1.13.11.24: quercetin 2,3-dioxygenase

This is an abbreviated version!
For detailed information about quercetin 2,3-dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.24

Reaction

quercetin
+
O2
=
2-(3,4-dihydroxybenzoyloxy)-4,6-dihydroxybenzoate
+
CO
+
H+

Synonyms

2,3-QD, 2,3QD, 2,4-QD, Co-QDO, Co-QueD, Cu2+-containing 2,4-QD, cupin domain-containing protein, Fe-QDO, Fe-QueD, flavonol 2,4-dioxygenase, flavonol 2,4-oxygenase, manganese quercetin 2,3-dioxygenase, manganese quercetin dioxygenase, Mn-QDO, Mn-QueD, Ni-QueD, nickel quercetinase, pirin, QDO, QdoI, QueD, quercetin 2,4-dioxygenase, quercetin dioxygenase, quercetinase, type III extradiol dioxygenase, VdQase, YxaG

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.24 quercetin 2,3-dioxygenase

Specific Activity

Specific Activity on EC 1.13.11.24 - quercetin 2,3-dioxygenase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0012
-
native enzyme, using quercetin as substrate, at 25°C, pH 7.5
0.0276
-
recombinant enzyme, using fisetin as substrate, at 25°C, pH 7.5
1.5
-
25°C, pH 7.5
1.87
-
recombinant enzyme, using tamarixetin as substrate, at 25°C, pH 7.5
137
purified recombinant Ni2+-supplemented enzyme, pH 8.0, 30°C
144
-
Ni-QueD
18
purified recombinant Mn2+-supplemented enzyme, pH 8.0, 30°C
2.27
-
recombinant enzyme, using quercetin as substrate, at 25°C, pH 7.5
2.49
-
recombinant enzyme, using galangin as substrate, at 25°C, pH 7.5
28
-
Co-QueD
30
purified recombinant Co2+-supplemented enzyme, pH 8.0, 30°C
4
-
Fe-QueD
8
purified recombinant Fe2+-supplemented enzyme, pH 8.0, 30°C
additional information
-
high specific QueD activity is found in crude cell extracts when the growth medium is supplemented with NiCl2 or CoCl2, but not when Mn2+, Fe2+, Cu2+, or Zn2+ is added, the activity of the purified QueD proteins depends strongly on the nature of the metal ion cofactor