1.13.11.27: 4-hydroxyphenylpyruvate dioxygenase

This is an abbreviated version!
For detailed information about 4-hydroxyphenylpyruvate dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.27

Reaction

4-hydroxyphenylpyruvate
+
O2
=
homogentisate
+
CO2

Synonyms

4-HPPD, 4-hydroxyphenylpyruvare dioxygenase, 4-hydroxyphenylpyruvate dioxygenase, 4-hydroxyphenylpyruvic acid dioxygenase, 4HPPD, At-HPPD, AtHPPD, AvHPPD-03, EC 1.14.2.2, EC 1.99.1.14, formerly, F Alloantigen, F protein, F-antigen homolog, HPD, hpdA, HPPD, HPPDase, Legiolysin, MsHPPD, oxygenase, 4-hydroxyphenylpyruvate di-, p-hydroxyphenyl pyruvate dioxygenase, p-hydroxyphenylpyruvate dioxygenase, p-hydroxyphenylpyruvate hydroxylase, p-hydroxyphenylpyruvate oxidase, p-hydroxyphenylpyruvic acid hydroxylase, p-hydroxyphenylpyruvic hydroxylase, p-hydroxyphenylpyruvic oxidase, PTO1369, Pt_Hpd, T-cell reactive protein, TF-AG, YS103B

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.27 4-hydroxyphenylpyruvate dioxygenase

Storage Stability

Storage Stability on EC 1.13.11.27 - 4-hydroxyphenylpyruvate dioxygenase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, concentrated enzyme solution, 1.0 mg/ml, stable for 6 months or more
-
-20C, concentrated solution, 1 mg/ml, pH 6.0, stable for 6 months or more
-
-20C, on long-term storage the enzyme forms polymers, reversal by thiols
-
-60C, 0.2 M NaCl solution buffered with 10 mM potassium phosphate, pH 6.7, stable for several months
-
-60C, pH 7.5, 3 months, 20% loss of activity when enzyme concentration is 0.5 g/L in Tris/HCl buffer, no loss of activity after 4 years when enzyme concentration is 5 g/l
-
-60C, stable for several months
4C, 10 mM sodium acetate buffer, pH 6.0, little loss of activity after 2 months
-
4C, dilute solutions, 0.1 mg/ml, 100 mM sodium acetate buffer, PH 6.0, stable for up to 2 months
-
storage at room temperature results in residual enzyme activities of ca. 60-80% after 16 h or 40-50% after 30 h.