1.11.1.14: lignin peroxidase

This is an abbreviated version, for detailed information about lignin peroxidase, go to the full flat file.

Reaction

1-(3,4-dimethoxyphenyl)-2-(2-methoxyphenoxy)propane-1,3-diol
+
H2O2
=
3,4-dimethoxybenzaldehyde
+
2-methoxyphenol
+
glycolaldehyde
+
H2O

Synonyms

ALiP-P3, diarylpropane oxygenase, diarylpropane:oxygen,hydrogen-peroxide oxidoreductase (C-C-bond-cleaving), DypB, Glg4, H2O2-dependent ligninase, heme-containing lignin peroxidase, heme-containing peroxidase, lignin peroxidase, lignin peroxidase LIII, ligninase, ligninase H2, ligninase H8, ligninase I, ligninase LG5, LIP, Lip1, LIP2, lipJ, mushroom tyrosinase, oxygenase, diarylpropane, Pr-lip1, Pr-lip4

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.14 lignin peroxidase

Purification

Purification on EC 1.11.1.14 - lignin peroxidase

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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
10 kDa ultrafiltration, 3 M ammonium sulphate precipitation, hydrophobic interaction chromatography, reversed phase chromatography
-
16 isozymes: TvLP1-16, differing in primary structure
-
3 isozymes by ion-exchange chromatography
-
at least 7 isozymes, LiP-1-7
-
DEAE-cellulose column chromatography
-
ion exchange chromatography, 4°C
-
purification from culture supernatant
-
Resource Q column chromatography, gel filtration
-
salt precipitation with 60% (NH4)2SO4, desalting column, Q FF ion exchange column and Sepharyl S-300 HR gel filtration
-
to homogeneity using Amicon concentration and DEAE cellulose chromatography
Loweporus lividus
-
ultrafiltration, anion-exchange and size exclusion chromatography
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