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1.11.1.24: thioredoxin-dependent peroxiredoxin

This is an abbreviated version!
For detailed information about thioredoxin-dependent peroxiredoxin, go to the full flat file.

Word Map on EC 1.11.1.24

Reaction

2 R'-SH +

ROOH
=
R'-S-S-R'
 +
H2O
 +
ROH

Synonyms

1-Cys peroxiredoxin, 1-Cys Prx, 1-Cys type Prx, 2-Cys peroxiredoxin, 2-Cys peroxiredoxin 4, 2-Cys Prx, 2-Cys type Prx, 25 kDa thiol-specific oxidant, 2Cys-peroxiredoxin, AbTPx1, AbTPx2, Ac-1-Cys Prx, Ahp, Ahp1, AhpC, AhpC-like peroxiredoxin, AhpC-like Prx, AhpC2, AhpE, alkyl hydroperoxide reductase, alkyl hydroperoxide reductase C component, alkyl hydroperoxide reductase subunit C, alkylhydroperoxide reductase subunit C, APE2278, ApTPx, AsPrx, atypical two-cysteine peroxidase, bacterioferritin comigratory protein, BCP, Bcp1, Bcp3, Bcp4, BiPrx1, BiTPx1, BmTPx-Q, C-PrxII, C2C-Prx, calpromotin, CIC-Prx, cPrx I, cPrx II, CPX, DTT-dependent peroxidase, EC 1.11.1.15, EcTpx, EgTPx, FhePrx, GPX, HBP23/Prx I, heme-binding protein 23/peroxiredoxin, LimTXNPx, More, MPX, MtTPx, natural killer enhancing factor-B, ncgl2403, NES-Prx1, NLS-Prx1, nuclear export signal-Prx1, nuclear localization signal-Prx1, peroxiredoxin, peroxiredoxin 1, peroxiredoxin 2, peroxiredoxin 3, peroxiredoxin 4, peroxiredoxin 5, peroxiredoxin 6, peroxiredoxin I, peroxiredoxin II, peroxiredoxin III, peroxiredoxin IV, peroxiredoxin Q, peroxiredoxin V, peroxiredoxin-1, peroxiredoxin-3, peroxiredoxin-4, PfTrx-Px1, PfTrx-Px2, PH1217, PH1217 protein, PRDX I, PRDX II, PRDX III, PRDX-2, PRDX-3, PRDX2, PRDX5, Prdx6, Prx, Prx 2, Prx 3, Prx 4, Prx I, Prx II, Prx III, PRx IV, Prx Q, Prx Q1, Prx Q2, Prx V, Prx VI, Prx-4, Prx1, Prx2, Prx3, Prx5, PrxII, PrxII F, PrxQ, PrxT, PrxV, PrxVI, Rv2238c, SAOUHSC_01822, SF2523, sll0221, sll0755, sll1621, slr0242, slr1198, SSO2613, tgTPx1/2, TgTrx-Px1, TgTrx-Px2, thiol-specific antioxidant/protector protein, thioredoxin peroxidase, thioredoxin peroxidase 1, thioredoxin peroxidase 1/2, thioredoxin peroxidase 2, thioredoxin peroxidase B, thioredoxin peroxidase II, thioredoxin peroxidase TSA2, thioredoxin-dependent alkyl hydroperoxide reductase, thioredoxin-dependent peroxiredoxin Q, thioredoxin-dependent thiol peroxidase, TM0807, torin, TP0509, Tpx, TPx I, TPx II, TPx-1, TPx-B, TPx1, TPx1/2, TPX2, Tpx3, TRIREDRAFT_47136, tryparedoxin peroxidase, tryparedoxin/peroxynitrite oxidoreductase, Ts2-CysPrx, TSA, TSA thioredoxin peroxidase Tpx, Tsa1, TsaA, two-cysteine peroxiredoxin, TXNPx, type II peroxiredoxin, typical 2-Cys peroxiredoxin, typical 2-Cys Prx

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.24 thioredoxin-dependent peroxiredoxin

Purification

Purification on EC 1.11.1.24 - thioredoxin-dependent peroxiredoxin

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, ACA44 gel filtration, and DEAE Sephacel column chromatography
-
amylose resin column chromatography
DE-52 column chromatography and 2',5'-ADP-Sepharose affinity column chromatography
DEAE-Sepharose column chromatography, SP-Sepharose column chromatography, and Superdex 200 gel filtration
DEAE-Sepharose column chromatography, Superdex 75 gel filtration, and Superdex 200 gel filtration
-
glutathione-Sepharose column chromatography, gel filtration
-
GSH-Sepharose 4B bead chromatography
HiPrep 16/10 DEAE column chromatography
HisTrap HP column chromatography, Resource S column chromatography, and Resource Q column chromatography
Ni-NTA column chromatography and Superdex 75 gel filtration
Ni-NTA resin column chromatography and gel filtration
Ni-NTA resin column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration
-
Ni-NTA Sepharose column chromatography
Ni-Sepharose column chromatography
Ni2+ column chromatography
Ni2+-iminodiacetate-Sepharose column chromatography
nickel-chelate affinity column chromatography
phenyl-Sepharose column chromatography, S-Sepharose column chromatography, hydroxyapatite column chromatography, and G25 gel filtration
recombinant enzyme
recombinant enzyme versions PRX264, Prx231, and Prx197 from Escherichia coli strain BL21(DE3)
recombinant His-tagged enzyme from Escherichia coli by nickle affinity chromatography and gel filtration
recombinant Prx 2 from Escherichia coli by nickel affinity chromatography and gel filtration
Sepharose column chromatography, and Superdex 200 gel filtration
TALON metal affinity resin column chromatography
wild-type and mutant enzymes C44S and C49S
with chitin beads
-