1.13.11.24: quercetin 2,3-dioxygenase

This is an abbreviated version!
For detailed information about quercetin 2,3-dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.24

Reaction

quercetin
+
O2
=
2-(3,4-dihydroxybenzoyloxy)-4,6-dihydroxybenzoate
+
CO
+
H+

Synonyms

2,3-QD, 2,3QD, 2,4-QD, Co-QDO, Co-QueD, Cu2+-containing 2,4-QD, cupin domain-containing protein, Fe-QDO, Fe-QueD, flavonol 2,4-dioxygenase, flavonol 2,4-oxygenase, manganese quercetin 2,3-dioxygenase, manganese quercetin dioxygenase, Mn-QDO, Mn-QueD, Ni-QueD, nickel quercetinase, pirin, QDO, QdoI, QueD, quercetin 2,4-dioxygenase, quercetin dioxygenase, quercetinase, type III extradiol dioxygenase, VdQase, YxaG

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.24 quercetin 2,3-dioxygenase

Cloned

Cloned on EC 1.13.11.24 - quercetin 2,3-dioxygenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Aspergillus awamori
-
expressed in Escherichia coli BL21(DE3)
-
expressed in Escherichia coli BL21(DE3) cells
-
expression in Escherichia coli
-
expression in Escherichia coli BL21(DE3) (pLysS, pET23a-queD) as a His6-tagged protein
-
expression of Fe-QDO in Escherichia coli
-
gene queD, recombinant expression of C-terminally Strep-tagged wild-type and mutant enzymes in Escherichia coli in CoCl2- or MnCl2-supplemented medium
gene VDAG_02532, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis
overexpression in Escherichia coli or in in Streptomyces lividans TK23