1.11.1.19: dye decolorizing peroxidase
This is an abbreviated version!
For detailed information about dye decolorizing peroxidase, go to the full flat file.
Word Map on EC 1.11.1.19
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1.11.1.19
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peroxidases
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heme
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lignin
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auricula-judae
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thanatephorus
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cucumeris
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veratryl
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auricularia
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irpex
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ligninolytic
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lacteus
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lignin-degrading
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kraft
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adusta
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bjerkandera
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chlorite
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nonphenolic
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2,6-dimethoxyphenol
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sapidus
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lignin-derived
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paper production
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degradation
- 1.11.1.19
- peroxidases
- heme
- lignin
- auricula-judae
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thanatephorus
- cucumeris
-
veratryl
-
auricularia
- irpex
-
ligninolytic
- lacteus
-
lignin-degrading
-
kraft
- adusta
- bjerkandera
- chlorite
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nonphenolic
- 2,6-dimethoxyphenol
- sapidus
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lignin-derived
- paper production
- degradation
Reaction
Synonyms
AnaPX, AncDyPD-b1, DtpA, dye decolorizing peroxidases type B, dye-decolorizing peroxidase, DyP, DyP I, DyP II, DyP-I, DyP-type peroxidase, DyP-V, DyP1, DyP1B, DyP2, DyP3, DyP4, DyPA, EfeB, LiP BA45, LiP-SN, manganese-independent peroxidase I, manganese-independent peroxidase II, MnP BA30, POX, reactiveblue-5: hydrogen-peroxide oxidoreductase, TT1485, tyrA, YcdB, YfeX, YRW2 Mb, YwbN
ECTree
1.11.1.19 dye decolorizing peroxidaseAdvanced search results
results (
results (Temperature Stability
Temperature Stability on EC 1.11.1.19 - dye decolorizing peroxidase
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TEMPERATURE STABILITY 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
15 - 70
after 5 min incubation, respectively, the enzyme shows 100% activity between 15 and 25°C, about 85% activity at 30-35°C, about 75% activity at 40-45°C, about 62% activity at 50°C, and less than 30% activity at 55°C and above
20 - 60
30 - 40
30 - 60
30 - 65
30 - 70
30 - 80
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the enzyme remains stable at temperatures of up to 80°C for 60 min
40
40 - 60
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the half-life times of the enzyme in the absence of 5 mM of Mg2+ are 6, 4, and 2 h at 40, 50, and 60°C, respectively
50
60
60 - 80
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the half-life times of the enzyme in the absence of 5 mM of Mn2+ are 8, 5, and 2 h at 60, 70, and 80°C, respectively. The half-life time of the purified peroxidase at 60, 70, and 80°C increases to 10, 7, and 4 h in the presence 5 mM of MnSO4, respectively
70
20 - 60
the enzyme is relatively stable at temperatures from 20 to 60°C with a residual activity of more than 80%. At higher temperatures, a high loss of activity is observed due to protein denaturation, resulting in residual activities of less than 5%
20 - 60
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the activity of isozyme DyP3 is less than that of isozymes DyP2 and DyP1 at 35°C and 40°C. Isozymes DyP1, DyP2 and DyP3 exhibit approximately 75% activity at 20°C and about 90% activity at 30°C. Isozyme DyP1 shows about 75% activity at 40°C, isozyme DyP2 shows about 85% activity at 40°C, and isozyme DyP3 shows about 50% activity at 40°C. Isozymes DyP1, DyP2 and DyP3 show about 10% activity at 50°C and are completely inactivated at 60°C
30 - 40
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the enzyme is stable at a temperature of 30°C for 1 h, but a sharp decrease is detected after the incubation at 40°C. The enzyme shows almost no activity at 60°C
30 - 40
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the enzyme is stable at a temperature of 30°C for 1 h, but a sharp decrease is detected after the incubation at 40°C. The enzyme shows almost no activity at 60°C
30 - 60
the enzyme is considerably stable at 30°C and 40°C where it retains more than 90% of its activity, while the enzyme loses more than 90% of its activity after incubation at 50°C and 60°C for 3 h
30 - 60
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DyP activity is restored at 30°C after treatment at 40 and 50°C for 11 h, DyP activity is stable after incubation at 50°C for 11 h. When DyP is incubated at 30 and 40°C for 14 days, the inactivation rates are 37 and 59%, respectively. When DyP is heated at 60°C for 3 h, 35% of the initial activity is lost
30 - 60
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the enzyme retains almost of its initial activity against ABTS and DMP after incubation at 30°C and 40°C for 120 min. The peroxidase activity significantly decreases after incubating at 50°C for 60 min, and is almost completely lost following incubation at 60°C for 30 min
30 - 60
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the enzyme retains almost all of its initial activity against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,6-dimethoxyphenol after incubation at 30°C or 40°C for 2 h. The peroxidase activity of the enzyme against ABTS significantly decreases after incubating at 50°C for 1 h and is almost completely lost following incubation at 60°C for 30 min. On the other hand, the activity of the enzyme against 2,6-dimethoxyphenol is maintained at 50°C
30 - 60
after incubation for 2 h at 60°C the enzyme loses 50% of its original activity, while at 30°C and 40°C DyP retains its activity after this period. Upon incubation at 60°C, an almost 2fold increase in activity is observed within 10 min
30 - 65
the wild type enzyme remains stable after 24 h at 30-55°C and shows 50% activity after 24 h at 65.5°C
30 - 65
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the T50 index is adjusted to 48.3°C, after 10 min incubation between 30 and 65°C
30 - 70
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after 2 h heat treatment, the native enzyme shows 97% residual activity at 40°C, 93% residual activity at 50°C, and 79% residual activity at 60°C when measured at 30°C for 1 min. After heating at 30, 40, and 50°C for 120 min, recombinant DyP retains more than 90% of its activity when measured at 30°C for 1 min, but the enzyme gradually becomes inactive after treatment at 60°C (12% residual activity) and is rapidly inactivated at 70°C
30 - 70
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recombinant DyP expressed in Escherichia coli does not lose its activity at 30°C and 40°C. The activity is loweres slightly to 83% of full activity after heating 1 h at 50°C. In contrast, the activity drastically declines at 60°C and 70°C. The residual activity is 4% and 10% of full activity after heating 2 h at 60°C and 15 min at 70°C, respectively. The activity of native DyP after 1 h heating at 60°C is 92% of full activity whereas that of recombinant DyPs expressed in Escherichia coli and Aspergillus oryzae are only 42%, and 44%, respectively
50
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the enzyme shows a residual activity of ca. 80% after incubation at 50°C for 1 h. The enzyme shows almost no activity at 60°C
50
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the enzyme shows a residual activity of ca. 80% after incubation at 50°C for 1 h. The enzyme shows almost no activity at 60°C
60
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the half-life times of the enzyme in the absence of 5 mM of Mg2+ are 6, 4, and 2 h at 40, 50, and 60 °C, respectively. The half-life time of the enzyme at 40, 50, and 60°C increases to 7, 5, and 3 h in the presence 5 mM of Mg2+, respectively
60
the enzyme retains about 70% maximal activity after incubation at 60°C for 1 h
70
the enzyme shows more than 60% activity after incubation at 70°C for 2 h at pH 7.0
