1.12.1.2: hydrogen dehydrogenase

This is an abbreviated version!
For detailed information about hydrogen dehydrogenase, go to the full flat file.

Reaction

Synonyms

bidirectional hydrogenase, bidirectional Ni-Fe hydrogenase, bidirectional NiFe-hydrogenase, bidirectional [NiFe] hydrogenase, dehydrogenase, hydrogen, FeHyd, H2:NAD+ oxidoreductase, H2ase, Hox, Hox hydrogenase, Hox type hydrogenase, HoxE, HoxEFUYH, HoxF, HoxFUYHI2, HoxH, HoxS, HoxU, HoxY, hydrogen-evolving enzyme, hydrogenase, Hyn, iron-hydrogenase, NAD+-reducing hydrogenase, NAD+-reducing NiFe hydrogenase, NAD+-reducing [NiFe]-hydrogenase, NAD-dependent hydrogenase, NAD-reducing hydrogenase, NADH-dependent Fe-only hydrogenase, NiFe hydrogenase, NiFe-type reversible hydrogenase, SH, soluble hydrogenase, soluble [NiFe]-hydrogenase, [FeFe] hydrogenase, [FeFe]-hydrogenase, [NiFe] H2ase, [NiFe] hydrogenase, [NiFe]-hydrogenase

ECTree

     1 Oxidoreductases

         1.12 Acting on hydrogen as donor

             1.12.1 With NAD⁺ or NADP⁺ as acceptor

                1.12.1.2 hydrogen dehydrogenase

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Results	in table
23	Activating Compound
1071	AA Sequence
5	Application
2	CAS Registry Number
8	Cloned(Commentary)
63	Cofactor
1	Crystallization (Commentary)
1	General Information
6	General Stability
30	Inhibitors
2	Ki Value [mM]
38	KM Value [mM]
35	Localization
65	Metals/Ions
25	Molecular Weight [Da]
33	Natural Substrates/ Products (Substrates)
647	Organism
11	Oxidation Stability
4	Pathway
8	PDB ID
22	pH Optimum
3	pH Range
5	pH Stability
1	Posttranslational Modification
40	Protein Variants
18	Purification (Commentary)
6	Reaction
3	Reaction Type
116	Reference
12	Source Tissue
61	Specific Activity [micromol/min/mg]
5	Storage Stability
261	Substrates and Products (Substrate)
31	Subunits
104	Synonyms
1	Systematic Name
14	Temperature Optimum [°C]
4	Temperature Stability [°C]
21	Turnover Number [1/s]

Oxidation Stability

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Oxidation Stability on EC 1.12.1.2 - hydrogen dehydrogenase

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OXIDATION STABILITY

ORGANISM

UNIPROT

LITERATURE

air the presence of NADH leads to rapid destruction of the hexameric enzyme

Cupriavidus necator

-

674924

enzyme is oxygen-labile

Synechocystis sp.

Q9Z354

654821

highly insensitive to O2

Cupriavidus necator

-

673177

insensitive to O2

Cupriavidus necator

-

656298

insensitivity towards oxygen, irreversible enzyme inhibition by oxygen occurs if the CN- bound to Ni2+ is irreversibly removed or if the enzyme is reduced by NADH

Cupriavidus necator

-

656298

oxidation with O2 and ferricyanide in absence of reducing components stabilizes, simultaneous presence of H2, NADH and O2 causes irreversible inactivation, half-lives in H2/O2 mixtures: 5% O2, 60 min, 20% O2, 30 min, 50% O2, 12 min

Cupriavidus necator

-

439708

oxidized hydrogenase purified under aerobic conditions is highly stable but not reactive, reductive activation of the enzyme by H2 in the presence of catalytic amounts of NADH or by reducing agents destabilizes

Cupriavidus necator

-

439699

rapid and irreversible inactivation under aerobic conditiones in the presence of reductants e.g. 1-10 mM NADH

Cupriavidus necator

-

439704

redox-dependent inactivation and activation, proposed inactivation mechanism

Cupriavidus necator

-

439704, 439705

the hexameric SH preparation is both active and stable in the presence of NADPH and air

Cupriavidus necator

-

674924