1.11.1.14: lignin peroxidase

This is an abbreviated version, for detailed information about lignin peroxidase, go to the full flat file.

Reaction

1-(3,4-dimethoxyphenyl)-2-(2-methoxyphenoxy)propane-1,3-diol
+
H2O2
=
3,4-dimethoxybenzaldehyde
+
2-methoxyphenol
+
glycolaldehyde
+
H2O

Synonyms

ALiP-P3, diarylpropane oxygenase, diarylpropane:oxygen,hydrogen-peroxide oxidoreductase (C-C-bond-cleaving), DypB, Glg4, H2O2-dependent ligninase, heme-containing lignin peroxidase, heme-containing peroxidase, lignin peroxidase, lignin peroxidase LIII, ligninase, ligninase H2, ligninase H8, ligninase I, ligninase LG5, LIP, Lip1, LIP2, lipJ, mushroom tyrosinase, oxygenase, diarylpropane, Pr-lip1, Pr-lip4

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.14 lignin peroxidase

Temperature Stability

Temperature Stability on EC 1.11.1.14 - lignin peroxidase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10 - 70
-
temperature range preserving 70-100% of initial activity, pH 5.0, 1 h
10 - 40
10 - 70
-
temperature range preserving 70-100% of initial activity, pH 5.0, 1 h
10 - 40
-
temperature range preserving 70-100% of initial activity, pH 5.0, 1 h
23
-
retains nearly 30% of initial activity after 18 h, in presence of veratryl alcohol
25 - 75
-
pH 5, 100% lignin peroxidase activity is retained at 55°C after incubation. The lignin peroxidase activity drops more than 75% at temperatures below 35°C and loses about 66% of its activity at a temperature of 75°C
25
-
pH 7.0, 4 h, maximum activity loss of 10%
30 - 60
-
the recombinant enzyme is fully stable up to 30°C but unstable at 37°C, and is completely inactivated after 30 min at 60°C
40
21 h, pH 4.0-6.5, 80% residual activity
45
-
after 2 h incubation at 45°C, 55% of the initial activity of the immobilized lignin peroxidase (on nanoporous gold) is still retained while the free lignin peroxidase is completely deactivated, pH 3.5 citrate buffer
50
stable for 48 h up to 50°C
60
6 h, complete loss of activity
70
min, complete loss of activity