2,6-dimethoxyphenol
additional information
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0.076 U/ml, wild-type enzyme, in the presence of 2,6-dimethoxyphenol
additional information
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425 U/l of MnP is extracted from the liquid fermentation medium of Phanerochaete chrysosporium and exhibits an efficient catalytic performance for SMX transformation. Optimal activity when the external parameters are designed at pH 5.0, an enzyme activity above 40 U/l, and an H2O2 concentration of 0.2 mM
additional information
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additional information
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additional information
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additional information
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screening of enzyme activity duirng optimization of enzyme production method from Pseudolagarobasidium sp. PP17-33, overview
additional information
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enzyme yield is species-dependent and strain-dependent, the carbon source and lignocellulosic substrate play a crucial role in enzyme production
additional information
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340-350 U/l
additional information
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enzyme yield is species-dependent and strain-dependent, the carbon source and lignocellulosic substrate play a crucial role in enzyme production
additional information
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enzyme yield is species-dependent and strain-dependent, the carbon source and lignocellulosic substrate play a crucial role in enzyme production
additional information
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284-422: 5 isozymes, expressed as spectral change in DELTAA per min and mg of heme-containing protein
additional information
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enzyme yield is species-dependent and strain-dependent, the carbon source and lignocellulosic substrate play a crucial role in enzyme production
additional information
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340-350 U/l
additional information
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enzyme yield is species-dependent and strain-dependent, the carbon source and lignocellulosic substrate play a crucial role in enzyme production