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1.11.1.7: peroxidase

This is an abbreviated version!
For detailed information about peroxidase, go to the full flat file.

Word Map on EC 1.11.1.7

Reaction

2 phenolic donor +

H2O2
= 2 phenoxyl radical of the donor + 2 H2O

Synonyms

acidic peroxidase, acidic POD, ALSP, AnaPX, anionic isoperoxidase, anionic peroxidase A1, AOPTP, basic peroxidase, basic POD, BCcP, black radish peroxidase A, black radish peroxidase B, BRP-A, BRP-B, cationic peroxidase, cationic peroxidase Cs, class III plant peroxidase, CmMnP, constitutive peroxidase, CP, cPOD-I, CYP119, dehaloperoxidase A, dehaloperoxidase B, DHP A, donor: H2O2 oxidoreductase, donor: hydrogen peroxide oxidoreductase, donor:hydrogen peroxide oxidoreductase, DtpA, ECPOX, ECPOX 1, ECPOX 2, ECPOX 3, ELP, eosinophil peroxidase, EPO, extensin peroxidase, extracellular peroxidase, FP1, Fp2, FP3, GCP1, GCP2, guaiacol peroxidase, H2O2 oxidoreductase, Hb peroxidase, Hb1, heme peroxidase, hemoglobin 1, hemoglobin peroxidase, hexacoordinate (class 1) non-symbiotic hemoglobin, horseradisch peroxidase, horseradish peroxidase, horseradish peroxidase (HRP), horseradish peroxidase C, HP, HRP, HRP A2, HRP C1A, HRP-C, HRPC, HRPO, HRP_A2A, HRP_C1A, HRP_E5, HTHP, hydrogen donor oxidoreductase, Japanese radish peroxidase, lactoperoxidase, LPO, LPRX, LPS, MAP-2744c, MGP, MnP124076, MnP13, More, MPO, myeloperoxidase, neutral peroxidase, neutral POD, NGO_0994, nsHb-1, oxyperoxidase, p20, PA1, PerII, peroxidase, peroxidase isoenzyme E5, POC1, POD, POD1, POII, POX, POX I, POX II, POX2, protoheme peroxidase, Prx02, Prx03, Prx06, Prx07, Prx09, Prx1, Prx11, Prx114, Prx12, Prx13, Prx15, Prx17, Prx21, Prx22, Prx27, Prx28, Prx30, Prx31, Prx32, Prx33, Prx34, Prx36, Prx37, Prx39, Prx42, Prx43, Prx45, Prx49, Prx50, Prx51, Prx52, Prx53, Prx55, Prx56, Prx57, Prx58, Prx59, Prx60, Prx62, Prx64, Prx66, Prx67, Prx68, Prx69, Prx70, Prx71, Prx72, Prx73, pyrocatechol peroxidase, QPO, quinol peroxidase, rhEPO, rHRP1, rHRP2, royal palm tree peroxidase, rPOD-II, RPTP, rubrerythrin, SacD, Saci_2081, SBP, scavengase, scopoletin peroxidase, SfmD, short PMnP, SPC4, Ssp, stigma-specific peroxidase, thiocyanate peroxidase, thiol peroxidase, TOP, TP I, Tpx, tyrosine-coordinated heme protein, vacuolar class III peroxidase, vascular peroxidase 1, verdoperoxidase, versatile peroxidase, versatile peroxidase VPL2, VP, VPO1, VPO2, WPTP

