1.11.1.19: dye decolorizing peroxidase

This is an abbreviated version, for detailed information about dye decolorizing peroxidase, go to the full flat file.

Reaction

Reactive Blue 5
+ 2 H2O2 =
Phthalate
+
2,2'-disulfonyl azobenzene
+
3-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino]benzenesulfonate
+ 2 H2O

Synonyms

AnaPX, dye-decolorizing peroxidase, DyP, DyP I, DyP II, DyP-I, DyP-type peroxidase, DyP1, DyP2, DyP3, EfeB, LiP BA45, LiP-SN, manganese-independent peroxidase I, manganese-independent peroxidase II, TT1485, tyrA, YcdB, YfeX, YwbN

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.19 dye decolorizing peroxidase

Temperature Stability

Temperature Stability on EC 1.11.1.19 - dye decolorizing peroxidase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15 - 70
after 5 min incubation, respectively, the enzyme shows 100% activity between 15 and 25°C, about 85% activity at 30-35°C, about 75% activity at 40-45°C, about 62% activity at 50°C, and less than 30% activity at 55°C and above
20 - 60
-
the activity of isozyme DyP3 is less than that of isozymes DyP2 and DyP1 at 35°C and 40°C. Isozymes DyP1, DyP2 and DyP3 exhibit approximately 75% activity at 20°C and about 90% activity at 30°C. Isozyme DyP1 shows about 75% activity at 40°C, isozyme DyP2 shows about 85% activity at 40°C, and isozyme DyP3 shows about 50% activity at 40°C. Isozymes DyP1, DyP2 and DyP3 show about 10% activity at 50°C and are completely inactivated at 60°C
30 - 60
the enzyme is considerably stable at 30°C and 40°C where it retains more than 90% of its activity, while the enzyme loses more than 90% of its activity after incubation at 50°C and 60°C for 3 h
30 - 65
-
the wild type enzyme remains stable after 24 h at 30-55°C and shows 50% activity after 24 h at 65.5°C
30 - 60
-
DyP activity is restored at 30°C after treatment at 40 and 50°C for 11 h, DyP activity is stable after incubation at 50°C for 11 h. When DyP is incubated at 30 and 40°C for 14 days, the inactivation rates are 37 and 59%, respectively. When DyP is heated at 60°C for 3 h, 35% of the initial activity is lost
30 - 70
-
after 2 h heat treatment, the native enzyme shows 97% residual activity at 40°C, 93% residual activity at 50°C, and 79% residual activity at 60°C when measured at 30°C for 1 min. After heating at 30, 40, and 50°C for 120 min, recombinant DyP retains more than 90% of its activity when measured at 30°C for 1 min, but the enzyme gradually becomes inactive after treatment at 60°C (12% residual activity) and is rapidly inactivated at 70°C
30 - 40
30 - 60
-
the enzyme retains almost of its initial activity against ABTS and DMP after incubation at 30°C and 40°C for 120 min. The peroxidase activity significantly decreases after incubating at 50°C for 60 min, and is almost completely lost following incubation at 60°C for 30 min
30 - 70
-
recombinant DyP expressed in Escherichia coli does not lose its activity at 30°C and 40°C. The activity is loweres slightly to 83% of full activity after heating 1 h at 50°C. In contrast, the activity drastically declines at 60°C and 70°C. The residual activity is 4% and 10% of full activity after heating 2 h at 60°C and 15 min at 70°C, respectively. The activity of native DyP after 1 h heating at 60°C is 92% of full activity whereas that of recombinant DyPs expressed in Escherichia coli and Aspergillus oryzae are only 42%, and 44%, respectively
30 - 60
after incubation for 2 h at 60°C the enzyme loses 50% of its original activity, while at 30°C and 40°C DyP retains its activity after this period. Upon incubation at 60°C, an almost 2fold increase in activity is observed within 10 min
40
-
the enzyme activity remains stable for 53 h at 40°C
40 - 60
-
the half-life times of the enzyme in the absence of 5 mM of Mg2+ are 6, 4, and 2 h at 40, 50, and 60°C, respectively
40
-
the enzyme activity remains stable for 53 h at 40°C