1.11.1.9: glutathione peroxidase

This is an abbreviated version!
For detailed information about glutathione peroxidase, go to the full flat file.

Reaction

2 glutathione +

Synonyms

2-SeCD, 3,3'-telluro-bis(proapne-3,1-diyl)adamantane carboxylate, 6P229, ADA-Te-OH, ARMEP24, AtGPX1, ATGPX3, c-GPx4, Cellular glutathione peroxidase, cgd3_460, cGPx, Cuticular glycoprotein GP29, DI29, ebselen, EGLP, Epididymis-specific glutathione peroxidase-like protein, Extracellular glutathione peroxidase, G-6137, Gastrointestinal glutathione peroxidase, glutathione peroxidase 1, glutathione peroxidase 2, glutathione peroxidase 3, glutathione peroxidase 4, glutathione peroxidase Gpx2, glutathione peroxidase Gpx3, glutathione peroxidase-1, glutathione peroxidase-2, glutathione peroxidase-3, glutathione peroxidase-4, glutathione-dependent peroxidase I, glutathione:hydrogen-peroxide oxidoreductase, GP30, GPRP, GPX, Gpx-1, GPx-2, GPx-3, GPx-4, GPx-5, GPx-6, GPx-7, GPx-8, GPX1, Gpx2, GPX3, GPx4, GPX5, GSH peroxidase, GSH-Px, GSHPx, GSHPx-GI, intracellular GpX, m-GPx4, Major androgen-regulated protein, Major surface antigen GP29, n-GPx4, Nt-SubC08, Odorant-metabolizing protein RY2D1, Os02g0664000, OsGPX5, peroxidase, glutathione, PGdx, pGPx, PHGPX, phospholipid glutathione peroxidase, phospholipid hydroperoxide glutathione peroxidase, phospholipid hydroperoxide glutathione peroxidase, nuclear form, phospholipid hydroperoxide Gpx, plasma glutathione peroxidase, prion protein Ure2, reduced glutathione peroxidase, Salt-associated protein, Se-GPx, Se-hGSTZ1-1, Se-scFv-B3, secreted GpX, SeGPx, selenium containing glutathione transferase zeta1-1, selenium-dependent glutathione peroxidase, selenium-glutathione peroxidase, seleno-dependent glutathione peroxidase 1, selenosubtilisin, snGPx, TcGPXI, TTHERM_00046090, TTHERM_00046110, TTHERM_01099010, Ure2

ECTree

     1 Oxidoreductases

         1.11 Acting on a peroxide as acceptor

             1.11.1 Peroxidases

                1.11.1.9 glutathione peroxidase

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Results	in table
18	Activating Compound
1510	AA Sequence
12	Application
1	CAS Registry Number
29	Cloned(Commentary)
9	Crystallization (Commentary)
13419	Disease/ Diagnostics
27	Expression
20	General Information
7	General Stability
77	Inhibitors
4	kcat/KM [mM/s]
4	Ki Value [mM]
63	KM Value [mM]
90	Localization
62	Metals/Ions
52	Molecular Weight [Da]
122	Natural Substrates/ Products (Substrates)
2	Organic Solvent Stability
228	Organism
2	Oxidation Stability
7	Pathway
25	PDB ID
20	pH Optimum
5	pH Range
7	pH Stability
4	pI Value
2	Posttranslational Modification
15	Protein Variants
39	Purification (Commentary)
3	Reaction
3	Reaction Type
199	Reference
269	Source Tissue
45	Specific Activity [micromol/min/mg]
8	Storage Stability
253	Substrates and Products (Substrate)
62	Subunits
216	Synonyms
1	Systematic Name
11	Temperature Optimum [°C]
5	Temperature Range [°C]
4	Temperature Stability [°C]
23	Turnover Number [1/s]

Engineering

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Engineering on EC 1.11.1.9 - glutathione peroxidase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

