sequence of the cloned 4-hydroxyphenylpyruvate dioxygenase coincides with published 4-hydroxyphenylpyruvate dioxygenase-sequences from Arabidopsis thaliana to a nearly perfect identity match except for minor differences in the C-terminal parts of the cDNA.
The alpha-tocophenol content is increased in leaves of the transgenic Nicotiana tabacum (tabacco) plants, whereas the transgenic tobacco seeds contain a significantly increased gamma-tocochromanol level. Overexpression of the barley 4-hydroxyphenylpyruvate dioxygenase gene in plastids of tobacco does not prove to be advantageous in comparison to transgenic tobacco plants expressing high 4-hydroxyphenylpyruvate dioxygenase level in the cytoplasma.
4-hydroxyphenylpyruvate dioxygenase of Pseudomonas fluroscens is cloned into Nicotiana tabacum (cv. PBD6) and into Glycine max (cv. Jack) and tobacco plastid transformants acquire a strong herbicide tolerance, performing better than nuclear transformants.