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.7 peroxidase

Purification

Purification on EC 1.11.1.7 - peroxidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Amberlite CG-50 resin column chromatography, CM-Sephadex C-50 column chromatography, and Sephadex G-100 gel filtration
-
Amberlite CG50, CM Sephadex, Sephadex G-100
-
ammonium sulfate fractionation and DEAE cellulose column chromatography
-
ammonium sulfate precipitation and Con A Sepharose column chromatography
-
ammonium sulfate precipitation and four chromatographic steps
ammonium sulfate precipitation and Sephadex G-200 gel filtration
ammonium sulfate precipitation, affinity chromatography, and gel filtration
-
ammonium sulfate precipitation, Con A-Sepharose column chromatography, and DEAE-cellulose column chromatography
-
ammonium sulfate precipitation, DEAE-cellulose chromatography, carboxymethyl-cellulose column chromatography, and Sephacryl S-200 gel filtration
-
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, ether-Toyopearl column chromatography, and Sephacryl S-200 gel filtration
-
ammonium sulfate precipitation, dialysis, and CM-Sephadex A-50 gel filtration
-
ammonium sulfate precipitation, hydrophobic chromatography Octyl-Sepharose 4 Fast Flow column, and molecular exclusion chromatography Sephacryl S-200 HR column
-
ammonium sulfate precipitation, ion exchange, and gel filtration chromatography, 79fold purification
-
ammonium sulfate precipitation, ion-exchange chromatography and gel filtration
-
ammonium sulfate precipitation, phenyl-Sepharose column chromatography, DEAE-Toyopearl column chromatography, and Superdex 200 gel filtration
-
ammonium sulfate precipitation, Sephadex G-25 gel filtration, Red Sepharose CL-6B affinity chromatography, HyperDQ high-performance liquid chromatography, and isoelectrofocusing
ammonium sulfate, DEAE-Sephacel, Sephadex G-100
-
ammonium sulfate, DEAE-Sephacel, Sephadex G-100, Phenyl-Sepharose
-
ammonium sulfate, DEAE-Sephacel, SP-Sepharose, Sephadex G-100, Phenyl-Sepharose
-
ammonium sulfate, gel filtration, anion exchange
Elais guineensis
-
ammonium sulfate, ion exchange chromatography (DEAE-Sepharose) and gel filtration (Sephcryl S-200)
-
ammonium sulfate, Phenyl-Sepharodse, Q-Sepharose
both soluble and membrane-bound isoform
-
cation exchange, hydrophobic interaction chromatography
-
CM-Sephadex C-50 gel filtration and Sephadex G-100 gel filtration
-
CM-Sephadex gel filtration and Sephadex G-100 gel filtration
-
DEAE Sepharose column chromatography
DEAE-Toyopearl column chromatography, ammonium sulfate precipitation, butyl-Toyopearl column chromatography, and hydroxylapatite column chromatography
-
from fruit
-
from root
-
G25 Sephadex gel filtration, Q-Sepharose column chromatography
-
hydrophobic charge induction chromatography with 4-mercapto-ethyl-pyridine and Superdex 75 gel filtration
-
intracellular enzyme 476fold to homogeneity from phosphate-starved cell culture by PEG 8000 treatment, adsorption chromatography, gel filtration, and concanavalin A affinity chromatography
-
ion exchange chromatography, gel filtration
-
isoenzyme POX I
-
isoenzyme POX II
-
isoenzyme POX2
-
m-aminophenylboronic acid agarose column chromatography
-
MonoQ column chromatography, DEAE-FF Sepharose column chromatography, and concanavalin A column chromatography
native and recombinant enzyme
-
Ni-NTA affinity column chromatography
-
Ni-NTA column chromatography and MonoQ column chromatography
Ni-NTA column chromatography, TALON Co2+ affinity column chromatography, and Superdex 200 gel filtration
-
Ni2+ HisTrap column chromatography
partial purification, using a combination of phase partitioning with Triton X-114 and ammonium sulfate fractionation between 30 and 80%
-
partially purified, ammonium sulfate, acetone
-
PHEMABA cryogel membranes are promising materials in large-scale industrial applications since they are easy to prepare and reusable materials and have high affinity to peroxidase reaching up to high binding capacities with high purification
-
Phenyl-sepharose, Sephacryl S200, DEAE-cellulose
-
protein extraction by a combination of freeze-thawing and grinding, ultrafiltration and diafiltration, ammonium sulfate precipitation, DEAE anion-exchange chromatography, and concanavalin A affinity chromatography
-
purified to homogeneity as holoprotein by affinity chromatography
Q Sepharose column chromatography and Mono Q column chromatography
-
Q-Sepharose, metal chelate, superose-12
-
recombinant enzyme
-
recombinant enzyme, 6 M urea, reactivation, ultrafiltration, gel filtration
-
recombinant soluble His-tagged enzyme from Escherichia coli strain BL21(DE3) in presence of 1 mM CaCl2 by nickel affinity chromatography, dialysis, followed by anion exchange chromatography, ultrafiltration, and gel filtration. Heme incorporation into MnP after purification and removal of excess heme by high speed centrifugation at 50000 x g
reverse micellar extraction with sodium di(2-ethylhexyl)sulfosuccinate 0.1-0.3 M dissolved in iso-octane
-
salt precipitation, dialysis, gel filtrations and ion exchange chromatographic techniques
-
Sepharose 4B-L-tyrosine affinity column chromatography
-
single step purification using 4-amino benzohydrazide affinity chromatography
Brassica oleracea var. capitata f. rubra
-
Toyopearl-SP column chromatography
-
ultrasonication, ammonium sulfate salt precipitation, and phenyl Sepharose column chromatography
-
wild-type and mutant enzymes