1.11.1.19: dye decolorizing peroxidase

This is an abbreviated version!
For detailed information about dye decolorizing peroxidase, go to the full flat file.

Word Map on EC 1.11.1.19

Reaction

Reactive Blue 5
+ 2 H2O2 =
Phthalate
+
2,2'-disulfonyl azobenzene
+
3-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino]benzenesulfonate
+ 2 H2O

Synonyms

AnaPX, dye-decolorizing peroxidase, DyP, DyP I, DyP II, DyP-I, DyP-type peroxidase, DyP1, DyP2, DyP3, EfeB, LiP BA45, LiP-SN, manganese-independent peroxidase I, manganese-independent peroxidase II, TT1485, tyrA, YcdB, YfeX, YwbN

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.19 dye decolorizing peroxidase

Temperature Stability

Temperature Stability on EC 1.11.1.19 - dye decolorizing peroxidase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15 - 70
after 5 min incubation, respectively, the enzyme shows 100% activity between 15 and 25C, about 85% activity at 30-35C, about 75% activity at 40-45C, about 62% activity at 50C, and less than 30% activity at 55C and above
20 - 60
-
the activity of isozyme DyP3 is less than that of isozymes DyP2 and DyP1 at 35C and 40C. Isozymes DyP1, DyP2 and DyP3 exhibit approximately 75% activity at 20C and about 90% activity at 30C. Isozyme DyP1 shows about 75% activity at 40C, isozyme DyP2 shows about 85% activity at 40C, and isozyme DyP3 shows about 50% activity at 40C. Isozymes DyP1, DyP2 and DyP3 show about 10% activity at 50C and are completely inactivated at 60C
30 - 60
the enzyme is considerably stable at 30C and 40C where it retains more than 90% of its activity, while the enzyme loses more than 90% of its activity after incubation at 50C and 60C for 3 h
30 - 65
-
the wild type enzyme remains stable after 24 h at 30-55C and shows 50% activity after 24 h at 65.5C
30 - 60
-
DyP activity is restored at 30C after treatment at 40 and 50C for 11 h, DyP activity is stable after incubation at 50C for 11 h. When DyP is incubated at 30 and 40C for 14 days, the inactivation rates are 37 and 59%, respectively. When DyP is heated at 60C for 3 h, 35% of the initial activity is lost
30 - 70
-
after 2 h heat treatment, the native enzyme shows 97% residual activity at 40C, 93% residual activity at 50C, and 79% residual activity at 60C when measured at 30C for 1 min. After heating at 30, 40, and 50C for 120 min, recombinant DyP retains more than 90% of its activity when measured at 30C for 1 min, but the enzyme gradually becomes inactive after treatment at 60C (12% residual activity) and is rapidly inactivated at 70C
30 - 40
30 - 60
-
the enzyme retains almost of its initial activity against ABTS and DMP after incubation at 30C and 40C for 120 min. The peroxidase activity significantly decreases after incubating at 50C for 60 min, and is almost completely lost following incubation at 60C for 30 min
30 - 70
-
recombinant DyP expressed in Escherichia coli does not lose its activity at 30C and 40C. The activity is loweres slightly to 83% of full activity after heating 1 h at 50C. In contrast, the activity drastically declines at 60C and 70C. The residual activity is 4% and 10% of full activity after heating 2 h at 60C and 15 min at 70C, respectively. The activity of native DyP after 1 h heating at 60C is 92% of full activity whereas that of recombinant DyPs expressed in Escherichia coli and Aspergillus oryzae are only 42%, and 44%, respectively
30 - 60
after incubation for 2 h at 60C the enzyme loses 50% of its original activity, while at 30C and 40C DyP retains its activity after this period. Upon incubation at 60C, an almost 2fold increase in activity is observed within 10 min
40
-
the enzyme activity remains stable for 53 h at 40C
40 - 60
-
the half-life times of the enzyme in the absence of 5 mM of Mg2+ are 6, 4, and 2 h at 40, 50, and 60C, respectively
40
-
the enzyme activity remains stable for 53 h at 40C