Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.11.1.6: catalase

This is an abbreviated version!
For detailed information about catalase, go to the full flat file.

Word Map on EC 1.11.1.6

Reaction

2 H2O2 =

O2
+ 2 H2O

Synonyms

Ab-catalase, BNC, caperase, CAT, CAT-1, CAT-A, CAT-P, Cat1.4, CatA, catalase, catalase A, catalase C, catalase form III, catalase P, catalase-1, catalase-A, catalase-peroxidase, catalase-phenol oxidase, CatB, CATC, CatF, CatG, CatP, CATPO, CcmC, CP, equilase, H2O2:H2O2 oxidoreductase, haem catalase, HPI-A, HPI-B, HPII, HTHP, hydrogen peroxide oxidoreductase, KAT, Kat E catalase, KatA, KatB, KatC, KatP, KpA, manganese catalase, More, optidase, PktA, polyethylene glycol-catalase, tyrosine-coordinated heme protein, VktA

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.6 catalase

Temperature Stability

Temperature Stability on EC 1.11.1.6 - catalase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 70
-
when the extract is incubated at specified temperature (25-70°C) for various length of time (5-30 min) then tested at room temperature a decrease in catalase activity is found at temperature above 50, 60, and 70°C for pHs of 6.5, 10.0, and 4.5, respectively. After heating at 70°C for 30 min, catalase activity loses about 40, 60, and 75% of the original activity at pH 4.5, 10.0, and 6.5, respectively.
30 - 60
-
thermoinactivation kinetics in the presence or absence of substrate H2O2, overview
35 - 40
-
slightly suppressed activity
35 - 50
-
the half-lives of free catalase at 35 and 50°C were 9.0 and 6.7 h, respectively and these correspondingly are 70.0 and 9.7 h for immobilized catalase onto controlled pore glass
40 - 50
-
the half lives of free catalase at 40 and 50°C are 9.0 and 6.7 h, respectively, and correspondingly 29.1 and 4.8 h for Eupergit C-immobilized catalase
40 - 60
-
the enzyme is stable for 24 h at 40°C with a half-life of 4 h 60°C, at 50°C the half life is about 18 h
50 - 60
-
65% residual activity after 15 min at 50°C, 54% residual activity after 30 min at 50°C, 43% residual activity after 40 min at 50°C, 12% residual activity after 15 min of preincubation at 60°C, preincubation at 65°C for 10 min results in complete loss of activity
58
-
1 h, 83% reduction in activity
60 - 70
at 60°C, the enzyme is stable for 240 min, at 65°C the enzyme retains more than 55% of its activity by incubating the enzyme at pH 7.0 after 225 min, at 70°C, the enzyme can retain 57% of its initial activity after incubating at pH 7.0 for 90 min
68
5 min, no loss of activity
95
half-life: 114 min
additional information
high thermal stability. HTHP is stable under a wide range of temperatures. Thermal unfolding is measured up to 110°C and in the presence of high concentrations of guanidinium hydrochloride. The melting point of HTHP is estimated to be around 130°C. Catalatic activity is tested after incubation for 10 min at 85°C and 90°C and is found to be reduced by less than 5% under both conditions