C74A

Drosophila melanogaster

Q9VZQ8

mutation results in a decline of specific activity

684497

N136A

Drosophila melanogaster

Q9VZQ8

mutation results in a dramatic decline of specific activity

684497

N136D

Drosophila melanogaster

Q9VZQ8

mutation results in not detectable specific activity

684497

N136H

Drosophila melanogaster

Q9VZQ8

mutation results in a dramatic decline of specific activity

684497

N40A

Drosophila melanogaster

Q9VZQ8

mutation results in a decline of specific activity

684497

C2S/C78S/C115S/C156S/C202S

Homo sapiens

P07203

about 10fold increase in activity compared to wild-type. Mutant displays a typical ping-pong mechanism

742715

S14A

Homo sapiens

-

mutant shows reduced selenium content and strongly reduced glutathione peroxidase activity

687123

S15A

Homo sapiens

-

mutant shows reduced selenium content and reduced glutathione peroxidase activity

687123

U43C

Schistosoma mansoni

Q00277

inactive

701138

U43S

Schistosoma mansoni

Q00277

inactive

701138

additional information

7 entries

additional information

Homo sapiens

-

full-length (1-201) and C-terminal deletion (1-100) versions of human GPX1 (Sec47Cys), full-length FLAG-GPX1 is modified with O-GlcNAc, while the truncated FLAG-GPX1 is not, despite the fact that truncated GPX1 is still able to bind OGT. O-GlcNAcylation occurs on the C-terminal half of GPX1

702092

additional information

Homo sapiens

P18283

conversion of the codons for four cysteine residues to the codons for serine residues and change of the codon for Sec40 to the codon for Cys. The recombinant seleno-hGPx2 mutant is obtained using a single protein production system in a cysteine auxotrophic strain, Sec is introduced into the protein via tRNACys misleading. The activity of this mutant is in the same order of magnitude as that of isoform hGPx4, but about one order of magnitude lower than that of hGPx1 and hGPx3. The mutant follows a typical ping-pong mechanism similar to native GPx

742568

additional information

Homo sapiens

-

conversion of the codons for four cysteine residues to the codons for serine residues and change of the codon for Sec40 to the codon for Cys. The recombinant seleno-hGPx2 mutant is obtained using a single protein production system in a cysteine auxotrophic strain, Sec is introduced into the protein via tRNACys misleading. The activity of this mutant is in the same order of magnitude as that of isoform hGPx4, but about one order of magnitude lower than that of hGPx1 and hGPx3. The mutant follows a typical ping-pong mechanism similar to native GPx

742568

additional information

Homo sapiens

P22352

expression of a recombinant human Gpx3 mutant with all Cys residues changed to Ser from a Cys auxotrophic strain of E. coli. Although lacking post-translational modification, the mutant still retains the ability to reduce H2O2 and 1-palmitoyl-2-(13-hydroperoxy-cis-9, trans-11-octadecadienoyl)-L-3-phosphatidylcholine. Contrary to wild-type, the mutant exists as a monomer in solution

743791

additional information

Homo sapiens

-

expression of a recombinant human Gpx3 mutant with all Cys residues changed to Ser from a Cys auxotrophic strain of E. coli. Although lacking post-translational modification, the mutant still retains the ability to reduce H2O2 and 1-palmitoyl-2-(13-hydroperoxy-cis-9, trans-11-octadecadienoyl)-L-3-phosphatidylcholine. Contrary to wild-type, the mutant exists as a monomer in solution

743791

additional information

Rattus norvegicus

-

the enzyme has potential therapeutic value as an antioxidant, but its pharmacological development is limited because the enzyme uses a selenocysteine as its catalytic group and it is difficult to generate selenium-containing proteins with traditional recombinant DNA technology. Rat theta-class glutathione transferase T2-2 (rGST T2-2) presents an ideal scaffold for the design of a novel glutathione peroxidase catalyst because it binds GSH and contains a serine close to substrate binding site, which can be chemically modified to bind selenium. The modified Se-rGST T2-2 efficiently catalyzes the reduction of hydrogen peroxide, and the glutathione peroxidase activity surpasses the activities of some natural glutathione peroxidases

658429

additional information

Rattus norvegicus

-

GPX1 modified with O-GlcNAc on its C-terminus, cell lysate of VSMCs treated with the O-GlcNAcase inhibitor PUGNAc

702092